A sensitive high performance liquid chromatography–positive electrospray tandem mass spectrometry method for N7-[2-[(2-hydroxyethyl)thio]-ethyl]guanine determination

Yuxia, Wei; Yue, Lijun; Q, Liu; Chen, Jia; Xie, Jianwei

doi:10.1016/j.jchromb.2011.03.040

Cited by 15 publications

References 23 publications

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Methods for Retrospective Detection of Exposure to Toxic Scheduled Chemicals. Part B: Mass Spectrometric and Immunochemical Analysis of Covalent Adducts to Proteins and DNA

Noort¹,

Schans²,

Black³

2020

Encyclopedia of Analytical Chemistry

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In this article, an overview is presented of the methods that are currently available for retrospective detection of exposure to a number of chemical warfare agents (CWAs), based on adducts formed with macromolecules such as proteins. These methods can be applied for various purposes, e.g. diagnosis and dosimetry of exposure of casualties, confirmation of nonexposure, and verification of nonadherence to the Chemical Weapons Convention, health surveillance, and forensic purposes. The advantage of using protein adducts as biomarkers in comparison with free metabolites is that they are potentially much more long‐lived. The methods are predominantly based on liquid chromatography–mass spectrometry (LC–MS) analysis of enzymatic digests of the (modified) proteins or on selective removal of the specific adduct moiety from the protein, followed by gas chromatography–mass spectrometry (GC–MS) or LC–MS. Several of the methods have been successfully applied to actual cases and were shown to be highly retrospective.

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