A sensor for glycoproteins based on dendritic gold nanoparticles electrodeposited on a gold electrode and modified with a phenylboronic acid

Shen, Dongdong; Liu, Yunchun; Fang, Ying; Yang, Zhousheng

doi:10.1007/s10008-014-2636-3

Cited by 9 publications

(4 citation statements)

References 35 publications

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“…The assays of glycoproteins were performed using the smooth gold-coated silicon wafers in the neutral PB solutions (pH 7.4). In comparison with the assays of glycoproteins using nanoparticle powders in alkaline solutions (preferential formation of boronate bonds), the measured detection limit (10 ng/mL) in our case was very low, which is able to directly determine glycoproteins without the need of enrichment using the SERS sandwich assay. The gold-coated silicon wafers used were easy to separate, detect, and reuse.…”

Section: Resultsmentioning

confidence: 99%

Sensitive Glycoprotein Sandwich Assays by the Synergistic Effect of In Situ Generation of Raman Probes and Plasmonic Coupling of Ag Core–Au Satellite Nanostructures

Chen

et al. 2016

ACS Appl. Mater. Interfaces

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Sensitive surface-enhanced Raman scattering (SERS) assays of glycoproteins have been proposed using p-aminothiophenol (PATP)-embedded Ag core-Au satellite nanostructures modified with p-mercaptophenylboronic acid (PMBA) and the self-assembled monolayer of PMBA on a smooth gold-coated wafer. The apparent Raman probe PATP on the surfaces of the Ag cores underwent a photodimerization to generate 4,4'-dimercaptoazobenzene (DMAB) in situ upon excitation of laser, and the in situ generated DMAB acted as the actual Raman probe with considerably strong SERS signals, which was further enhanced by the plasmonic coupling of the Ag core-Au satellite nanostructures due to the synergistic effect. The sandwich assays of glycoproteins showed high sensitivity and excellent selectivity against nonglycoproteins. The Ag core-Au satellite SERS nanostructures can be used for highly sensitive SERS assays of other analytes.

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Section: Resultsmentioning

confidence: 99%

Sensitive Glycoprotein Sandwich Assays by the Synergistic Effect of In Situ Generation of Raman Probes and Plasmonic Coupling of Ag Core–Au Satellite Nanostructures

Chen

et al. 2016

ACS Appl. Mater. Interfaces

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“…Electrodeposited gold microstructures with nanoscale dendritic morphology were used as a sensor for a glycoprotein HRP based on the carbohydrate-boronic acid interaction [74]. Electrodeposition from 0.1 M of Na 2 SO 4 and 30 mM of HAuCl 4 at −0.6 V (versus Ag/AgCl) for 400 s onto a gold electrode produced dendritic forms that were microns in size, with dendritic morphology on the nanoscale.…”

Section: Dendritic-like Nanostructuresmentioning

confidence: 99%

Biosensor Applications of Electrodeposited Nanostructures

Stine

2019

Applied Sciences

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The development of biosensors for a range of analytes from small molecules to proteins to oligonucleotides is an intensely active field. Detection methods based on electrochemistry or on localized surface plasmon responses have advanced through using nanostructured electrodes prepared by electrodeposition, which is capable of preparing a wide range of different structures. Supported nanoparticles can be prepared by electrodeposition through applying fixed potentials, cycling potentials, and fixed current methods. Nanoparticle sizes, shapes, and surface densities can be controlled, and regular structures can be prepared by electrodeposition through templates. The incorporation of multiple nanomaterials into composite films can take advantage of the superior and potentially synergistic properties of each component. Nanostructured electrodes can provide supports for enzymes, antibodies, or oligonucleotides for creating sensors against many targets in areas such as genomic analysis, the detection of protein antigens, or the detection of small molecule metabolites. Detection can also be performed using electrochemical methods, and the nanostructured electrodes can greatly enhance electrochemical responses by carefully designed schemes. Biosensors based on electrodeposited nanostructures can contribute to the advancement of many goals in bioanalytical and clinical chemistry.

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“…[11,12] Among these, dendritic nanostructures, which were mostly used in the field of electrocatalysis to improve electrocatalytic effect performance, [13,14] have been reported to achieve high SERS effect because of the numbers of 'hot spots'. Moreover, bimetallic coordination enhancement, including Au-covered Ag, [15,16] Agcovered Au, [17,18] or Au and Ag alloys [19,20] ; Au-ZnO [21] ; Ag-TiO 2 or Au-TiO 2 ; [22] and Ag-covered C 70 , [23,24] has been proven to increase the enhancement effect compared with a single metal.…”

Section: Introductionmentioning

confidence: 99%

“…Different shapes and sizes of noble metal nanostructures (sphere, rod‐like, nanocup, dendritic, core‐shell, and so on) have been proposed to enhanced the Raman signals, such as physical sputtering or evaporation, or chemical synthesis of metal colloids . Among these, dendritic nanostructures, which were mostly used in the field of electrocatalysis to improve electrocatalytic effect performance, have been reported to achieve high SERS effect because of the numbers of ‘hot spots’. Moreover, bimetallic coordination enhancement, including Au‐covered Ag, Ag‐covered Au, or Au and Ag alloys; Au‐ZnO; Ag‐TiO 2 or Au‐TiO 2 ; and Ag‐covered C 70 , has been proven to increase the enhancement effect compared with a single metal …”

Section: Introductionmentioning

confidence: 99%

Electrodeposition of dendritic gold/silver nanaoparticles on disposable screen‐printed carbon electrode and its application of 4‐mercaptopyridine in in situ electrochemical surface‐enhanced Raman scattering

Teng

Ding

Liu

et al. 2016

Surface & Interface Analysis

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Column electrodes pretreated through oxidation-reduction cycles were traditionally used in electrochemical surface-enhanced Raman scattering (SERS). In this study, a disposable screen-printed carbon electrode was introduced into in situ electrochemical SERS through the electrodeposition of dendritic gold/silver nanoparticles (Au/AgNPs) onto the surface of the carbon working electrode to induce the SERS enhancement effect on the electrode. Scanning electron microscopy images showed that dendritic Au/AgNPs nanostructures could be fabricated under appropriate electrodeposition conditions and could present a minimum SERS factor of 4.25 × 10 5 . Furthermore, the absorbed behavior of 4-mercaptopyridine was investigated under different potentials. The adsorption configuration was inferred to transform from 'vertical' to 'lying-flat'. The proposed new electrode combined with a portable Raman spectrometer could be useful in the identifying products or intermediates during electrochemical synthesis or electrochemical catalysis in in situ electrochemical SERS.

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A sensor for glycoproteins based on dendritic gold nanoparticles electrodeposited on a gold electrode and modified with a phenylboronic acid

Cited by 9 publications

References 35 publications

Sensitive Glycoprotein Sandwich Assays by the Synergistic Effect of In Situ Generation of Raman Probes and Plasmonic Coupling of Ag Core–Au Satellite Nanostructures

Sensitive Glycoprotein Sandwich Assays by the Synergistic Effect of In Situ Generation of Raman Probes and Plasmonic Coupling of Ag Core–Au Satellite Nanostructures

Biosensor Applications of Electrodeposited Nanostructures

Electrodeposition of dendritic gold/silver nanaoparticles on disposable screen‐printed carbon electrode and its application of 4‐mercaptopyridine in in situ electrochemical surface‐enhanced Raman scattering

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