A sensitive and specific ELISA using a monoclonal capture antibody for detection of Tamm-Horsfall urinary glycoprotein in serum

Urinary Tamm‐Horsfall Glycoprotein Concentrations in Normal and Urolithiasis‐Affected Male Cats Determined by an ELISA

“…Precision was improved by weighted log-logit transformations of the data (TIJSSEN, 1985). The reproducibility of cTHP readings in the linear portion of three standard curves (mean CV = 3.6 %) compared favorably with the CV of 5.9 f 2.9 YO reported for a hTHP ELISA (HUNT et al, 1986).…”

“…The purification procedure yielded cTHP which probably was as pure as most hTHP isolates, where purity was judged by much less sensitive Coomassie blue staining of gels (DAWNAY et al, 1980;KURIYAMA and SILVERBLATT, 1986;WIGGENS et al, 1986) or where hTHP was "the major protein" observed on SDS-PAGE gels (HUNT et al, 1985;HUNT et al, 1986). It is not known whether the minor contaminating bands were unique proteins or were proteolytic fragments of cTHP.…”

Uromodulin Biology and Pathophysiology – An Update

“…Both indirect as well as direct ELISA setups have been reported for urinary [5,[97][98][99][100][101] and serum [91,102,103] measurements. In the 1990s, Syn elisa THP immunoassay was available from Elias Medizintechnik GmbH (Freiburg, Germany; which later became Elias/Pharmacia & Upjohn), but its production was eventually discontinued.…”

Goat uromodulin promoter directs kidney-specific expression of GFP gene in transgenic mice

“…It has been reported that small quantities of uromodulin/THP can be detected in blood using sensitive immunoassays [29,30], but it is possible that some proteins in the serum simply cross-react with the antibody raised against the uromodulin/THP proteins [31]. The renal environment appears not to be essential for transcription of the uromodulin/THP gene or synthesis of the protein.…”