A sensitive and selective quantification of catecholamine neurotransmitters in rat microdialysates by pre-column dansyl chloride derivatization using liquid chromatography–tandem mass spectrometry

Nirogi, Ramakrishna; Komarneni, Prashanth; Kandikere, Vishwottam; Boggavarapu, Rajeshkumar; Bhyrapuneni, Gopinadh; Benade, Vijay; Gorentla, Srinivasarao

doi:10.1016/j.jchromb.2012.09.034

Cited by 47 publications

(21 citation statements)

References 27 publications

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“…Samples and dopamine standards were pre-processed by dansyl chloride derivatization based on the method of ref. [34], as described (SI). Quantification was performed by the Biomarker Core at the Irving Center for Clinical and Translational Research at Columbia University Medical Center as described (SI).…”

Section: Microdialysis Samplingmentioning

confidence: 99%

Striatal dopamine D2 receptors regulate effort but not value-based decision making and alter the dopaminergic encoding of cost

Filla

Bailey

Schipani

et al. 2018

Neuropsychopharmacol

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Deficits in goal-directed motivation represent a debilitating symptom for many patients with schizophrenia. Impairments in motivation can arise from deficits in processing information about effort and or value, disrupting effective cost-benefit decision making. We have previously shown that upregulated dopamine D2 receptor expression within the striatum (D2R-OE mice) decreases goal-directed motivation. Here, we determine the behavioral and neurochemical mechanisms behind this deficit. Female D2R-OE mice were tested in several behavioral paradigms including recently developed tasks that independently assess the impact of Value or Effort manipulations on cost-benefit decision making. In vivo microdialysis was used to measure extracellular dopamine in the striatum during behavior. In a value-based choice task, D2R-OE mice show normal sensitivity to changes in reward value and used reward value to guide their actions. In an effort-based choice task, D2R-OE mice evaluate the cost of increasing the number of responses greater relative to the effort cost of longer duration responses compared to controls. This shift away from choosing to repeatedly execute a response is accompanied by a dampening of extracellular dopamine in the striatum during goal-directed behavior. In the ventral striatum, extracellular dopamine level negatively correlates with response cost in controls, but this relationship is lost in D2R-OE mice. These results show that D2R signaling in the striatum, as observed in some patients with schizophrenia, alters the relationship between effort expenditure and extracellular dopamine. This dysregulation produces motivation deficits that are specific to effort but not value-based decision making, paralleling the effort-based motivational deficits observed in schizophrenia.

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Section: Microdialysis Samplingmentioning

confidence: 99%

Striatal dopamine D2 receptors regulate effort but not value-based decision making and alter the dopaminergic encoding of cost

Filla

Bailey

Schipani

et al. 2018

Neuropsychopharmacol

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“…Tonic and burst firing neurons release Dopamine (DA) that can then diffuse and act predominantly at remote extra-synaptic receptors before reuptake by DA transporters. As a result, target neurons are tonically exposed to DA; e.g., approximately tens of nM in the striatum and prefrontal cortex (Owesson-White et al, 2012; Nirogi et al, 2013; Zuo et al, 2013), and superimposed upon this baseline are periodic fluctuations in DA that can transiently rise to ~µM levels near the release sites of bursting DA neurons (Park et al, 2011; Rice et al, 2011; Owesson-White et al, 2012). …”

Section: Introductionmentioning

confidence: 99%

Dopaminergic tone persistently regulates voltage-gated ion current densities through the D1R-PKA axis, RNA polymerase II transcription, RNAi, mTORC1, and translation

Krenz¹,

Parker²,

Rodgers³

et al. 2014

Front. Cell. Neurosci.

