A sensitive and practical colorimetric test for polymer-supported hydroxyl and thiol groups

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

“…These strategies will be further discussed in the next section. In solid phase synthesis, color tests [314][315][316][317][318][319][320] are the equivalence of thin layer chromatography for solution phase organic synthesis. Once a resin undergoes a chemical transformation with a reagent, a small portion of the resin is treated with a dye that is highly reactive for the chemical function that has to be consumed in the actual reaction.…”

“…Low MW azide compounds can be seriously explosive [325,335] while low MW thiol compounds generally have a deterring smell. Moreover, there are color tests to detect remaining amounts of both azide [320] and thiol [314] groups. As mentioned in the previous section, azide, alkyne, alkene and thiol groups can be easily followed by IR spectroscopy which makes these reactions further attractive on solid phase.…”

Thiol-ene and thiol-yne chemistry in microfluidics: a straightforward method towards macroporous and nonporous functional polymer beads

“…In general, the lower loading in the beads can be attributed to the restricted access in the highly crosslinked system on one hand and to the leaching of the some fraction of monofunctional monomers to the continuous aqueous phase on the other hand. It is worth to note that the highly sensitive colorimetric thiol test 53 (with a detection limit as low as 5 µmol/g) was positive for all the beads, which indicates the presence of unreacted free thiols. We further quantified free thiol groups of various functional beads via the Ellman's colorimetric test.…”

“…The hydroxyl loading was calculated using the Lambert's Beer Law. The beads were also subjected to a simple colorimetric test 53 and Ellman's colorimetric test 54 for qualitative detection and quantitative estimation of unreacted thiols.…”

Colorimetric Test for Solid‐Phase Organic Synthesis

“…16 In the presence of DMAP, alcohols, phenols, and primary thiols can also be detected. 17 Detection limit of 3.4 mmol/g for aniline groups 16 and 3-5 mmol/g for NF31/DMAP test. 17 Other Reagents: 2,6-Di-tert-butylpyridine and trifluoroacetic acid.…”