1997
DOI: 10.1016/s0925-4773(97)00038-5
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A sequence conserved in vertebrate Hox gene introns functions as an enhancer regulated by posterior homeotic genes in Drosophila imaginal discs

Abstract: The intron of the mouse Hoxa-4 gene acts as a strong homeotic response element in Drosophila melanogaster leg imaginal discs. This activity depends on homeodomain binding sites present within a 30 bp conserved element, HB1, in the intron. A similar arrangement of homeodomain binding sites is found in many other potential homeotic target genes. HB1 activity in Drosophila imaginal discs is activated by Antennapedia and more posterior homeotic genes, but is not activated by more anterior genes. Testing a reporter… Show more

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Cited by 23 publications
(16 citation statements)
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“…Furthermore, a relatively high stringency (100 bp, 70% sequence identity) was used in our comparison, which could have resulted in a bias towards the detection of ultraconserved elements with more highly conserved functions (such as the overall regulation of the HOX genes and the HOX clusters). Shorter, experimentally verified sequences, such as the enhancer in the intron of the HOXA4 gene (Keegan et al, 1997), were not detected in our analysis.…”
Section: Mapping Of Evolutionarily Conserved Regionsmentioning
confidence: 65%
“…Furthermore, a relatively high stringency (100 bp, 70% sequence identity) was used in our comparison, which could have resulted in a bias towards the detection of ultraconserved elements with more highly conserved functions (such as the overall regulation of the HOX genes and the HOX clusters). Shorter, experimentally verified sequences, such as the enhancer in the intron of the HOXA4 gene (Keegan et al, 1997), were not detected in our analysis.…”
Section: Mapping Of Evolutionarily Conserved Regionsmentioning
confidence: 65%
“…A transgene containing these sequences is expressed in the paraxial mesoderm with a boundary equivalent to that of Hoxa4 and deletion of a 2 kb fragment from the 5′ region abolishes reporter gene expression (Behringer et al, 1993). Using the same parent construct, mutation of Hox binding sites in the intron eliminates expression in the paraxial mesoderm and the posterior neural tube (Keegan et al, 1997). Thus, both the upstream region and the intron would seem to be required.…”
Section: Regulatory Organisation Of Pg-4 Hox Genesmentioning
confidence: 99%
“…In the mouse, Hoxc8 expression in the neural tube spans about six somite levels (from somites [10][11][12][13][14][15] and in the paraxial mesoderm about 7-8 somite levels (somites 13-21, with weaker levels of expression at somites 13 and 21). In the chick, Hoxc8 expression in the neural tube spans about six somite levels (somites 18-23; strong expression in somites [19][20] and in paraxial mesoderm about five somite levels (somites [21][22][23][24][25]. Thus Hoxc8 expression in paraxial mesoderm is 2-3 somite levels shorter in the chicken compared with mouse.…”
Section: Resultsmentioning
confidence: 97%
“…For retrograde labeling experiments, mouse (10.5 day postcoital) and chicken [Hamburger and Hamilton (H&H) 24] embryos were fixed in 4% paraformaldehyde in PBS. Brachial motor neurons were retrograde-labeled by placing finely ground 1,1Ј-dioctadecyl-3,3,3Ј,3Ј-tetramethylindocarbocyanine (DiI) crystals into one severed forelimb bud and a 1-to 2-week incubation in PBS, 10 mM EDTA.…”
Section: Methodsmentioning
confidence: 99%
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