A sensitive, label free electrochemical aptasensor for ATP detection

Li, Wang; Nie, Zhou; Xu, Xiahong; Shen, Qinpeng; Deng, Chao; Chen, Jinhua; Yao, Shouzhuo

doi:10.1016/j.talanta.2009.01.009

Cited by 79 publications

(42 citation statements)

References 28 publications

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“…The sensor could be used to detect adenosine from real human serum samples. The same strategy for ATP detection was reported by Yao's group with LOD of 1.0×10 −7 M for ATP [120]. Wang et al reported an electrochemical detection approach for PDGFand AuNP-mediated amplification technique by using electrochemical probe [Ru(NH 3 ) 5 Cl] 2+ [121].…”

Section: Aunps As Probe Vectors In Electrochemical Aptasensorsmentioning

confidence: 99%

“…Besides the probe MB, the electroactive complex, [Ru (NH 3 ) 6 ] 3+ (RuHex), which could bind to anionic phosphates of DNA strands completely through electrostatic interactions, was widely used as a signaling transducer in electrochemical aptasensors [51][52][53][54][55][56][119][120][121][122]. Zhang et al reported a sensing strategy based on switching structures of aptamers using RuHex as an electrochemical probe [119].…”

Section: Aunps As Probe Vectors In Electrochemical Aptasensorsmentioning

confidence: 99%

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Analytical potential of gold nanoparticles in functional aptamer-based biosensors

Wang

2010

Bioanal Rev

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Gold nanoparticles (AuNPs) exhibit many predominant capabilities such as high biocompatibility, chemical stability, strong localized surface plasmon resonance absorption, and high extinction coefficient in the visible region. These properties have enabled the extensive use of AuNPs in optical and electrochemical biosensors. As a kind of functional nucleic acids, aptamers can be considered as a valid alternative to antibodies or other bio-receptors and have been widely employed to develop novel biosensors. We are summarizing here the state of the art of AuNP-based biosensors that use functional aptamers as molecular recognition elements. In many cases, AuNPs themselves can be used as a probe for detection, such as various colorimetric aptasensors and fluorescent aptasensors. They also can be used as probe vectors to enlarge detection signals and to increase the number of conceivable substrates in electrochemical aptasensors.

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Section: Aunps As Probe Vectors In Electrochemical Aptasensorsmentioning

confidence: 99%

Section: Aunps As Probe Vectors In Electrochemical Aptasensorsmentioning

confidence: 99%

Analytical potential of gold nanoparticles in functional aptamer-based biosensors

Wang

2010

Bioanal Rev

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“…In addition to continual advancements in sensing capabilities for electrochemistry-based detection, many sensing designs have been applied in complex sample matrixes including serum [58], urine [59], and cell extracts [60]. Furthermore, the structure-switching design has also been used for multiplex detection.…”

Section: Structure-switching Dna Aptamers In Electrochemical Sensorsmentioning

confidence: 99%

Exploration of Structure-Switching in the Design of Aptamer Biosensors

Lau

2013

Advances in Biochemical Engineering/Biotechnology

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The process of "structure-switching" enables biomolecular switches to function as effective biosensing tools. Biomolecular switches can be activated or inactivated by binding to a specific target that triggers a precise conformational change in the biomolecules involved. Although many examples of aptamer-based biomolecular switches can be found in nature, substantial effort has been made in the last decade to engineer structure-switching aptamer sensors by coupling aptamers to a signal transduction method to generate a readout signal upon target binding to the aptamer domain. This chapter focuses on the progress of research on engineered structure-switching aptamer sensors. We begin by discussing the origin of the structure-switching aptamer design, highlight the key developments of structure-switching DNA aptamers for fluorescence-, electrochemistry-, and colorimetry-based detection, and introduce our recent efforts in exploring RNA aptamers to create structure-switching molecular sensors.

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“…Aptamer-functionalized AuNPs have been previously attached to gold surfaces [47,48], however this is the first systematic study using a hydrogel surface.…”

Section: Adenosine-induced Aunp Releasementioning

confidence: 99%

Stimuli-responsive releasing of gold nanoparticles and liposomes from aptamer-functionalized hydrogels

El-Hamed¹,

Dave²,

Liu³

2011

Nanotechnology

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Controlled release of therapeutic agents is important for improving drug efficacy and reducing toxicity.Recently, hydrogels have been used for controlled release applications. While majority of the previous work focused on releasing the cargo in response to physical stimuli such as temperature, light, electric field, and pH, we aim to trigger cargo release in the presence of small metabolites. In our system a DNA aptamer that can bind to adenosine, AMP and ATP was used as a linker to attach either DNAfunctionalized gold nanoparticles and or liposomes to DNA-functionalized hydrogels. In the presence of the metabolite, both the nanoparticle and liposome cargos were released. The effect of salt, temperature, target concentration, and drying has been systematically studied. Interestingly, we found that the gel can be completely dried while retaining the DNA linkages, where upon rehydration; the adenosine-induced releasing was still achieved. Our work demonstrates that aptamers can be used to control release of drugs and other materials attached to hydrogels.

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A sensitive, label free electrochemical aptasensor for ATP detection

Cited by 79 publications

References 28 publications

Analytical potential of gold nanoparticles in functional aptamer-based biosensors

Analytical potential of gold nanoparticles in functional aptamer-based biosensors

Exploration of Structure-Switching in the Design of Aptamer Biosensors

Stimuli-responsive releasing of gold nanoparticles and liposomes from aptamer-functionalized hydrogels

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