A sensitive electrochemical sensor for in vitro detection of parathyroid hormone based on a MoS2-graphene composite

Abstract

This paper reports a biosensor based on a MoS2-graphene (MG) composite that can measure the parathyroid hormone (PTH) concentration in serum samples from patients. The interaction between PTH and MG was analysed via an electrochemical sensing technique. The MG was functionalized using l-cysteine. Following this, PTH could be covalently immobilized on the MG sensing electrode. The properties of MG were evaluated using scanning electron microscopy, high-resolution transmission electron microscopy, X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectrometry. Following optimization of immobilized materials—such as MG, PTH, and alkaline phosphatase (ALP)—the performance of the MG sensor was investigated via cyclic voltammetry, to assess its linearity, repeatability, and reproducibility. Electrochemical impedance spectroscopy was performed on graphene oxide (GO) and MG-modified electrodes to confirm the capture of a monoclonal antibody (MAb) targeting PTH. Furthermore, the ALP-PTH-MG sensor exhibits a linear response towards PTH from artificial serum over a range of 1–50 pg mL−1. Moreover, patient sera (n = 30) were evaluated using the ALP-PTH-MG sensor and compared using standard equipment (Roche E 170). The P-value is less than 0.01 when evaluated with a t-test using Welch’s correction. This implies that the fabricated sensor can be deployed for medical diagnosis.

Nucleic aptamer modified porous reduced graphene oxide/MoS2 based electrodes for viral detection: Application to human papillomavirus (HPV)

“…The extraordinary properties of layered prGO for sensing have renewed the interest to investigate other 2D materials such as the semiconducting analog of graphene, molybdenum disulfide (MoS 2 ) [28][29][30]. As both MoS 2 and rGO have similar morphology and layered structure, the formulation of MoS 2 -rGO hybrids has shown to maximize their structural compatibility and resulted in improved electrochemical properties [31][32][33][34][35]. We showed indeed that a MoS 2 -rGO modified electrode allowed for the selective analysis of folic acid in the presence of a variety of interference species with a 10 nM detection limit and applicable for the determination of folic acid in human serum [35].…”

An ultrasensitive electrochemical immunosensor platform based on disposableITO electrode modified by 3-CPTMS for early detection of parathyroid hormone

“…The interaction between PTH and MG was analyzed via an electrochemical sensing technique. Furthermore, the ALP-PTH-MG sensor exhibits a linear response towards PTH from artificial serum over a range of 1-50 pg mL [20]. Dittmer et al described a rapid method for the sensitive detection of proteins using actuated magnetic particle labels, which are measured with a giant magnetoresistive biosensor.…”

Membrane-Based Microwave-Mediated Electrochemical Immunoassay for the In Vitro, Highly Sensitive Detection of Osteoporosis-Related Biomarkers

“…Electrochemical immunoassays have been studied in many ways to increase sensitivity, cost effectiveness, efficiencies in pretreatment, amplification, and simple handling [ 13 , 14 , 15 , 16 ]. Recently, the electrochemical immunoassay of osteoporosis has been studied regarding the sensing electrode carrying gold nanoparticles [ 17 ] or MoS 2 -graphene composite [ 18 ]. In this study, we developed a simple electrochemical immunoassay based on the microwave-mediated immobilization of antibodies and the antibody–antigen reaction.…”