A sensitive and label-free electrochemical impedance biosensor for protein detection based on terminal protection of small molecule-linked DNA

Wang, Haibo; Zhang, Hongding; Xu, Shuping; Gan, Tian; Huang, Ke‐Jing; Liu, Yanming

doi:10.1016/j.snb.2014.01.002

Cited by 41 publications

(22 citation statements)

References 30 publications

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“…The paper-based discs are disposable while the PCB substrate can be reused. In comparison to other rapid, sensitive and portable label-free biosensors for protein quantification that have recently been reported in the literature [16][17][18][19][20][21][22][23][24][25][26][27][28], the presented method has improved cost-effectiveness, simplification and ease of use. Thus, although an increased upper limit of detection for our presented methodology is suspected, further research should be carried out to determine whether it may be applicable for the measurement of protein biomarkers of a broadened range of concentrations.…”

Section: Analysis and Comparison With Elisa And Recently Developed Mementioning

confidence: 98%

Carbon Nanotube-Based Electrochemical Biosensor for Label-Free Protein Detection

et al. 2019

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There is a growing need for biosensors that are capable of efficiently and rapidly quantifying protein biomarkers, both in the biological research and clinical setting. While accurate methods for protein quantification exist, the current assays involve sophisticated techniques, take long to administer and often require highly trained personnel for execution and analysis. Herein, we explore the development of a label-free biosensor for the detection and quantification of a standard protein. The developed biosensors comprise carbon nanotubes (CNTs), a specific antibody and cellulose filtration paper. The change in electrical resistance of the CNT-based biosensor system was used to sense a standard protein, bovine serum albumin (BSA) as a proof-of-concept. The developed biosensors were found to have a limit of detection of 2.89 ng/mL, which is comparable to the performance of the typical ELISA method for BSA quantification. Additionally, the newly developed method takes no longer than 10 min to perform, greatly reducing the time of analysis compared to the traditional ELISA technique. Overall, we present a versatile, affordable, simplified and rapid biosensor device capable of providing great benefit to both biological research and clinical diagnostics.

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Section: Analysis and Comparison With Elisa And Recently Developed Mementioning

confidence: 98%

Carbon Nanotube-Based Electrochemical Biosensor for Label-Free Protein Detection

et al. 2019

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“…EIS is an electroanalytical method to monitor electrochemical changes formed on the electrode surface [35,36]. Fig.…”

Section: Electrochemical Characterization Of the Bioelectrodementioning

confidence: 99%

A new, sensitive and disposable electrochemical immunosensor based on Benzaldehyde side group containing phosphazene polymer modified ITO substrate for Interleukin 1β detection

Aydın

2019

Hacettepe Journal of Biology and Chemistry

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B u çalışmada, interlökin 1β (IL 1β) tespiti için tek kullanımlık benzaldehit ikameli fosfazen polimeri (BSPP) ile modifiye edilmiş ITO elektrot temelli yeni bir elektrokimyasal ultrahassas immünosensör geliştirilmiştir. Aldehit yan grupları içeren fosfazen polimeri (BSPP) halka açma polimerizasyonu yöntemi ile sentezlenmiştir. Bu aldehit grupları, anti-IL 1β antikorları için bir bağlantı noktaları sağlamıştır. Önerilen immünosensörün üretim süreci, Elektrokimyasal Empedans Spektroskopisi (EIS) ve Döngüsel Voltammetri (CV) gibi elektrokimyasal tekniklerle izlendi. Ek olarak, bu üretim aşamaları, Taramalı Elektron Mikroskobu (FE-SEM) ve Atomik Kuvvet Mikroskobu (AFM) kullanılarak karakterize edildi. Ayrıca, polimer kaplı elektrot yüzeyindeki BSPP polimer tabakası, Enerji Dağıtıcı X-ışını (EDX) kullanılarak araştırıldı. Üretilen immünosensör düşük bir tespit sınırına (9.3 fg / mL) ve geniş bir doğrusal tayin aralığına (0.03-7.5 pg / mL) sahiptir. Ayrıca, iyi tekrarlanabilirlik (%1.82), mükemmel tekrarlanabilirlik (%1.56), iyi seçicilik ve yüksek stabiliteye sahipti. Deney sonuçları, BSPP polimerinin, klinik teşhis ve pratik uygulamalarda IL1β antijen tespiti için arzu edilen bir platform olduğunu gösterdi. Önerilen biyosensörün uygulanabilirliği, insan serumunda IL 1β seviyesi ölçülerek test edildi ve önerilen immünosensör, nicel analiz için kabul edilebilir sonuçlara sahipti. Anahtar Kelimeler Poli(fosfazen), label free immunosensor, electrokimyasal impedans spectroskopi, interleukin 1β (IL 1β).

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“…It is well known that EIS is an excellent technique for investigating the interface properties of surface-modified electrodes [44,45]. A typical impedance spectrum (presented in the form of the Nyquist plot) exhibits a semicircle near the origin at higher frequencies corresponding to the electron transfer-limited process and a linear tail at lower frequency range representing the diffusion-limited process.…”

Section: Eis Characterization Of the Dna Biosensormentioning

confidence: 99%

Label-free electrochemical DNA biosensor for rapid detection of mutidrug resistance gene based on Au nanoparticles/toluidine blue–graphene oxide nanocomposites

Peng

Liu

et al. 2015

Sensors and Actuators B: Chemical

146

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A sensitive and label-free electrochemical impedance biosensor for protein detection based on terminal protection of small molecule-linked DNA

Cited by 41 publications

References 30 publications

Carbon Nanotube-Based Electrochemical Biosensor for Label-Free Protein Detection

Carbon Nanotube-Based Electrochemical Biosensor for Label-Free Protein Detection

A new, sensitive and disposable electrochemical immunosensor based on Benzaldehyde side group containing phosphazene polymer modified ITO substrate for Interleukin 1β detection

Label-free electrochemical DNA biosensor for rapid detection of mutidrug resistance gene based on Au nanoparticles/toluidine blue–graphene oxide nanocomposites

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