A sequential 3D bioprinting and orthogonal bioconjugation approach for precision tissue engineering

Yu, Claire; Miller, Kathleen L.; Schimelman, Jacob; Wang, Pengrui; Zhu, Wei; Ma, Xinwei; Tang, Min; You, Shangting; Lakshmipathy, Deepak R.; He, Frank; Chen, Shaochen

doi:10.1016/j.biomaterials.2020.120294

Cited by 44 publications

(48 citation statements)

References 46 publications

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“…Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are superior to primary cardiomyocytes as they can expand prior to differentiation to cardiomyocytes and also are easy to scale up (Zuppinger, 2019). GelMA-a photopolymerizable ECM has adhesion moieties and natural degradation making it a popular choice for printing encapsulated cells (Yu et al, 2020). To design hiPSC-CMs models, cells have been encapsulated in ECM, Fibrin or gelatin methacrylate (GelMA) (Veldhuizen et al, 2019) to facilitate the formation of connections in 3D space (Ma et al, 2018).…”

Section: Hipsc-derived Cardiomyocytesmentioning

confidence: 99%

3D Bioprinting Technology – One Step Closer Towards Cardiac Tissue Regeneration

Chingale

Cheng

2022

Front. Mater.

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Cardiovascular diseases are one of the leading causes of death across the globe. Heart transplantation has been used for end stage heart failure patients. However, due to the lack of donors, this treatment option usually depends on multiple variables and the result varies due to immunological issues. 3D bioprinting is an emerging approach for in vitro generation of functional cardiac tissues for drug screening and cardiac regenerative therapy. There are different techniques such as extrusion, inkjet, or laser-based 3D printing that integrate multiple cell lines with different scaffolds for the construction of complex 3D structures. In this review, we discussed the recent progress and challenges in 3D bioprinting strategies for cardiac tissue engineering, including cardiac patches, in vitro cardiac models, valves, and blood vessels.

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Section: Hipsc-derived Cardiomyocytesmentioning

confidence: 99%

3D Bioprinting Technology – One Step Closer Towards Cardiac Tissue Regeneration

Chingale

Cheng

2022

Front. Mater.

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“…Light-based approaches, such as two-photon polymerisation, allow better resolution on the micro to nanoscale; however often require transparent materials [105][106][107]. Photopatterning is also a well-established method of patterning biologically active growth factors, proteins and peptides into hydrogel scaffolds [99,108]. Microfluidics enable creation of devices with microchannels and segregated compartments [54,61]; as well as enabling tight control over microscale features via precise biochemical patterning of organoids and hydrogel scaffolds [48,99].…”

Section: Topographical Patterningmentioning

confidence: 99%

“…Traditional methods of incorporating biochemical or mechanical cues take an ‘all or nothing' approach, failing to account for interconnectedness of variables and synergy of cues such as microtopographies and biochemical gradients [ 111 , 112 ]. Researchers are beginning to recognise the power of combining various approaches to create highly versatile and dynamic culture systems [ 54 , 61 , 108 ]. Such advanced systems are capable of recreating some of the compositional and architectural complexity of in vivo tissues, whilst also providing insight into the power of interconnected environmental cues on cell behaviour.…”

Section: Biomaterialsmentioning

confidence: 99%

Modelling the central nervous system: tissue engineering of the cellular microenvironment

Walczak

Esteban

Bassett

et al. 2021

Emerging Topics in Life Sciences

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With the increasing prevalence of neurodegenerative diseases, improved models of the central nervous system (CNS) will improve our understanding of neurophysiology and pathogenesis, whilst enabling exploration of novel therapeutics. Studies of brain physiology have largely been carried out using in vivo models, ex vivo brain slices or primary cell culture from rodents. Whilst these models have provided great insight into complex interactions between brain cell types, key differences remain between human and rodent brains, such as degree of cortical complexity. Unfortunately, comparative models of human brain tissue are lacking. The development of induced Pluripotent Stem Cells (iPSCs) has accelerated advancement within the field of in vitro tissue modelling. However, despite generating accurate cellular representations of cortical development and disease, two-dimensional (2D) iPSC-derived cultures lack an entire dimension of environmental information on structure, migration, polarity, neuronal circuitry and spatiotemporal organisation of cells. As such, researchers look to tissue engineering in order to develop advanced biomaterials and culture systems capable of providing necessary cues for guiding cell fates, to construct in vitro model systems with increased biological relevance. This review highlights experimental methods for engineering of in vitro culture systems to recapitulate the complexity of the CNS with consideration given to previously unexploited biophysical cues within the cellular microenvironment.

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“…3D bioprinting has emerged as an intriguing approach for the production of complex in vitro models, by which means cells and/or their supporting scaffold are precisely deposited, localized, or joined in user-defined geometries and dimensions. With an ever-expanding range of available biomaterials (Yu et al 2020a , b ) and biocompatible processes (Ashammakhi et al 2019 ), 3D bioprinting has aided in the tailored control over microarchitecture, extracellular matrix (ECM) construction, and cell deposition for the establishment of in vitro models, particularly the recapitulation of complex tissues (Ma et al 2018a , b ), and has resulted in significant accomplishments in moving the field forward in recent years.…”

Section: Introductionmentioning

confidence: 99%

3D bioprinting of complex tissues in vitro: state-of-the-art and future perspectives

et al. 2022

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The pharmacology and toxicology of a broad variety of therapies and chemicals have significantly improved with the aid of the increasing in vitro models of complex human tissues. Offering versatile and precise control over the cell population, extracellular matrix (ECM) deposition, dynamic microenvironment, and sophisticated microarchitecture, which is desired for the in vitro modeling of complex tissues, 3D bio-printing is a rapidly growing technology to be employed in the field. In this review, we will discuss the recent advancement of printing techniques and bio-ink sources, which have been spurred on by the increasing demand for modeling tactics and have facilitated the development of the refined tissue models as well as the modeling strategies, followed by a state-of-the-art update on the specialized work on cancer, heart, muscle and liver. In the end, the toxicological modeling strategies, substantial challenges, and future perspectives for 3D printed tissue models were explored.

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A sequential 3D bioprinting and orthogonal bioconjugation approach for precision tissue engineering

Cited by 44 publications

References 46 publications

3D Bioprinting Technology – One Step Closer Towards Cardiac Tissue Regeneration

3D Bioprinting Technology – One Step Closer Towards Cardiac Tissue Regeneration

Modelling the central nervous system: tissue engineering of the cellular microenvironment

3D bioprinting of complex tissues in vitro: state-of-the-art and future perspectives

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