A Sensitive, Portable Microfluidic Device for SARS-CoV-2 Detection from Self-Collected Saliva

Yang, Jianing; Kidd, Mark; Nordquist, Alan; Smith, S. D.; Hurth, Cédric; Modlin, Irvin M.; Zenhausern, Frédéric

doi:10.3390/idr13040097

Cited by 15 publications

(14 citation statements)

References 41 publications

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“…In addition to the literature, many companies have developed point-of-care (POC) testing systems for SARS-CoV-2 detection (Yang J. et al, 2021). For example, the Cepheid Xpert Xpress SARS-CoV-2/Flu/RSV system (CA, USA) can provide rapid detection of SARS-CoV-2 in 25 min and provide results for four pathogens in 36 min, with less than 1 min hands-on time.…”

Section: Biosensors and Microfluidicsmentioning

confidence: 99%

Nucleic acid testing of SARS-CoV-2: A review of current methods, challenges, and prospects

Zhu

Zhang

et al. 2022

Front. Microbiol.

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Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has brought a huge threat to public health and the global economy. Rapid identification and isolation of SARS-CoV-2-infected individuals are regarded as one of the most effective measures to control the pandemic. Because of its high sensitivity and specificity, nucleic acid testing has become the major method of SARS-CoV-2 detection. A deep understanding of different diagnosis methods for COVID-19 could help researchers make an optimal choice in detecting COVID-19 at different symptom stages. In this review, we summarize and evaluate the latest developments in current nucleic acid detection methods for SARS-CoV-2. In particular, we discuss biosensors and CRISPR-based diagnostic systems and their characteristics and challenges. Furthermore, the emerging COVID-19 variants and their impact on SARS-CoV-2 diagnosis are systematically introduced and discussed. Considering the disease dynamics, we also recommend optional diagnostic tests for different symptom stages. From sample preparation to results readout, we conclude by pointing out the pain points and future directions of COVID-19 detection.

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Section: Biosensors and Microfluidicsmentioning

confidence: 99%

Nucleic acid testing of SARS-CoV-2: A review of current methods, challenges, and prospects

Zhu

Zhang

et al. 2022

Front. Microbiol.

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“…Fluorescence is the most extensively used approach due to its high sensitivity and abundance of fluorophores and labeling chemistries [ 80 ]. A quantitative nanofluidic assay based on qPCR developed by Fassy et al, three-step microfluidic nano-scale qPCR based on a microfluidic chip designed by Xie et al, microfluidic chip PCR technology developed by Francesca Dragoni et al, microfluidic disc-direct RT-qPCR assay developed by Ji M et al, portable MiDAS for SARS-CoV-2 nucleic acids detection developed by Yang, J et al, detect SARS-CoV-2 nucleic acids by the fluorescence-based detector ( Table 3 ) [ 81 , 82 , 83 , 84 , 85 ].…”

Section: Microfluidics In Sars-cov-2mentioning

confidence: 99%

“…On the other hand, the fluorescence signal is collected as images from a CMOS camera using microfluidic chip-based PCR technology [ 83 , 84 ]. A portable microfluidic-based integrated detection analysis system (MiDAS) developed by Yang, J et al detects SARS-CoV-2 nucleic acids in saliva samples in less than two hours with a 1000 copies/mL limit of detection [ 85 ]. For COVID-19 molecular testing, NP swabs are considered a standard sample source.…”

Section: Microfluidics In Sars-cov-2mentioning

confidence: 99%

“…However, other studies present saliva as a good alternative biofluid sample [ 91 , 92 , 93 ]. The MiDAS SARS-CoV-2 system, employing detection of antigens from saliva, can be deployed in areas/countries with limited resources due to the low cost, lack of cross-contamination, automation, as well as high sensitivity followed mobility [ 85 ]. The detected biomolecules are cDNA for all of the stated assays ( Table 3 ).…”

Section: Microfluidics In Sars-cov-2mentioning

confidence: 99%

“…As for the material, PMMA was frequently used, followed by PDMS, polycarbonate, 192.24 IFC, nylon, glass, clear resin, and clear methacrylate-based resin [ 81 , 84 , 85 , 94 , 102 , 104 , 108 , 109 , 110 ]. Among fluid manipulation techniques, centrifugal force was the most commonly used strategy, followed by electrochemical pumping, capillary action, manual pipetting, hydrostatic pressure, isotachophoresis, and syringe pump [ 82 , 84 , 85 , 94 , 102 , 104 , 105 , 109 ]. Yang et al used manual pipetting followed by capillary action for the fluid manipulation strategy [ 107 ].…”

