A sensitive impedance biosensor based on immunomagnetic separation and urease catalysis for rapid detection of Listeria monocytogenes using an immobilization-free interdigitated array microelectrode

Chen, Qi; Lin, Jianhan; Gan, Chengqi; Wang, Yuhe; Wang, Dan; Xiong, Yonghua; Lai, Weihua; Li, Yuntao; Wang, Maohua

doi:10.1016/j.bios.2015.06.007

Cited by 101 publications

(22 citation statements)

References 35 publications

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“…The gold nanoparticles (GNPs) with the size of ~20 nm were synthesized in advance according to our previously reported protocol 25 - 26 , and were modified with the aptamers against E. coli and the urease according to our previously reported protocol with slight modifications. Briefly, after the pH for 1 mL of GNPs was adjusted to 7.0 by K 2 CO 3 , 2 μL of streptavidin (1 mg/mL) was added drop-by-drop and incubated with gentle stirring for 1 h. Then, 12 μL of the biotinylated aptamers (10 μM) were added drop-by-drop and incubated with gentle stirring for 5 min to conjugate with the GNPs through streptavidin-biotin binding.…”

Section: Methodsmentioning

confidence: 99%

An Electrochemical Aptasensor Using Coaxial Capillary with Magnetic Nanoparticle, Urease Catalysis and PCB Electrode for Rapid and Sensitive Detection of Escherichia coli O157:H7

et al. 2017

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The continuous outbreaks of foodborne diseases have drawn public attentions to food safety. Early screening of foodborne pathogens is crucial to prevent and control of foodborne diseases. In this study, a novel electrochemical aptasensor was developed for rapid and sensitive detection of E. coli O157:H7 using the coaxial capillary with immune magnetic nanoparticles (MNPs) for specific separation of the target bacteria, the urease with urea for amplification of the impedance signals, and the PCB gold electrode for measurement of the impedance change. The streptavidin modified MNPs were conjugated with the biotinylated polyclonal antibodies (PAbs) to form the immune MNPs, and captured in the coaxial capillary with the line-up high gradient magnetic fields to separate the bacteria from the large volume of sample. Then, the gold nanoparticles (GNPs) were modified with the aptamers against E. coli and the urease, and injected into the capillary to react with the bacteria and form the MNP-PAb-bacteria-aptamer-GNP-urease complexes. Finally, the urease on the complexes was used to catalyze the hydrolysis of urea into ammonium ions and carbonate ions in the capillary, leading to the decrease in the impedance of the catalysate, which was measured by the gold plating PCB electrode. The impedance change of the catalysate and the concentration of the bacteria had a good linear relationship. This aptasensor was able to detect E. coli as low as 101 CFU/mL in 3 h, and the mean recovery of E. coli in the spiked pasteurized milk was ~99%. This proposed aptasensor has the potential for practical applications of foodborne pathogen detection due to its short detection time, high sensitivity and low cost.

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Section: Methodsmentioning

confidence: 99%

An Electrochemical Aptasensor Using Coaxial Capillary with Magnetic Nanoparticle, Urease Catalysis and PCB Electrode for Rapid and Sensitive Detection of Escherichia coli O157:H7

et al. 2017

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“…This biosensor platform detected the closely related bacterial strains, especially L. monocytogenes at very low concentrations (10 3 cfu/mL). Further, Chen et al (2015) combined immunomagnetic separation and urease catalysis to develop impedance biosensor for detection of L. monocytogenes using an antibody immobilization-free interdigitated array microelectrode. The technique was reported to detect as low as 300 cells of Listeria in both, the pure cultures and the spiked lettuce samples.…”

Section: Impedimetric Based Immuno Biosensorsmentioning

confidence: 99%

Biosensor for the detection of Listeria monocytogenes: emerging trends

Soni

Ahmad

Dubey

2018

Critical Reviews in Microbiology

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The early detection of Listeria monocytogenes (L. monocytogenes) and understanding the disease burden is of paramount interest. The failure to detect pathogenic bacteria in the food industry may have terrible consequences, and poses deleterious effects on human health. Therefore, integration of methods to detect and trace the route of pathogens along the entire food supply network might facilitate elucidation of the main contamination sources. Recent research interest has been oriented towards the development of rapid and affordable pathogen detection tools/techniques. An innovative and new approach like biosensors has been quite promising in revealing the foodborne pathogens. In spite of the existing knowledge, advanced research is still needed to substantiate the expeditious nature and sensitivity of biosensors for rapid and in situ analysis of foodborne pathogens. This review summarizes recent developments in optical, piezoelectric, cell-based, and electrochemical biosensors for Listeria sp. detection in clinical diagnostics, food analysis, and environmental monitoring, and also lists their drawbacks and advantages.

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“…The most frequent bacterial targets for biosensors are foodborne pathogens including Escherichia coli [13,14,15], Salmonella enterica [16,17,18], Listeria monocytogenes [19] and Vibrio parahaemolyticus [20]. The EIS biosensors for bacteria provide typical limits of detection (LODs) of 10 2 -10 4 CFU mL À 1 (colony-forming units) with no pre-enrichment and analysis time around 1-2 hours [19,21,22]. Further improvement of the sensitivity can be achieved by the use of suitable labels [23].…”

Section: Introductionmentioning

confidence: 99%

Amperometric Immunosensor for Rapid Detection of Honeybee Pathogen Melissococcus Plutonius

et al. 2019

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European foulbrood (EFB) is a honeybee larvae disease caused by a bacterium Melissococcus plutonius. An amperometric immunosensor based on a sandwich assay was developed for rapid point‐of‐care detection of this pathogen. An in‐house made anti‐Melissococcus antibody was immobilized to a gold surface of a screen‐printed sensor via self‐assembled monolayer of cysteamine activated with glutaraldehyde. The direct impedimetric detection of captured microbial cells was tested, however, a better performance was obtained after the formation of sandwich with the peroxidase‐labeled antibody in the amperometric mode. The label‐free assay was limited by higher non‐specific binding. The limit of detection of the immunosensor was 6.6×104 CFU mL−1 (colony‐forming units) with wide linear range between 105 CFU mL−1 and 109 CFU mL−1. The whole analysis was completed within 2 h, which is shorter compared to common laboratory diagnostic tools, such as enzyme‐linked immunosorbent assay or polymerase chain reaction. Furthermore, atomic force microscopy was used for confirmation of the bacteria presence on the electrode surface. The developed immunosensor was successfully employed in the analysis of real samples of honeybees and larvae. The achieved results demonstrate the potential of the amperometric immunosensor for practical in‐field diagnosis of EFB, which can prevent infection spreading and connected losses of honeybee colonies.

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A sensitive impedance biosensor based on immunomagnetic separation and urease catalysis for rapid detection of Listeria monocytogenes using an immobilization-free interdigitated array microelectrode

Cited by 101 publications

References 35 publications

An Electrochemical Aptasensor Using Coaxial Capillary with Magnetic Nanoparticle, Urease Catalysis and PCB Electrode for Rapid and Sensitive Detection of Escherichia coli O157:H7

An Electrochemical Aptasensor Using Coaxial Capillary with Magnetic Nanoparticle, Urease Catalysis and PCB Electrode for Rapid and Sensitive Detection of Escherichia coli O157:H7

Biosensor for the detection of Listeria monocytogenes: emerging trends

Amperometric Immunosensor for Rapid Detection of Honeybee Pathogen Melissococcus Plutonius

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