A sensitive enzyme-linked immunosorbent assay for human interleukin-8

Ko, Y C; Mukaida, Naofumi; Panyutich, Alexander V.; Voitenok, Nikolai N.; Matsushima, Kouji; Kawai, Tadashi; Kimura, Tadashi

doi:10.1016/0022-1759(92)90254-q

Cited by 108 publications

(66 citation statements)

References 18 publications

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“…The concentration of MCP-1 in the collected media from each well was determined by ELISA as described previously. 25 Briefly, each well of 96-well microtiter plate was coated with monoclonal anti-human MCP-1 antibody (ME61;1 mg/ml) overnight at 41C. After washing, the plates were blocked by incubation with phosphatebuffered saline (PBS) containing 1% bovine serum albumin for 1 hour at 371C.…”

Section: Enzme-linked Immunosorbent Assay (Elisa) For Mcp-1mentioning

confidence: 99%

Enhanced antitumor effects of a bicistronic adenovirus vector expressing both herpes simplex virus thymidine kinase and monocyte chemoattractant protein-1 against hepatocellular carcinoma

et al. 2003

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The efficacy of the suicide gene therapy using the herpes simplex virus thymidine kinase/ganciclovir (HSV-tk/GCV) system for the treatment of cancer is limited because of the insufficient gene transfer and the low killing activity. To enhance the antitumor activity, we determined whether recombinant adenovirus vector (rAd)s expressing both HSV-tk and monocyte chemoattractant protein-1 (MCP-1) genes could potentiate the destruction of hepatocellular carcinoma (HCC). The rAd Ad-tk-MCP1 harboring HSVtk and MCP-1 genes in sequence under the universal CAG promoter was constructed with a bicistronic unit including the encephalomyocarditis virus-internal ribosomal entry site. The levels of HSV-tk expression and GCV-sensitive tumoricidal activity of Ad-tk-MCP1 were comparable to those of rAd expressing HSV-tk alone. The growth of subcutaneous tumors in athymic nude mice was markedly suppressed when tumors were treated with Ad-tk-MCP1 as opposed to another bicistronic vector Ad-MCP1-tk, rAd expressing either HSV-tk or MCP-1, or both of these vectors. The antitumor effects of Ad-tkMCP1 may be dependent on the activation of macrophages, since the recruitment of macrophages was observed tumor necrosis factor-a production was enhanced in the tumor tissue. Furthermore, the enhanced antitumor effect was abolished by inactivating macrophages with carrageenan treatment. These results demonstrated that a bicistronic rAd harboring both suicide and chemokine genes in sequence exerted the enhanced, macrophage-dependent, antitumor effects in a model of HCC and support the use of this strategy for the treatment of HCC.

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Section: Enzme-linked Immunosorbent Assay (Elisa) For Mcp-1mentioning

confidence: 99%

Enhanced antitumor effects of a bicistronic adenovirus vector expressing both herpes simplex virus thymidine kinase and monocyte chemoattractant protein-1 against hepatocellular carcinoma

et al. 2003

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“…The concentration of MCP-1 was determined by enzyme-linked immunosorbent assay (ELISA) as described previously. 11 Briefly, each well of a 96-well microtiter plate was coated with monoclonal anti-human MCP-1 antibody (ME61; 1 mg ml À1 ) overnight at 41 1C. After washing, the plates were blocked by incubation with PBS containing 1% bovine serum albumin for 1 h at 37 1C.…”

Section: Elisa For Mcp-1mentioning

confidence: 99%

Membrane-bound form of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy in a model of hepatocellular carcinoma

et al. 2012

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“…The concentration of MCP-1 in this medium was determined by ELISA as described previously. 28 Briefly, each well of a 96-well microtiter plate (Nalgene, Rochester, NY) was coated with 0.05 M carbonate buffer (pH 9.6) containing monoclonal anti-human MCP-1 antibody (ME61; 1 mg/ml) overnight at 41C After washing with PBS containing 0.05% Tween-20 (PBS-T), the plates were blocked with PBS containing 1% BSA for 1 h at 371C and washed. Subsequently, diluted culture medium or varying concentrations of recombinant MCP-1 were added to duplicate wells and incubated for 2 h at 371C Following incubation, the plates were washed and incubated first with rabbit anti-MCP-1 antibodies (1 mg/ml), followed by alkaline phosphataseconjugated goat anti-rabbit antibody (1/12 000; Tago, Burlingame, CA), each for 2 h at 371C.…”

Section: Recombinant Adenoviral Vectorsmentioning

confidence: 99%

Monocyte chemoattractant protein-1 gene delivery enhances antitumor effects of herpes simplex virus thymidine kinase/ganciclovir system in a model of colon cancer

et al. 2005

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Suicide gene therapy using the herpes simplex virus thymidine kinase/ganciclovir (HSV-tk/GCV) system is a well-characterized tool for cancer gene therapy; however, it does not yet exhibit sufficient efficacy to cure patients of malignancies. We have reported that adenovirally delivered monocyte chemoattractant protein (MCP)-1 augmented the antitumor effects of the HSV-tk/GCV system in an athymic nude mouse model. The current study, which uses an immunocompetent mouse model of colon cancer, was designed to evaluate the antitumor effects of MCP-1 gene delivery in conjunction with this suicide gene therapy system. Subcutaneous tumor foci were directly transduced with both recombinant adenoviruses (rAds) expressing an HSV-tk gene and either of the MCP-1, CD80 and LacZ genes, followed by GCV administration. The growth of tumors was markedly suppressed by codelivery of HSV-tk and MCP-1 genes, which was exclusively associated with the recruitment of monocytes/macrophages, T helper 1 (Th1) cytokine gene expression and cytotoxic activity of the splenocytes. Furthermore, the antitumor effects were more efficient than that obtained by the combination of HSV-tk and CD80 genes. These results suggest an immunomodulatory effect of MCP-1 in the context of suicide gene therapy of colon cancer via orchestration of innate and acquired immune responses.

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A sensitive enzyme-linked immunosorbent assay for human interleukin-8

Cited by 108 publications

References 18 publications

Enhanced antitumor effects of a bicistronic adenovirus vector expressing both herpes simplex virus thymidine kinase and monocyte chemoattractant protein-1 against hepatocellular carcinoma

Enhanced antitumor effects of a bicistronic adenovirus vector expressing both herpes simplex virus thymidine kinase and monocyte chemoattractant protein-1 against hepatocellular carcinoma

Membrane-bound form of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy in a model of hepatocellular carcinoma

Monocyte chemoattractant protein-1 gene delivery enhances antitumor effects of herpes simplex virus thymidine kinase/ganciclovir system in a model of colon cancer

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