A sensitive signal-on electrochemical assay for MTase activity using AuNPs amplification

He, Xiaoxiao; Su, Jing; Wang, Yonghong; Wang, Kemin; Ni, Xiaoqi; Chen, Zhifeng

doi:10.1016/j.bios.2011.07.035

Cited by 84 publications

(40 citation statements)

References 30 publications

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“…18 Furthermore, in order to simplify the process, shorten the analysis time, improve the sensitivity, and lower the cost, many new techniques and methods such as fluorescence methods based on hairpin DNA probe, [19][20][21] enzyme immunoassay based on gold nanoparticles, 22,23 surface-enhanced Raman spectroscopy, 24,25 chemiluminescence assay, 26,27 electrochemical methods, [28][29][30][31] and so on have been designed to evaluate the activity of DNA MTase.…”

Section: Yu Et Almentioning

confidence: 99%

Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample

Yu¹,

Xiong²,

Yu³

et al. 2018

IJN

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Background DNA methyltransferase 1 (DNMT1), a dominant enzyme responsible for the transfer of a methyl group from the universal methyl donor to the 5-position of cytosine residues in DNA, is essential for mammalian development and closely related to cancer and a variety of age-related chronic diseases. DNMT1 has become a useful biomarker in early disease diagnosis and a potential therapeutic target in cancer therapy and drug development. However, till now, most of the studies on DNA methyltransferase (MTase) detection have focused on the prokaryote MTase and its activity. Methods A magnetic fluorescence-linked immunosorbent assay (FLISA) using CdSe/ZnS quantum dots as fluorescent probes was proposed for the rapid and sensitive detection of the DNMT1 level in this study. Key factors that affect the precision and accuracy of the determination of DNMT1 were optimized. Results Under the optimal conditions, the limit of detection was 0.1 ng/mL, the linear range was 0.1–1,500 ng/mL, the recovery was 91.67%–106.50%, and the relative standard deviations of intra- and inter-assays were respectively 5.45%–11.29% and 7.03%–11.25%. The cross-reactivity rates with DNA methyltransferases 3a and 3b were only 4.0% and 9.4%, respectively. Furthermore, FLISA was successfully used to detect the levels of DNMT1 in human serum samples, and compared with commercial enzyme-linked immunosorbent assay (ELISA) kits. The results revealed that there was a good correlation between FLISA and commercial ELISA kits (correlation coefficient r =0.866, p =0.001). The linear scope of FLISA was broader than ELISA, and the measurement time was much shorter than ELISA kits. Conclusion These indicated that the proposed FLISA method was sensitive and high throughput and can quickly screen the level of DNMT1 in serum samples.

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Section: Yu Et Almentioning

confidence: 99%

Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample

Yu¹,

Xiong²,

Yu³

et al. 2018

IJN

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“…Zhao et al [101] fabricated an ultrasensitive and highly specific EC aptasensor for thrombin based on amplification of aptamer-AuNPs-horseradish peroxidase conjugates. He et al [102] reported a label-free and sensitive signal-on electrochemical assay for MTase quantification and activity analysis using AuNPsamplificatio. In 2016, an ultrasensitive DNA biosensor was developed through in situ labeling of electroactive melamine-Cu 2+ complex (Mel-Cu 2+ ) on the end of hairpin-like probe using AuNPs as the signal amplification platform by our group [103].…”

Section: Biomoleculesmentioning

confidence: 99%

Advanced AuNMs as nanomedicine’s central goals capable of active targeting in both imaging and therapy in biomolecules

Lh¹

2017

Glob Drugs Therap

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“…However, in addition to relatively high detection limit, the sensing platforms also suffer from time-consuming DNA conjugation on AuNPs or polymerization process. Very recently, the electrochemical and electrogenerated chemiluminescence (ECL) biosensors have been described for DNA MTase activity assay with improved detection limits even down to 0.02 U/mL level (He et al, 2011;Li et al, 2012;Liu et al, 2011;Su et al, 2012;Wu et al, 2012). In addition, a novel bioluminescence assay based on protein expression in vitro has been reported for monitoring DNA MTase activity .…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive fluorescence assay of DNA methyltransferase activity via methylation-sensitive cleavage coupled with nicking enzyme-assisted signalamplification

Zhao

Chen

et al. 2013

Biosensors and Bioelectronics

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A sensitive signal-on electrochemical assay for MTase activity using AuNPs amplification

Cited by 84 publications

References 30 publications

Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample

Magnetic immunoassay using CdSe/ZnS quantum dots as fluorescent probes to detect the level of DNA methyltransferase 1 in human serum sample

Advanced AuNMs as nanomedicine’s central goals capable of active targeting in both imaging and therapy in biomolecules

Highly sensitive fluorescence assay of DNA methyltransferase activity via methylation-sensitive cleavage coupled with nicking enzyme-assisted signalamplification

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