A sequential approach using genetic and morphological analyses to test species status: The case of United States federally endangered <i>Agalinis acuta</i> (Orobanchaceae)

Pettengill, James B.; Neel, Maile C.

doi:10.3732/ajb.1000267

Cited by 8 publications

(2 citation statements)

References 55 publications

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“…Funk & Omland (2003) found that non‐monophyletic species were found in 23% of cases in a study of more than 2000 species of animals. Many cases of non‐monophyletic species have also been detected in plants (e.g., Fazekas & al., 2008; Pettengill & Neel, 2010; Arca & al., 2012). According to our identifications, Micropholis egensis and M. madeirensis were polyphyletic, and M. guyanensis and M. venulosa were both polyphyletic and paraphyletic in the three analyses.…”

Section: Discussionmentioning

confidence: 99%

A plea to DNA barcode type specimens: An example fromMicropholis(Sapotaceae)

Sánchez‐C.

Richardson

Hart

et al. 2021

TAXON

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Identification of individuals in the absence of reproductive traits or in juvenile stages can be difficult. Incorrect identification will result in inaccurate biodiversity inventories that are an impediment to effective conservation management. Here, we present data in which we argue that the generation of DNA barcode reference libraries using DNA extracted from type specimens could resolve issues related to correct identification. The genus Micropholis (Sapotaceae) is diverse and ecologically important in Neotropical lowland rain forests. We first assessed the capacity of the molecular markers ITS2, matK and rbcL in differentiating species in the genus. Based on a phylogenetic reconstruction we found that many individuals in our study were incorrectly identified, and we argue that if we had access to a type specimen DNA barcode reference library we would have more rapidly and correctly identified individuals.

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Section: Discussionmentioning

confidence: 99%

A plea to DNA barcode type specimens: An example fromMicropholis(Sapotaceae)

Sánchez‐C.

Richardson

Hart

et al. 2021

TAXON

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“…Therefore, the current phylogenetic analyses reflected a clear separation of the sampled individuals into two distinct clades, which also presented distinct morphological traits (see introduction). A growing number of studies have argued that concordance among independent sources of molecular data can be a criterion for identifying distinct species (Groeneveld, Weisrock, Rasoloarison, Yoder, & Kappeler, ; Leavitt, Johnson, & St. Clair, ; Pettengill & Neel, ; Weisrock et al, ). In our study, the concordance of divergence between morphological, mitochondrial locus and nuclear DNA analyses should be viewed as the most convincing evidence supporting P. gravivox and P. swinhoei as distinct species.…”

Section: Discussionmentioning

confidence: 99%

Morphotypes or distinct species? A multilocus assessment of two East Asian scimitar babblers (Aves, Timaliidae)

2020

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In birds, widespread species complexes often exhibit dramatic plumage differences across their distribution, which can give rise to discordance between morphotypes and phylogroups. Accurate identification of species diversity may require an integrated approach in which multilocus genetic data are used for inference and further corroborated by ecological, morphometric or behavioural data. Pomatorhinus gravivox and P. swinhoei, which were formerly considered component races of P. erythrogenys, were recently split using a delimitation system that mainly relied on the quantification of differences in phenotypic traits. We therefore carried out a reassessment of this taxonomic recommendation by conducting phylogenetic and population genetic analyses with multilocus genetic data. A deep mitochondrial split with a Kimura 2-parameter distance of 0.061 was observed, mainly corresponding to the two morphologically defined species. However, one individual from P. swinhoei harboured a haplotype of P. gravivox. Individual-based analyses of nuclear loci identified two distinct clusters that were exactly congruent with the two species. BPP delimitation also provided support for the separation. These congruencies support the notion that these two taxa are best regarded as two separate species. The presence of the P. gravivox mitochondrial haplotype in P. swinhoei was most likely a result of hybridization, due to the clear separation of nuclear loci. The speciation might be attributed to paleoclimatic changes but requires further evaluation due to the likelihood of ecological speciation. This study was in accordance with the results inferred from the quantitative system but highlighted the importance of sampling various data, especially in contact zones, in the study of taxonomy and speciation history. K E Y W O R D Sgenetic divergence, hybridization, morphotypes, speciation 266 | DAI et Al.

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