A Sensitive Isocratic Liquid Chromatography Assay for the Determination of Dipyridamole in Plasma with Electrochemical Detection

Barberi‐Heyob, Muriel; Merlin, Jean‐Louis; Pons, L.; Calco, M.; Weber, B.

doi:10.1080/10826079408013462

Cited by 14 publications

(3 citation statements)

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“…8 DIP like as other Extra Pharmacopeia drugs, is officially listed in Martindale. Numerous determination methods of the anti-oxidant DIP have been reported such as HPLC, [7][8][9][10][11][12][13] spectrophotometry, [14][15][16] fluorimetry, [17][18][19] phosphorimetry, 19,20 and chemiluminescence. 5,21 voltammetric and polarographic methods [22][23][24][25][26] were performed recently due to the linear relation between the peak current and the DIP concentration while electroanalytical techniques are similar to the redox biological reactions.…”

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confidence: 99%

Maghemite-Nanoparticles Enhanced Effects in Electrochemical Determination of Dipyridamole Utilizing Simultaneous Statistical Based Experimental Design Optimization

Afkhami

Moosavi

Madrakian

2013

J. Electrochem. Soc.

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Significant effects of maghemite (γ-Fe 2 O 3 ) nanoparticles on the voltammetric behavior of carbon paste electrode for bio determination of dipyridamole (DIP) were studied. The electrochemical performance of maghemite NPs modified carbon paste electrode (MMCPE) was investigated (compared to unmodified and the other manufactured modified electrodes) and the mechanism was studied by using differential pulse voltammetry (DPV) and cyclic voltammetry (CV) techniques. Herein we utilized statistical based experimental design for simple optimization of effective parameters on the response of the electrode. Results indicate that MMCPE can remarkably enhance electrocatalytic activity toward the oxidation of dipyridamole (DIP) in Britton−Robinson buffer at pH 2.0. Under optimal conditions a detection limit of 5.0 × 10 −9 mol L −1 (2.5 ng mL −1 ) was obtained by DPV. The advantageous method was then tested for the simple, rapid and sensitive electrochemical determination of DIP in pharmaceutical formulations and human plasma as real samples.One of the most influential transition metal oxides in technological importance are iron oxides, specially magnetite, Fe 3 O 4 , and maghemite, γ-Fe 2 O 3 . At the nanoscale these materials have great characterizations and potential applications. They use as catalytic materials, wastewater treatment adsorbents, pigments, flocculants, coatings, gas sensors, ion exchangers, lubrication, magnetic recording devices, magnetic data storage devices, toners and inks for xerography, magnetic resonance imaging, electrochemical biosensors, medicine and biomedical purposes. [1][2][3][4][5][6] Determination of drugs in pharmaceutical, clinical and biological environments is necessary due to the control of drug quality, stability, metabolism and the study of pharmacokinetics including their toxicity. 7 Dipyridamole (DIP) is commonly used as a coronary vasodilator and antiplatelet drug that binds to plasma protein over 99%. α-acid glycoprotein (AGP) and albumin are responsible for the high protein binding of DIP in plasma. It has been reported that DIP and its analogs enhance the in vitro activity of antimetabolite anticancer drugs by reducing AGP binding. 8 DIP like as other Extra Pharmacopeia drugs, is officially listed in Martindale. Numerous determination methods of the anti-oxidant DIP have been reported such as HPLC, 7-13 spectrophotometry, 14-16 fluorimetry, [17][18][19] phosphorimetry, 19,20 and chemiluminescence. 5,21 voltammetric and polarographic methods 22-26 were performed recently due to the linear relation between the peak current and the DIP concentration while electroanalytical techniques are similar to the redox biological reactions. They are also simple, time and cost effective, without clean-up procedure, portable, selective and sensitive. 27 For detection of the trace amounts of biologically important compounds the electrochemical methods using chemically modified electrodes have been widely used as sensitive and selective analytical techniques. Uses of carbon paste electrodes (CPE...

