A sensitive and label-free impedimetric biosensor based on an adjunct probe

Zhang, Xi Yuan; Zhou, Long Yin; Luo, Hong Qun; Li, Nian Bing

doi:10.1016/j.aca.2013.03.030

Cited by 17 publications

(7 citation statements)

References 43 publications

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“…Among the aforementioned detection techniques, electrochemical impedance spectroscopy (EIS) is gaining attention for DNA determination in solution due to its high sensitivity and label-free nature. , Here, the hybridization itself can be directly quantified in relation to the resistance offered to the electron transfer for the reduction/oxidation (redox) reaction of a mediator. The evolution of the redox reaction is then probed by monitoring the linearity of the voltage–current response in a range of frequencies and fitting the results using an equivalent circuit model .…”

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confidence: 99%

High-Performance Three-Dimensional Tubular Nanomembrane Sensor for DNA Detection

Medina‐Sánchez

Ibarlucea²,

Pérez

et al. 2016

Nano Lett.

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We report an ultrasensitive label-free DNA biosensor with fully on-chip integrated rolled-up nanomembrane electrodes. The hybridization of complementary DNA strands (avian influenza virus subtype H1N1) is selectively detected down to attomolar concentrations, an unprecedented level for miniaturized sensors without amplification. Impedimetric DNA detection with such a rolled-up biosensor shows 4 orders of magnitude sensitivity improvement over its planar counterpart. Furthermore, it is observed that the impedance response of the proposed device is contrary to the expected behavior due to its particular geometry. To further investigate this difference, a thorough model analysis of the measured signal and the electric field calculation is performed, revealing enhanced electron hopping/tunneling along the DNA chains due to an enriched electric field inside the tube. Likewise, conformational changes of DNA might also contribute to this effect. Accordingly, these highly integrated three-dimensional sensors provide a tool to study electrical properties of DNA under versatile experimental conditions and open a new avenue for novel biosensing applications (i.e., for protein, enzyme detection, or monitoring of cell behavior under in vivo like conditions).

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confidence: 99%

High-Performance Three-Dimensional Tubular Nanomembrane Sensor for DNA Detection

Medina‐Sánchez

Ibarlucea²,

Pérez

et al. 2016

Nano Lett.

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“…Zhang et al (2013b) used an adjunct probe for signal amplifi cation. An adjunct probe, thiol-modifi ed DNA sequence with 14 bases, functioned as a fi xer to immobilize the dissociative element of reporter probe to form loop structure.…”

Section: Aptamer-based Impedimetric Biosensorsmentioning

confidence: 99%

A review on impedimetric biosensors

Bahadır

Sezgintürk

2014

Artificial Cells, Nanomedicine, and Biotechnology

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131

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Electrochemical impedance spectroscopy (EIS) is a sensitive technique for the analysis of the interfacial properties related to biorecognition events such as reactions catalyzed by enzymes, biomolecular recognition events of specific binding proteins, lectins, receptors, nucleic acids, whole cells, antibodies or antibody-related substances, occurring at the modified surface. Many studies on impedimetric biosensors are focused on immunosensors and aptasensors. In impedimetric immunosensors, antibodies and antigens are bound each other and thus immunocomplex is formed and the electrode is coated with a blocking layer. As a result of that electron transfer resistance increases. In impedimetric aptasensors, impedance changes following the binding of target sequences, conformational changes, or DNA damages. Impedimetric biosensors allow direct detection of biomolecular recognition events without using enzyme labels. In this paper, impedimetric biosensors are reviewed and the most interesting ones are discussed.

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“…Electrochemical impedance spectroscopy (EIS), based on the response of an electrochemical system (cell) to applied potential, monitors sensitive changes in the conductivity/resistivity or the charging capacity of electrochemical interfaces. 55 It has become a powerful tool in studying biorecognition processes using modied electrodes with attached biological receptors on their surfaces (Fig. 9).…”

Section: Electrochemical Impedance Spectroscopymentioning

confidence: 99%