A sensitive liquid chromatographic method for the analysis of isovaleric and valeric acids in urine as fluorescent derivatives

trans, trans-Muconic acid (MA) is a polar metabolite of benzene and used as a biomarker for monitoring human exposure to benzene. Because MA is a trace metabolite, sensitive method is required for its detection. In addition, MA is a highly polar compound with dicarboxyl functions that could incur unfavorable adsorption on silica-based stationary phase usually used for separation. To address these problems, we planned to derivatize MA with a fluorescent reagent 2-(2-naphthoxy)ethyl-2-(piperidino)ethanesulfonate to give a naphthoxy derivative of MA for improving detection sensitivity and chromatographic properties. Surprisingly, the resulting derivative shows no fluorescent activity (lambda(ex): 226 nm; lambda(em): 350 nm). The negative results could be used as an instructive example for discussing on fluorescence quenching.

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Characterization of odorants in human urine using a combined chemo-analytical and human-sensory approach: a potential diagnostic strategy

Wagenstaller

Buettner

2012

Metabolomics

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A sensitive liquid chromatographic method for the analysis of isovaleric and valeric acids in urine as fluorescent derivatives

Cited by 5 publications

References 16 publications

Analysis and Chemistry of Human Odors

Analysis and Chemistry of Human Odors

A Non-fluorescent Derivative from Derivatizing trans, trans-Muconic Acid with 2-(2-Naphthoxy)ethyl-2-(piperidino)ethanesulfonate

Characterization of odorants in human urine using a combined chemo-analytical and human-sensory approach: a potential diagnostic strategy

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