“…Among the antigens utilized in the DTH, from the published literature, are: tetanus toxoid, Candida albicans, Mycobacterium bovis [Bacillus CalmettGuerin (BCG)], SRBC, bovine serum albumin (BSA), KLH, and ovalbumin (OVA) (Lagrange and Mackaness, 1975;Vos, 1980;Whittingham et al, 1982;Henningsen et al, 1984;Hurtrel et al, 1984;Exon et al, 1990;Nghiem et al, 2002). Unfortunately, many of these antigens, such as SRBC, BSA, OVA, and KLH, are also capable of initiating a robust humoral immune response, and the negating effects of antigen-specific antibody (Ab) production on the DTH response are well-documented (Mackaness et al, 1974;Morikawa et al, 1991).…”