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Long-term intrinsic and synaptic plasticity must be coordinated to ensure stability and flexibility in neuronal circuits. Coordination might be achieved through shared transduction components. Dopamine (DA) is a well-established participant in many forms of long-term synaptic plasticity. Recent work indicates that DA is also involved in both activity-dependent and -independent forms of long-term intrinsic plasticity. We previously examined DA-enabled long-term intrinsic plasticity in a single identified neuron. The lateral pyloric (LP) neuron is a component of the pyloric network in the crustacean stomatogastric nervous system (STNS). LP expresses type 1 DA receptors (D1Rs). A 1 h bath application of 5 nM DA followed by washout produced a significant increase in the maximal conductance (Gmax) of the LP transient potassium current (IA) that peaked ~4 h after the start of DA application; furthermore, if a change in neuronal activity accompanied the DA application, then a persistent increase in the LP hyperpolarization activated current (Ih) was also observed. Here, we repeated these experiments with pharmacological and peptide inhibitors to determine the cellular processes and signaling proteins involved. We discovered that the persistent, DA-induced activity-independent (IA) and activity-dependent (Ih) changes in ionic conductances depended upon many of the same elements that enable long-term synaptic plasticity, including: the D1R-protein kinase A (PKA) axis, RNA polymerase II transcription, RNA interference (RNAi), and mechanistic target of rapamycin (mTOR)-dependent translation. We interpret the data to mean that increasing the tonic DA concentration enhances expression of a microRNA(s) (miRs), resulting in increased cap-dependent translation of an unidentified protein(s).

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“…Dansyl chloride is a reagent commonly used for the derivatization of amines and nitrogen or hydroxyl‐containing compounds (Tang and Guengerich, ; Cai et al ., ; Lamshöft et al ., ). Several dansyl derivatization conditions have been published using different buffers to set basic medium, solvents, time and temperature derivatization (Noestheden et al ., ; Chang et al ., ; Nirogi et al ., ; Huang et al ., ). Our conditions were based on a previous assay used in the laboratory with bicarbonate buffer and acetone as solvent.…”

Section: Resultsmentioning

confidence: 99%

Quantification of β‐aminopropionitrile, an inhibitor of lysyl oxidase activity, in plasma and tumor of mice by liquid chromatography tandem mass spectrometry

Machon

Calvé

Coste

et al. 2014

Biomedical Chromatography

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Lysyl oxidase enzymes are reported to be involved in patho-physiological process such as tumorigenesis. β-Aminopropionitrile (BAPN) is an irreversible inhibitor of lysyl oxidase activity, suggesting a potentially useful therapeutic of interest in oncology. This paper describes the first assay concerning the quantification of BAPN by mass spectrometry. A high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) assay was developed for the quantification of BAPN in plasma and tumor of mice. This method combines dansyl chloride (Dns) derivatization and extraction using a solid-phase extraction Oasis Max column. Deuterated BAPN was used as internal standard (IS). Separation was achieved using an C₁₈ column HypersylGold, (ThermoElectron), 3.0 µm (100 × 2.1 mm i.d.). Gradient elution with water containing 0.1% acetic acid (A) and acetonitrile containing 0.1% acetic acid (B) was applied. Detection was performed with an electrospray ionization interface operating in negative ion mode. Selected reaction monitoring was used with ion transitions m/z 302 → 249 for BAPN-Dns and m/z 306 → 250 for the IS. The method was fully validated in plasma and was linear and sensitive in the range of 10-500 ng/mL. The lower limit of quantification in plasma was 2.5 ng/mL. This validated assay was successfully applied to a kinetic study of BAPN in mouse plasma and demonstrates that BAPN reaches the tumoral tissue.

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A sensitive and selective quantification of catecholamine neurotransmitters in rat microdialysates by pre-column dansyl chloride derivatization using liquid chromatography–tandem mass spectrometry

Cited by 47 publications

References 27 publications

Striatal dopamine D2 receptors regulate effort but not value-based decision making and alter the dopaminergic encoding of cost

Striatal dopamine D2 receptors regulate effort but not value-based decision making and alter the dopaminergic encoding of cost

Dopaminergic tone persistently regulates voltage-gated ion current densities through the D1R-PKA axis, RNA polymerase II transcription, RNAi, mTORC1, and translation

Quantification of β‐aminopropionitrile, an inhibitor of lysyl oxidase activity, in plasma and tumor of mice by liquid chromatography tandem mass spectrometry

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