A sensitive biomimetic enzyme-linked immunoassay method based on Au@Pt@Au composite nanozyme label and molecularly imprinted biomimetic antibody for histamine detection

Chen, Yongfeng; Yu, Runze; Wang, Rui-Qiang; Zhou, Xu

doi:10.1080/09540105.2021.1978945

Cited by 9 publications

(5 citation statements)

References 52 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The histamine dihydrochloride, tryptamine, and the MIP before elution showed absorption peaks at 1025, 1022, and 1029 cm −1 that were assigned to the C-N stretching vibrations of histamine and tryptamine. The small difference in the C-N absorption peak position in the MIP (before elution) was likely due to the amino of template molecules and the carbonyl of MAA forming hydrogen bonds [ 16 ]. After the elution step, the C-N peak disappeared, indicating the template molecules (histamine dihydrochloride and tryptamine) had been successfully eluted from the MIP.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Development of an Immunoassay Method for the Sensitive Detection of Histamine and Tryptamine in Foods Based on a CuO@Au Nanoenzyme Label and Molecularly Imprinted Biomimetic Antibody

et al. 2022

Self Cite

View full text Add to dashboard Cite

In this paper, a novel biomimetic enzyme-linked immunoassay method (BELISA) was successfully established for the detection of histamine and tryptamine, based on catalytically active cupric oxide@gold nanoparticles (CuO@Au NPs) as a marker and a molecularly imprinted polymer (MIP) as the biomimetic antibody. Under optimized conditions, the detection limitations of the BELISA method for histamine and tryptamine were 0.04 mg L−1 and 0.14 mg L−1, respectively. For liquor spiked with histamine and tryptamine, the BELISA method delivered satisfactory recoveries ranging from 89.90% to 115.00%. Furthermore, the levels of histamine and tryptamine in fish, soy sauce, and rice vinegar samples were detected by the BELISA method and a high performance liquid chromatography method, with no significant difference between the two methods being found. Although the catalytic activity of nanozymes is still lower than that of natural enzymes, the BELISA method could still sensitively determine the histamine and tryptamine levels in food samples.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Natural enzymes are large bulky macromolecules and thus introduce shielding effects, accordingly interfering with marker recognition by the MIPs [ 14 ]. Therefore, it is necessary to find new markers with simple structures to replace natural enzymes in BELISA [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Development of an Immunoassay Method for the Sensitive Detection of Histamine and Tryptamine in Foods Based on a CuO@Au Nanoenzyme Label and Molecularly Imprinted Biomimetic Antibody

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, the Nyquist plot recorded a semi-circle that could be utilized in charge-transfer resistance ( R ct ) estimations. 40 In this study, differential pulse voltammetry (DPV) was also conducted due to it is selectivity and sensitivity over CV. 31 The scanning potentials employed during the DPV ranged from −0.1 to +0.9 V.…”

Section: Methodsmentioning

confidence: 99%

“…The interface or the specific sections of the impedance was addressed as resistance or Z ′, and the imaginary part or Z ″. Furthermore, the Nyquist plot recorded a semi-circle that could be utilized in charge-transfer resistance ( R ct ) estimations . In this study, differential pulse voltammetry (DPV) was also conducted due to it is selectivity and sensitivity over CV .…”

Section: Methodsmentioning

confidence: 99%

“…Wang et al 40 Ahmed et al 25 Mahnashi et al 41 Fan et al 42 Peng et al 43 The determination of histamine using MIP/SPE/PU/ LiClO 4 using the voltammetric approach presents a satisfactory outcome. Generally, voltammetry relies on the development of current signal and charge upon histamine as the analyte and MIP as the receptor binder.…”

Section: Table 1 Comparison Of the Analytical Performance Of Detectio...mentioning

confidence: 99%

See 1 more Smart Citation

Inspecting Histamine Isolated from Fish through a Highly Selective Molecularly Imprinted Electrochemical Sensor Approach

