A Sensitive High-Performance Liquid Chromatography Method for Analyzing Riboflavin in Fresh Fruits and Vegetables

Watada, Alley E.; Tran, Tony T.

doi:10.1080/01483918508074084

Cited by 28 publications

(14 citation statements)

References 6 publications

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“…Concentration of HPO 3 was set at 3% w/v as pH values are below 3. AA stability and its complete extraction are thus accomplished (Watada, 1982). About 1.25 mL of sample (pulp or juice) previously filtered is diluted with HPO 3 solution up to 1 L volume.…”

Section: Liquid Chromatographic Analysis Of Vitamin Cmentioning

confidence: 99%

Antioxidant compounds and antioxidant activity in acerola (Malpighia emarginata DC.) fruits and derivatives

Mezadri

Villaño

Fernández-Pachón

et al. 2008

Journal of Food Composition and Analysis

165

101

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Section: Liquid Chromatographic Analysis Of Vitamin Cmentioning

confidence: 99%

Antioxidant compounds and antioxidant activity in acerola (Malpighia emarginata DC.) fruits and derivatives

Mezadri

Villaño

Fernández-Pachón

et al. 2008

Journal of Food Composition and Analysis

165

101

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“…Ascorbic acid concentrations in HPLC eluates were calculated from absorption of standard quantities at 254 nm (Watada, 1982); absorption was linear over the range 0-30 µ . Luteal ascorbic acid co-localized with authentic ascorbic acid (Fisons Scientific Appar¬ atus, Loughborough, Leics, U.K.), and ascorbate levels measured in extracts of corpora lutea by HPLC were 117 + 4% (mean + s.e.m.)…”

Section: Pga Activitymentioning

confidence: 99%

Post-translational processing of oxytocin-neurophysin prohormone in the ovine corpus luteum: activity of peptidyl glycine α-amidating mono-oxygenase and concentrations of its cofactor, ascorbic acid

Sheldrick

Flint²

1989

Journal of Endocrinology

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Peptidyl glycine alpha-amidating mono-oxygenase (PGA), the terminal enzyme in the pathway of oxytocin synthesis, was measured in extracts of ovine corpora lutea throughout the oestrous cycle. Activity of PGA was low early in the cycle but increased between days 2 and 10 (from 2.3 to 9.0 pmol/mg protein per h) and remained high until day 15. Thereafter, activity declined rapidly at structural luteolysis and was low in corpora albicantia collected 18 and 20 days after ovulation (1.28 and 1.07 pmol/mg protein per h respectively). Luteal concentrations of ascorbic acid, a cofactor for PGA, were high (4.7 mumol/g wet wt tissue) by day 4 after oestrus; concentrations fell rapidly after day 15 (to 2.1 mumol/g on day 16). Concentrations of ascorbic acid were also high in the pituitary gland and in the adrenal medulla and cortex. Concentrations of oxytocin in luteal tissue, which were low (0.3 nmol/g wet wt) on day 2 after oestrus, were highest (2.73 nmol/g) on day 6 and declined thereafter (0.56 nmol/g on day 10, 0.08 nmol/g on day 15 and not detectable on days 18 and 20). Concentrations of oxytocin, progesterone, PGA and protein were measured in subcellular fractions obtained after density gradient centrifugation of extracts of corpora lutea collected on days 6, 7 and 12 of the oestrous cycle, and on day 7 from an anaesthetized ewe before and after treatment with the prostaglandin F2 alpha analogue, cloprostenol. PGA colocalized with particle-associated oxytocin in fractions of density 1.049-1.054 g/ml. Exogenous [3H]oxytocin and [3H]progesterone and endogenous progesterone localized in fractions of density 1.035 g/ml. Oxytocin and PGA were depleted from fractions of density 1.049-1.054 g/ml following cloprostenol treatment in vivo. Fractionation of extracts of ovine corpora lutea by high-performance liquid chromatography (HPLC) followed by radioimmunoassay and radioreceptor assay for oxytocin demonstrated the presence of at least two cross-reacting substances with elution characteristics distinct from oxytocin. Concentrations of these peptides increased as the cycle progressed. These compounds differed from the putative C-terminally extended post-translational processing intermediates, oxytocinyl-glycine, oxytocinyl-glycine-lysine and oxytocinyl-glycine-lysine-arginine, as indicated by their elution positions on HPLC and the specificities of the assays used to detect them, and no conclusions could be drawn on which post-translational processing step was rate-limiting in oxytocin synthesis. These data are consistent with the suggestion that post-translational processing of oxytocin-neurophysin prohormone takes place in secretory granules in luteal cells.(ABSTRACT TRUNCATED AT 400 WORDS)

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“…Total fibre content was determined according to Asp et al (1983). The ascorbic acid content in DAP was determined by HPLC (Watada 1982) using μBondapak C 18 (10 μm) column (30 cm X 4.6 mm id), Waters Co., Ireland with isocratic mobile phase consisting of 0.08 % ortho phosphoric acid. L-ascorbic acid was used as standard.…”

Section: Chemical Analysismentioning

confidence: 99%

Antioxidant and cyto/DNA protective properties of apple pomace enriched bakery products

et al. 2016

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Apple pomace (AP), the residue that remains after the extraction of juice from apple accounts for~25 % of total apple weight. Current study is aimed at identification of phytochemicals and utilization of Dehydrated apple pomace (DAP) in the preparation of bakery products with potential health benefits. DAP was prepared by drying the pomace obtained by crushing peeled apple fruits. DAP was incorporated into bakery products such as bun, muffin and cookies for value addition. Bioactivity such as free radical scavenging, cyto/DNA protectivity was evaluated in these products. DAP contained 17 g/100 g starch, 49.86 g/100 g fructose and 37 g/100 g dietary fibre. The phenolics and flavonoids content was 1.5 mg/g and 3.92 mg/g, respectively. Increase in DAP resulted in decreased volume and enhanced firmness of buns and muffins. DAP at 15 % in buns, 30 % in muffins and 20 % in cookies were found to be acceptable. DAP blended products exhibited better free radical scavenging as well as cyto/DNA protective properties suggesting the retention of bioactivity after baking. Addition of DAP potentially enhanced the bioactivity of the products evaluated.

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A Sensitive High-Performance Liquid Chromatography Method for Analyzing Riboflavin in Fresh Fruits and Vegetables

Cited by 28 publications

References 6 publications

Antioxidant compounds and antioxidant activity in acerola (Malpighia emarginata DC.) fruits and derivatives

Antioxidant compounds and antioxidant activity in acerola (Malpighia emarginata DC.) fruits and derivatives

Post-translational processing of oxytocin-neurophysin prohormone in the ovine corpus luteum: activity of peptidyl glycine α-amidating mono-oxygenase and concentrations of its cofactor, ascorbic acid

Antioxidant and cyto/DNA protective properties of apple pomace enriched bakery products

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