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Maghemite-Nanoparticles Enhanced Effects in Electrochemical Determination of Dipyridamole Utilizing Simultaneous Statistical Based Experimental Design Optimization

Afkhami

Moosavi

Madrakian

2013

J. Electrochem. Soc.

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“…Several analytical methods have been used for the determination of dipyridamole in different samples include chromatography [12,13,14,15,16,17,18], spectrophotometry [19,20,21], fluorimetry [22] phosphorimetry [23,24], fluorescence spectrometry [25,26], and voltammetry [27,28,29]. The polarographic method [27] achieved a detection limit sufficient only for tablets assay.…”

Section: Introductionmentioning

confidence: 99%

Assay of dipyridamole in human serum using cathodic adsorptive square-wave stripping voltammetry

Tawfik

Radi

2002

Anal Bioanal Chem

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A rapid and sensitive square-wave voltammetric procedure was optimized for the determination of dipyridamole after its adsorption preconcentration onto a hanging mercury drop electrode. The peak current of the first of the two peaks developed for this drug in Britton-Robinson buffer at pH 8.0 has been considered for the present analytical study. An accumulation potential of -1.0 V versus Ag/AgCl/KCl(s), pulse amplitude a =100 mV, scan increment Delta E =10 mV, and frequency f =120 Hz were the optimal experimental parameters. Dipyridamole can be determined in the concentration range of 9.0 x 10(-9) to 5.0 x 10(-6) M using accumulation times of 30-300 s. A detection limit of 4.0 x 10(-11) M was achieved after a 300 s accumulation time. Applicability to serum samples was illustrated. The average recoveries for dipyridamole spiked to serum at 0.25-4.50 micro g ml(-1) were 96.0-102.0%, and the higher standard deviation was 2.9%. A detection limit of 0.06 micro g mL(-1) of serum was obtained.

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“…Dipyridamole Injection is a parenteral solution consisting of dipyridamole (active), tartaric acid and polyethylene glycol 600 in sterile water for injection. The analysis of dipyridamole has been determined by spectrophotometry [3], adsorptive stripping voltammetry [4], and HPLC with UV [5][6][7] and electrochemical detection [8,9]. None of these methods provide selectivity data for potential degradation products in the finished product, although two methods [5,6] do demonstrate selectivity with respect to the raw material.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a stability-indicating HPLC method for the determination of degradation products in dipyridamole injection

Zhang¹,

Miller²

1997

Chromatographia

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SummaryThe development and subsequent validation of an isocratic high-performance liquid chromatographic (HPLC) procedure employing ultraviolet (UV) detection for the determination of degradation products in Dipyridamole Injection is reported. The development of this assay involved the evaluation of several factors including buffer type, ionic strength, pH, organic composition, and column type. The described method is simple, reproducible, accurate, and selective. The precision, relative standard deviation (RSD), amongst five sample preparations for total degradation products was not more than (NMT) 10.2 %, while the individual degradation products were NMT 12.1 %. Intermediate precision, as determined from fifteen sample preparations, generated by two Analysts on different HPLC systems over three days, exhibited an RSD for total and individual degradation products of 8.2 % and NMT 27.5 %, respectively. The mean absolute recovery of dipyridamole using the described method is 102.1 + 1. 9 %, (mean + SD, n = 12) over the concentration range of 0.03 % to 5.0 % of its label claim of 5 mg mL -1. The limit of detection and limit of quantitation were 0.1 and 0.3 ~tg mL -1, respectively. The linearity of the peak response was verified with respect to dipyridamole concentration over a range of 0.3 and 50 ~tg mL -1 (0.03 % to 5.0 % label claim). The Standard and Assay Preparations are stable for up to 48 hours at room temperature. The selectivity was evaluated by subjecting the finished product (Dipyridamole Injection) to thermal, acidic, basic, oxidative and fluorescent radiation stress conditions. No interference in the analysis of degradation products was observed, showing the method is stabilityindicating.

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A Sensitive Isocratic Liquid Chromatography Assay for the Determination of Dipyridamole in Plasma with Electrochemical Detection

Cited by 14 publications

References 4 publications

Maghemite-Nanoparticles Enhanced Effects in Electrochemical Determination of Dipyridamole Utilizing Simultaneous Statistical Based Experimental Design Optimization

Maghemite-Nanoparticles Enhanced Effects in Electrochemical Determination of Dipyridamole Utilizing Simultaneous Statistical Based Experimental Design Optimization

Assay of dipyridamole in human serum using cathodic adsorptive square-wave stripping voltammetry

Development and validation of a stability-indicating HPLC method for the determination of degradation products in dipyridamole injection

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