Munir¹,

Rahmawati

Jamal

et al. 2023

ACS Omega

View full text Add to dashboard Cite

Numerous analytical approaches have been developed to determine histamine levels in food samples due to its health consequences. Consuming histamine over the Food and Drug Administration (FDA)-regulated 50 mg kg −1 limit would result in chronic toxicity. Consequently, the present study discusses a novel electrochemical approach to evaluate histamine levels in fish products via a molecularly imprinted polymer (MIP) on an electrode surface. The film was produced with electropolymerized polyurethane (PU), which maintained the histamine compound. Fourier-transform infrared (FTIR) spectroscopy was applied to verify the MIP manufactured in this study. The capability of the polymer was measured by assessing its electron shifts with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Differential pulse voltammetry (DPV) was also employed to validate the sensing method. The MIP/ screen-printed electrode (SPE) and non-imprinted polymer (NIP)/SPE recorded a linear response ranging from 1 to 1000 nmol L −1 at the 1.765 and 709 nmol L −1 detection limits. The sensing technique was subsequently utilized to determine the histamine levels in selected samples at room temperature (25 °C). Generally, the sensor allowed the accurate and precise detection of histamine in the fish samples. Furthermore, the approach could be categorized as a simple technique that is low-cost and suitable for on-site detections.

show abstract

Direct competitive immunoassay method for sensitive detection of the histamine in foods based on a MI‐Cu‐GMP nanozyme marker and molecularly imprinted biomimetic antibody

Peng,

Wang,

et al. 2024

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUNDHistamine may lead to low blood pressure, skin flushing and edema when it accumulates in large amounts in the body. Therefore, establishing sensitive methods for the detection of histamine in foods is extremely important to ensure food safety and human health.RESULTSThe MI‐Cu‐GMP NPs (2‐methylimidazole‐copper‐guanosine monophosphate nanozymes) with high laccase‐like activity were synthesized. Using the prepared molecular imprinted membrane as biomimetic antibody and MI‐Cu‐GMP NPs as marker, a sensitive direct competitive biomimetic enzyme‐linked immunoassay (BELISA) method for rapid detection of the histamine in foods was developed. Under optimal conditions, the limit of detection (LOD, IC15) and sensitivity (IC50) of the BELISA method for histamine was 0.05 mg L−1 and 1.22 mg L−1, respectively. The liquor samples spiked with histamine was detected by the BELISA method with satisfactory recoveries ranging from 90.00% to 116.00%. Further, the level of histamine in three samples (cooking wine, rice vinegar and soy sauce) was tested by the BELISA and high‐performance liquid chromatography (HPLC), with no significant difference found between the two methods.CONCLUSIONGiven the advantages, the established BELISA method is expected to provide practical guidance for the monitoring of histamine in food and provides a foundation for the detection of other food hazards. © 2024 Society of Chemical Industry.

show abstract

A sensitive biomimetic enzyme-linked immunoassay method based on Au@Pt@Au composite nanozyme label and molecularly imprinted biomimetic antibody for histamine detection

Abstract: Xu (2021) A sensitive biomimetic enzyme-linked immunoassay method based on Au@Pt@Au composite nanozyme label and molecularly imprinted biomimetic antibody for histamine detection,

Cited by 9 publications

References 52 publications

Development of an Immunoassay Method for the Sensitive Detection of Histamine and Tryptamine in Foods Based on a CuO@Au Nanoenzyme Label and Molecularly Imprinted Biomimetic Antibody

Development of an Immunoassay Method for the Sensitive Detection of Histamine and Tryptamine in Foods Based on a CuO@Au Nanoenzyme Label and Molecularly Imprinted Biomimetic Antibody

Inspecting Histamine Isolated from Fish through a Highly Selective Molecularly Imprinted Electrochemical Sensor Approach

Direct competitive immunoassay method for sensitive detection of the histamine in foods based on a MI‐Cu‐GMP nanozyme marker and molecularly imprinted biomimetic antibody

Contact Info

Product

Resources

About