Section: Materials and Fluid Manipulation Techniquementioning

confidence: 99%

See 2 more Smart Citations

Microfluidics Technology in SARS-CoV-2 Diagnosis and Beyond: A Systematic Review

Jamiruddin

Meghla

Islam

et al. 2022

Life

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With the progression of the COVID-19 pandemic, new technologies are being implemented for more rapid, scalable, and sensitive diagnostics. The implementation of microfluidic techniques and their amalgamation with different detection techniques has led to innovative diagnostics kits to detect SARS-CoV-2 antibodies, antigens, and nucleic acids. In this review, we explore the different microfluidic-based diagnostics kits and how their amalgamation with the various detection techniques has spearheaded their availability throughout the world. Three other online databases, PubMed, ScienceDirect, and Google Scholar, were referred for articles. One thousand one hundred sixty-four articles were determined with the search algorithm of microfluidics followed by diagnostics and SARS-CoV-2. We found that most of the materials used to produce microfluidics devices were the polymer materials such as PDMS, PMMA, and others. Centrifugal force is the most commonly used fluid manipulation technique, followed by electrochemical pumping, capillary action, and isotachophoresis. The implementation of the detection technique varied. In the case of antibody detection, spectrometer-based detection was most common, followed by fluorescence-based as well as colorimetry-based. In contrast, antigen detection implemented electrochemical-based detection followed by fluorescence-based detection, and spectrometer-based detection were most common. Finally, nucleic acid detection exclusively implements fluorescence-based detection with a few colorimetry-based detections. It has been further observed that the sensitivity and specificity of most devices varied with implementing the detection-based technique alongside the fluid manipulation technique. Most microfluidics devices are simple and incorporate the detection-based system within the device. This simplifies the deployment of such devices in a wide range of environments. They can play a significant role in increasing the rate of infection detection and facilitating better health services.

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Emerging Trends and Future Prospects in Microfluidic Systems for Prevention and Diagnosis of Infection Pathogens

Dabbagh Moghaddam,

Rafiee,

Setayeshfar

et al. 2024

Adv Materials Technologies

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Infectious diseases caused by bacteria, viruses, fungi, and parasites pose a significant societal challenge. In response, scientists are developing advanced technology to enhance the prevention, diagnosis, and treatment of such diseases. One such promising technology is microfluidic systems, which are utilized in organ‐on‐a‐chip systems to replicate the microenvironments of organs. These systems have potential applications in drug screening, disease modeling, and personalized medicine. This review provides an overview of recent advances in organ‐on‐a‐chip platforms and their potential for preventing and diagnosing various infections. After discussing traditional techniques employed in studying infectious diseases, the role of microfluidic platforms in detecting infections is delved in. It is expound on advanced microfluidic‐based strategies for infection diagnosis, such as the polymerase chain reaction‐based microfluidic devices, enzyme linked immunosorbent assay‐based microfluidic devices, hierarchical nanofluidic molecular enrichment systemand µWestern blotting‐based microfluidic devices, and smartphone‐based microfluidic devices. Additionally, future research challenges and perspectives are discussed on microfluidic systems in biomedical and regenerative medicine applications. Consequently, microfluidic platforms have the potential to serve as fundamental frameworks for understanding infectious diseases, thereby leading to personalized regenerative medicine. hierarchical nanofluidic molecular enrichment system

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A Sensitive, Portable Microfluidic Device for SARS-CoV-2 Detection from Self-Collected Saliva

Cited by 15 publications

References 41 publications

Nucleic acid testing of SARS-CoV-2: A review of current methods, challenges, and prospects

Nucleic acid testing of SARS-CoV-2: A review of current methods, challenges, and prospects

Microfluidics Technology in SARS-CoV-2 Diagnosis and Beyond: A Systematic Review

Emerging Trends and Future Prospects in Microfluidic Systems for Prevention and Diagnosis of Infection Pathogens

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