A Sensitive Method for the Determination of Urinary Mercapturic Acids for use in Biological Monitoring

Kress, Gabriele; Pentz, R.

doi:10.1007/978-3-642-74117-3_11

Cited by 2 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protein content was measured by the method of Lowry et al [30]. Urinary mercapturic acid excretion-The urinary mercapturic acid level was analyzed by HPLC after the extraction by the method of Kress and Pentz [31]. Fatty acid composition-Fatty acid methyl esters of dietary lipids were prepared and analyzed by GLC according to a previous study [10].…”

Section: Malondialdehyde (Mda) + 4-hydroxy-2-alkenals (4-hae)-mentioning

confidence: 99%

Docosahexaenoic Acid-Induced Lipid Peroxidation and Urinary Excretion of Mercapturic Acid in Rats

Sekine¹,

Kubo²,

Tadokoro³

et al. 2006

J. Clin. Biochem. Nutr.

View full text Add to dashboard Cite

Summary We hypothesized a suppressive mechanism for dietary docosahexaenoic acid (DHA)-induced tissue lipid peroxidation in which the degradation products, especially aldehydic compounds, are conjugated with glutathione (GSH) through catalysis by glutathione S-transferases (GSTs), and then excreted into urine as mercapturic acids. Sprague-Dawley rats were fed a diet containing DHA (8.4 % of total energy) for 31 days. Lipid peroxides in the liver and kidney, liver GST and urinary excretion of mercapturic acid were measured. The lipid peroxide levels in the liver and kidney except the liver aldehydic compounds were higher, and the urinary excretion of mercapturic acid also tended to be higher in the DHA-fed rats although the activity of GST was not increased after DHA intake. We presume from our results that a proportion of the lipid peroxidation-derived aldehydic degradation products might be excreted into urine as mercapturic acid after intake of DHA, thus suppressing the accumulation of aldehydic products in tissues, particularly in the liver.

show abstract

Section: Malondialdehyde (Mda) + 4-hydroxy-2-alkenals (4-hae)-mentioning

confidence: 99%

Docosahexaenoic Acid-Induced Lipid Peroxidation and Urinary Excretion of Mercapturic Acid in Rats

Sekine¹,

Kubo²,

Tadokoro³

et al. 2006

J. Clin. Biochem. Nutr.

View full text Add to dashboard Cite

show abstract

“…To determine a temporal change in the urinary excretion of mercapturic acids, whole urine was collected on days 0, 7, 14, 21, and 28, respectively. The urinary mercapturic acid was analyzed by HPLC after the extraction by the method of Kress and Pentz [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of Docosahexaenoic Acid Ingestion on Temporal Change in Urinary Excretion of Mercapturic Acid in ODS Rats

Sekine

Kubo

Tadokoro

et al. 2007

J. Clin. Biochem. Nutr.

View full text Add to dashboard Cite

Summary We hypothesized a suppressive mechanism for docosahexaenoic acid (22:6n-3; DHA)-induced tissue lipid peroxidation in which the degradation products, especially aldehydic compounds, are conjugated with glutathione through catalysis by glutathione S-transferases, and then excreted into urine as mercapturic acids. In the present study, ascorbic acid-requiring ODS rats were fed a diet containing DHA (3.6% of total energy) for 31 days. Lipid peroxides including degradation products and their scavengers in the liver and kidney were determined, and the temporal change in the urinary excretion of mercapturic acids was also measured. The activity of aldehyde dehydrogenase, which catalyzes the oxidation and detoxification of aldehydes, tended to be higher in the liver of DHA-fed rats. The levels of lipid peroxides as measured by thiobarbituric acid-reactive substances and aldehydic compounds were higher and that of α-tocopherol was lower in the liver, and the pattern of temporal changes in the urinary excretion of mercapturic acids was also different between the n-6 linoleic acid and DHA-fed rats. Accordingly, we presume from these results that after dietary DHAinduced lipid peroxidation, a proportion of the lipid peroxidation-derived aldehydic degradation products is excreted into urine as mercapturic acids.

show abstract

A Sensitive Method for the Determination of Urinary Mercapturic Acids for use in Biological Monitoring

Cited by 2 publications

References 3 publications

Docosahexaenoic Acid-Induced Lipid Peroxidation and Urinary Excretion of Mercapturic Acid in Rats

Docosahexaenoic Acid-Induced Lipid Peroxidation and Urinary Excretion of Mercapturic Acid in Rats

Effect of Docosahexaenoic Acid Ingestion on Temporal Change in Urinary Excretion of Mercapturic Acid in ODS Rats

Contact Info

Product

Resources

About