A sequence‐tagged genetic map for the brown alga <i>Ectocarpus siliculosus</i> provides large‐scale assembly of the genome sequence

Heesch, Svenja; Cho, Ga Youn; Peters, Akira F.; Corguillé, Gildas Le; Falentin, Cyril; Boutet, Gilles; Coëdel, S.; Jubin, Claire; Samson, Gaëlle; Corre, Erwan; Coelho, Susana M.; Cock, J. Mark

doi:10.1111/j.1469-8137.2010.03273.x

Cited by 60 publications

(89 citation statements)

References 32 publications

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“…Nuclear DNA was amplified in a microsatellite region whose length differs by 40 bp between Ec32 (genetic background of the etl mutant) and Ec568 (Heesch et al, 2010; primer sequences are ESM27F, 59-TGTAAAACGGCCAGTGCAACTGCCCTCG-GAAAATAAG-39, and ESM27R, 59-GCGCGGTCGATGCTAATAATAA-CTA-39). The amplification was performed in an Applied Biosystems thermocycler (35 cycles, 0.5 min 948C, 0.5 min 658C, 1 min 728C) using GoTaq DNA polymerase (Promega).…”

Section: Cross-verificationmentioning

confidence: 99%

ETOILERegulates Developmental Patterning in the Filamentous Brown AlgaEctocarpus siliculosus

et al. 2011

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Brown algae are multicellular marine organisms evolutionarily distant from both metazoans and land plants. The molecular or cellular mechanisms that govern the developmental patterning in brown algae are poorly characterized. Here, we report the first morphogenetic mutant, e´toile (etl), produced in the brown algal model Ectocarpus siliculosus. Genetic, cellular, and morphometric analyses showed that a single recessive locus, ETL, regulates cell differentiation: etl cells display thickening of the extracellular matrix (ECM), and the elongated, apical, and actively dividing E cells are underrepresented. As a result of this defect, the overrepresentation of round, branch-initiating R cells in the etl mutant leads to the rapid induction of the branching process at the expense of the uniaxial growth in the primary filament. Computational modeling allowed the simulation of the etl mutant phenotype by including a modified response to the neighborhood information in the division rules used to specify wild-type development. Microarray experiments supported the hypothesis of a defect in cell-cell communication, as primarily Lin-Notch-domain transmembrane proteins, which share similarities with metazoan Notch proteins involved in binary cell differentiation were repressed in etl. Thus, our study highlights the role of the ECM and of novel transmembrane proteins in cell-cell communication during the establishment of the developmental pattern in this brown alga.

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Section: Cross-verificationmentioning

confidence: 99%

ETOILERegulates Developmental Patterning in the Filamentous Brown AlgaEctocarpus siliculosus

et al. 2011

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“…Despite this situation, first reports on the characterization of natural (Peters et al, 2008) and artificial mutants (Coelho et al, 2011;Lebail et al, 2010Lebail et al, , 2011, altered in their development pattern, have been published. The availability of a genetic map generated recently for the Ectocarpus reference strain (Heesch et al, 2010) represents a valuable tool for further genetic analysis.…”

Section: Discussionmentioning

confidence: 99%

Toward Systems Biology in Brown Algae to Explore Acclimation and Adaptation to the Shore Environment

Tonon

Eveillard

Prigent

et al. 2011

OMICS: A Journal of Integrative Biology

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Brown algae belong to a phylogenetic lineage distantly related to land plants and animals. They are almost exclusively found in the intertidal zone, a harsh and frequently changing environment where organisms are submitted to marine and terrestrial constraints. In relation with their unique evolutionary history and their habitat, they feature several peculiarities, including at the level of their primary and secondary metabolism. The establishment of Ectocarpus siliculosus as a model organism for brown algae has represented a framework in which several omics techniques have been developed, in particular, to study the response of these organisms to abiotic stresses. With the recent publication of medium to high throughput profiling data, it is now possible to envision integrating observations at the cellular scale to apply systems biology approaches. As a first step, we propose a protocol focusing on integrating heterogeneous knowledge gained on brown algal metabolism. The resulting abstraction of the system will then help understanding how brown algae cope with changes in abiotic parameters within their unique habitat, and to decipher some of the mechanisms underlying their (1) acclimation and (2) adaptation, respectively consequences of (1) the behavior or (2) the topology of the system resulting from the integrative approach.

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“…isolated from independently micro-dissected unilocular sporangia). Initially, 30 individuals from this segregating population were genotyped with 97 microsatellite markers distributed at ∼30 cM intervals along the length of the entire genetic map (Heesch et al, 2010). Additional microsatellite markers were developed based on the Ec32 genome sequence to fine map the mutant locus (Table S1).…”

Section: Genetic Mapping Of the Imm Locusmentioning

confidence: 99%

“…Peters et al (2008) showed that the imm mutation behaved as a recessive, Mendelian allele and was located on an autosome. To map this mutation, a backcrossed descendant of strain Ec137 carrying the imm mutation (Peters et al, 2008) was crossed with the outcrossing line Ec568 (Heesch et al, 2010) to generate a segregating family of 1699 haploid progeny. The IMM locus was then mapped genetically by scanning the genome for linked microsatellite markers and fine mapping the mutation.…”

Section: Introductionmentioning

confidence: 99%

“…The IMM locus was then mapped genetically by scanning the genome for linked microsatellite markers and fine mapping the mutation. To scan for linked markers, a subset of the population (30 to 75 individuals) was genotyped for 97 microsatellite markers (Heesch et al, 2010) distributed at ∼30 cM intervals along the length of the entire genetic map. Additional markers were then generated for a region on chromosome 27 that exhibited co-segregation with the Imm + phenotype and these were tested against the entire population to fine map the IMM locus.…”

Section: Introductionmentioning

confidence: 99%

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The Ectocarpus IMMEDIATE UPRIGHT gene encodes a member of a novel family of cysteine-rich proteins with an unusual distribution across the eukaryotes

et al. 2017

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The sporophyte generation of the brown alga Ectocarpus sp. exhibits an unusual pattern of development compared with the majority of brown algae. The first cell division is symmetrical and the apical-basal axis is established late in development. In the immediate upright (imm) mutant, the initial cell undergoes an asymmetric division to immediately establish the apical-basal axis. We provide evidence which suggests that this phenotype corresponds to the ancestral state of the sporophyte. The IMM gene encodes a protein of unknown function that contains a repeated motif also found in the EsV-1-7 gene of the Ectocarpus virus EsV-1. Brown algae possess large families of EsV-1-7 domain genes but these genes are rare in other stramenopiles, suggesting that the expansion of this family might have been linked with the emergence of multicellular complexity. EsV-1-7 domain genes have a patchy distribution across eukaryotic supergroups and occur in several viral genomes, suggesting possible horizontal transfer during eukaryote evolution.

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A sequence‐tagged genetic map for the brown alga Ectocarpus siliculosus provides large‐scale assembly of the genome sequence

Cited by 60 publications

References 32 publications

ETOILERegulates Developmental Patterning in the Filamentous Brown AlgaEctocarpus siliculosus

ETOILERegulates Developmental Patterning in the Filamentous Brown AlgaEctocarpus siliculosus

Toward Systems Biology in Brown Algae to Explore Acclimation and Adaptation to the Shore Environment

The Ectocarpus IMMEDIATE UPRIGHT gene encodes a member of a novel family of cysteine-rich proteins with an unusual distribution across the eukaryotes

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