A sensitive cholesterol electrochemical biosensor based on biomimetic cerasome and graphene quantum dots

Wu, Shuyao; Jiang, Meijiao; Mao, Hui; Zhao, Nan; He, Dongqing; Chen, Qinan; Liu, Daliang; Zhang, Wei; Song, Xi‐Ming

doi:10.1007/s00216-022-03986-9

Cited by 21 publications

(16 citation statements)

References 41 publications

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“…[42] There are many reports where researchers have employed glassy carbon electrodes (GCE) as a sensing platform. [39,40,[43][44][45][46][47]50] Glassy carbon electrodes exhibit several drawbacks when compared to pencil graphite electrodes. Firstly, they are expensive, which can restrict their cost-effectiveness for certain applications.…”

Section: Demonstration Of Proof-of-conceptmentioning

confidence: 99%

Cost‐Effective Cholesterol Monitoring by Enzyme‐Based Electrochemical Assay

Eksin,

Senturk,

Erdem

2024

ChemistrySelect

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Herein we present a practical, disposable, enzyme‐based electrochemical biosensor to revolutionize a rapid and precise measurement of cholesterol level in saliva. To create a biosensor that can measure cholesterol levels, cholesterol oxidase (ChOx) was immobilized on disposable pencil graphite electrode (PGE) via EDC/NHS chemistry. This ChOx‐PGE biosensor has been characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS). Various critical experimental parameters, including nafion concentration, enzyme concentration, enzyme‐cholesterol interaction time, were optimized ensuring consistency and precision in our results and maintained constant throughout the experiments. Under optimum conditions, the detection limit was found to be 62 μM and the sensitivity of the sensor was calculated as 1112.8 Ohm mM−1 cm−2. The potential interferences such as glucose, ascorbic acid, uric acid, cysteine was selected to test the selectivity and no significant response was observed. Finally, the proposed biosensor was effectively employed to measure cholesterol level in saliva. This biosensor boasts a rapid response time of just 20 minutes and achieves a remarkable detection limit of 95 μM for salivary cholesterol, surpassing existing techniques. Our assay not only offers an innovative solution for cholesterol monitoring but also opens new horizons in point‐of‐care diagnostics with its disposable and practical design.

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Section: Demonstration Of Proof-of-conceptmentioning

confidence: 99%

Cost‐Effective Cholesterol Monitoring by Enzyme‐Based Electrochemical Assay

Eksin,

Senturk,

Erdem

2024

ChemistrySelect

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“…Subsequently, real sample analysis was performed with a pharmacological product Adrenalina WZF samples. Similarly, by using GRQDs, ceramic-coated liposomes (creasome, CRE), and ChOx, an electrochemical biosensor for cholesterol was fabricated [ 109 ]. Here, a layer-by-layer strategy was followed for the fabrication.…”

Section: Various Nanomaterial-modified Electrodesmentioning

confidence: 99%

Recent Developments in the Design and Fabrication of Electrochemical Biosensors Using Functional Materials and Molecules

Theyagarajan

Kim

2023

Biosensors

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Electrochemical biosensors are superior technologies that are used to detect or sense biologically and environmentally significant analytes in a laboratory environment, or even in the form of portable handheld or wearable electronics. Recently, imprinted and implantable biosensors are emerging as point-of-care devices, which monitor the target analytes in a continuous environment and alert the intended users to anomalies. The stability and performance of the developed biosensor depend on the nature and properties of the electrode material or the platform on which the biosensor is constructed. Therefore, the biosensor platform plays an integral role in the effectiveness of the developed biosensor. Enormous effort has been dedicated to the rational design of the electrode material and to fabrication strategies for improving the performance of developed biosensors. Every year, in the search for multifarious electrode materials, thousands of new biosensor platforms are reported. Moreover, in order to construct an effectual biosensor, the researcher should familiarize themself with the sensible strategies behind electrode fabrication. Thus, we intend to shed light on various strategies and methodologies utilized in the design and fabrication of electrochemical biosensors that facilitate sensitive and selective detection of significant analytes. Furthermore, this review highlights the advantages of various electrode materials and the correlation between immobilized biomolecules and modified surfaces.

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“…Furthermore, a high recovery rate in bread crust showed the potential for practical uses of technology. Wu et al were used GQDs and developed a straightforward and sensitive electrochemical cholesterol biosensor with high conductivity [41]. GQDs facilitates electron transport between the enzyme and the electrode more efficiently.…”

Section: Carbon-based Nanomaterialsmentioning

confidence: 99%

“…Liposome is a kind of biomimetic material with cell membrane structure, that has great biocompatibility with biomolecules. The membrane property of liposome can be used to modify the sensors, that is more beneficial to the combination of other substances with the sensors [41]. A cholesterol biosensor based on biomimetic liposomes and GQD was developed by Wu et al [41].…”

Section: Organic-based Nanomaterialsmentioning

confidence: 99%

“…The membrane property of liposome can be used to modify the sensors, that is more beneficial to the combination of other substances with the sensors [41]. A cholesterol biosensor based on biomimetic liposomes and GQD was developed by Wu et al [41]. The liposome used in the experiment has a ceramic soft interface and a lipid bilayer membrane, and the electrode surface is immobilized by layer-by-layer self-assembly method, that can serve great biocompatibility and uphold cholesterol oxidase activity.…”

Section: Organic-based Nanomaterialsmentioning

confidence: 99%

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Optically Active Nanomaterials and Its Biosensing Applications—A Review

et al. 2023

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This article discusses optically active nanomaterials and their optical biosensing applications. In addition to enhancing their sensitivity, these nanomaterials also increase their biocompatibility. For this reason, nanomaterials, particularly those based on their chemical compositions, such as carbon-based nanomaterials, inorganic-based nanomaterials, organic-based nanomaterials, and composite-based nanomaterials for biosensing applications are investigated thoroughly. These nanomaterials are used extensively in the field of fiber optic biosensing to improve response time, detection limit, and nature of specificity. Consequently, this article describes contemporary and application-based research that will be of great use to researchers in the nanomaterial-based optical sensing field. The difficulties encountered during the synthesis, characterization, and application of nanomaterials are also enumerated, and their future prospects are outlined for the reader’s benefit.

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A sensitive cholesterol electrochemical biosensor based on biomimetic cerasome and graphene quantum dots

Cited by 21 publications

References 41 publications

Cost‐Effective Cholesterol Monitoring by Enzyme‐Based Electrochemical Assay

Cost‐Effective Cholesterol Monitoring by Enzyme‐Based Electrochemical Assay

Recent Developments in the Design and Fabrication of Electrochemical Biosensors Using Functional Materials and Molecules

Optically Active Nanomaterials and Its Biosensing Applications—A Review

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