A Sensitive Assay for ZYAN1 in Human Whole Blood and Urine Utilizing Positive LC–MS/MS Electrospray Ionization

Patel, Harilal; Soni, Krunal; Trivedi, Rucha; Heading, Heather; Geue, Jason; Kansagra, Kevinkumar; Gupta, Rahul; Pandya, Vrajesh B.; Srinivas, Nuggehally R.; Patel, Pankaj; Desai, Ranjit C.

doi:10.4155/bio-2017-0014

Cited by 2 publications

(1 citation statement)

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“…In contrast to roxadustat, daprodustat, and molidustat, little has been published about the metabolic fate of other HIF–prolyl hydroxylase inhibitors such as desidustat, enarodustat, vadadustat, and TP0463518 (Figure , 8 – 11 ). All of these compounds reportedly support maintaining and/or increasing hemoglobin levels in preclinical and clinical trials − and, consequently, necessitate monitoring in the context of doping controls, which currently relies on targeting the intact drugs (as, e.g., demonstrated for desidustat) and in silico predicted metabolic products. The mimicking of N -oxalylglycine by these drug candidates is accommodated by glycineamide side chains at the respective pharmacophores, all of which exhibit a hydroxyl function available for glucuronidation similar to roxadustat.…”

Section: The Old the New And The Difficult (Analytes)mentioning

confidence: 99%

Analytical Approaches in Human Sports Drug Testing: Recent Advances, Challenges, and Solutions

2019

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Section: The Old the New And The Difficult (Analytes)mentioning

confidence: 99%

Analytical Approaches in Human Sports Drug Testing: Recent Advances, Challenges, and Solutions

2019

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A Novel Spectrophotometric Method Development for Quantification of Desidustat in Bulk and Pharmaceutical Dosage Form

Sharma,

Jain

2023

DMBL

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Background: Desidustat (DES) belongs to a new category of drugs, i.e., Hypoxia-Inducible Factor (HIF) propyl hydroxylase inhibitor, and is used for the treatment of anemia in chronic kidney disease. However, no method has yet been reported in the literature for the estimation of drugs. Objective: The objective of the study is to develop a simple, precise, and accurate method for determining DES in bulk and pharmaceutical dose form. Methods: The stability-indicating HPLC method for assay included the use of Kromasil 100-5-C8 (100 mm × 4.6 mm) column, UV detector 224 nm, mobile phase composition involving a mixture of acetonitrile:water (52:48), and a flow rate of 1.0 mL/min. ICH guidelines were followed for the method's validation. To assess the method's specificity and stability in showing characteristics, stress degradation studies were carried out. The working standard solution of dapagliflozin was exposed to 1 and 2 N HCl by refluxing 1 and 2 N NaOH with 30% hydrogen peroxide by volume and UV radiation in order to conduct a degradation study. Results: Maximum absorbance wavelength was observed at 229 nm. The sample solution remained stable for up to 12 hours. The linear response from 2 to 12 μg/ml of DES was y = 0.1087x + 0.0962 and r2 = 0.9963. The accuracy was between 100 to 101%. Precision was recorded under three criteria: repeatability, intraday and interday, for which results fell within the acceptable ranges (<2%). The limit of detection (LOD) and limit of quantification (LOQ) of the technique were 0.434 μg/ml and 1.316 μg/ml, respectively. Conclusion: The proposed method was found to be beneficial for drug monitoring and the ongoing analysis of DES in research and quality control laboratories. This approach is simple, precise, rapid, economical, and sensitive

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A Sensitive Assay for ZYAN1 in Human Whole Blood and Urine Utilizing Positive LC–MS/MS Electrospray Ionization

Abstract: The validated assay was applied for clinical pharmacokinetics of ZYAN1 in healthy volunteers.

Cited by 2 publications

References 9 publications

Analytical Approaches in Human Sports Drug Testing: Recent Advances, Challenges, and Solutions

Analytical Approaches in Human Sports Drug Testing: Recent Advances, Challenges, and Solutions

A Novel Spectrophotometric Method Development for Quantification of Desidustat in Bulk and Pharmaceutical Dosage Form

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