A sensitive lanthanide label array method for rapid fingerprint analysis of plant polyphenols based on time-resolved luminescence

Abstract

For the first time, a novel lanthanide label array method was developed for rapid fingerprint analysis of plant polyphenols in a high throughput format.

Isolation, detection, and quantification of hydrolyzable tannins of the biosynthetic pathway by liquid chromatography coupled with tandem mass spectrometry

“…The HTs were identified by comparing the m/z values of the precursors and typical product ions, exact retention times and UV spectra with the published literature. Most of the plant species used in the current investigations have been investigated and detailed MS spectra have been published in our recent paper with the identification of major monomeric and oligomeric HTs 11 . All the major HTs have been extensively characterized previously by researchers from our group and others 15,17–24 .…”

“…A reversed‐phase Acquity UPLC BEH Phenyl column (100 mm × 2.1 mm i.d., 1.7 μm; Waters Corp., Wexford, Ireland) was employed. The chromatographic and tandem mass spectrometric (MS/MS) operating conditions were the same as reported in our previously published UHPLC‐DAD/ESI‐QqQ MS/MS method 11 . Samples were diluted with water as needed to keep them within the detection limit and filtered using VWR® 0.2 μm PTFE syringe filters before the analysis.…”

“…GTs contain six or more galloyl units whereas simple gallic acid derivatives contain five galloyl units or fewer. ETs are more diverse and around 600 of them have been identified and characterized – from simple monomers to complex oligomers 10‐12,15,17‐24 . Typically, two major units, namely, hexahydroxydiphenic acid (HHDP) and nonahydroxyterphenoyl (NHTP), either one of which or both can attach to the sugar moiety to build the basic structure of HTs.…”