A Sensitive Method for Estimating Carboxyhemoglobin and Its Applications

Ishikawa, Kiyofumi; Fujibayashi, Shimu; Kuno, Yukikazu; Omichi, M

doi:10.1539/joh1959.14.123

Cited by 1 publication

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“…After standing for 20 min, the samples were centrifuged at 6000 rpm for 20 min and measured at various absorbances, using Nano Drop One/One (Thermo Fisher, Waltham, MA, USA). A range of 538 nm [ 43 , 44 , 45 ] was used for carboxyhemoglobin, 383 nm [ 46 ] was used for total heme pigment, and 455–575 nm [ 47 , 48 ] was used for bilirubin.…”

Section: Methodsmentioning

confidence: 99%

Study on the Mechanism of the Pink Tooth Phenomenon Using Bovine Teeth: A Pilot Study

Sumi,

Minegishi,

Ohta

et al. 2023

Diagnostics

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The pink teeth phenomenon has occasionally been observed in forensic autopsies. This study aimed to establish an experimental pink tooth model and an objective color tone evaluation method in order to clarify changes in the color tone of teeth and the relationship with hemoglobin monoxide and its decomposition products and with red pigment-producing bacteria, under various external environmental factors. It was confirmed that the color tone evaluation with ΔE and the L*C*h color space was useful. The results of various examinations using this model showed that color development was suppressed under aerobic conditions, faded early under light, became bright red under a low temperature and showed a tendency to be reddish at 3 days under high humidity and in the presence of soft tissue. The biochemical analysis revealed a significant increase in carboxyhemoglobin at 7 days and a tendency toward increasing the total heme pigment and bilirubin levels over time. The bacteriological analysis revealed that red pigment-producing bacteria increased over time but that the color faded after 7 days. These results suggest that putrefaction greatly affects the pink teeth phenomenon, whereas red pigment-producing bacteria have little effect on the occurrence of pink teeth. However, further studies are needed to clarify bacteriological involvement.

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