A sensitive biosensor for determination of pathogenic bacteria using aldehyde dehydrogenase signaling system

Zhang, Wenguang; Bu, Shengjun; Bai, Huasong; Ma, Cambridge; Ma, Li; Wei, Hongguo; Liu, Xiu; Li, Zehong; Wan, Jiayu

doi:10.1007/s00216-020-02928-7

Cited by 4 publications

(1 citation statement)

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“…Initially, the linker probe was locked through the S. typhimurium aptamer. Aptamers are single-stranded oligonucleotide sequences for target molecules and can efficiently and specifically bind to target molecules [ 22 , 23 ]. The aptamer was shed, and HCR-binding sites were exposed.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical biosensor for detecting pathogenic bacteria based on a hybridization chain reaction and CRISPR-Cas12a

Liu

Feng

et al. 2021

Anal Bioanal Chem

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In this study, Lba Cas12a (Cpf1) as one of the CRISPR systems from Lachnospiraceae bacterium was coupled with a hybridization chain reaction (HCR) to develop an electrochemical biosensor for detecting the pathogenic bacterium, Salmonella typhimurium . Autonomous cross-opening of functional DNA hairpin structures of HCR yielded polymer double-stranded DNA wires consisting of numerous single-stranded DNAs, which initiated the trans-cleavage activity of CRISPR-Cas12a to indiscriminately cleave random single-stranded DNA labeling electrochemical tags on the surface of the electrode. It led to a variation in the electron transfer of electrochemical tags. The polymer double-stranded DNA of HCR was immobilized on dynabeads (DBs) via the S. typhimurium aptamer and released from DBs. The established method could selectively and sensitively quantify S. typhimurium in samples with detection limits of 20 CFU/mL. Our study provides a novel insight for exploring universal analytical methods for pathogenic bacteria based on CRISPR-Cas12a coupled with HCR. Supplementary Information The online version contains supplementary material available at 10.1007/s00216-021-03733-6.

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Section: Introductionmentioning

confidence: 99%