A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents

LPS Triggers Acute Neuroinflammation and Parkinsonism Involving NLRP3 Inflammasome Pathway and Mitochondrial CI Dysfunction in the Rat

“…Four independent motor behavior tests showed the physiological deficit of the LPSinduced dopaminergic nigrostriatal neurodegeneration [37]: the beam walking test to assess motor coordination, the balance and the limb-use asymmetry ("cylinder") test to evaluate locomotor asymmetry, the vibrissae-evoked forelimb placing test to assess asymmetry in the sensorimotor cortex and striatum, and the open field test to analyze changes in locomotion. The evaluations were carried out in four groups of rats: (1) untreated, (2) Mock, and at (3) 15 and (4) 30 days after LPS administration (Figure 9).…”

“…The beam walking test evaluated the balance, motor coordination, and traveled time while rodents crossed a narrow beam (1 cm wide and 2 m long, placed at 45 • tilt) [37]. The test was carried out after two days of pre-training.…”

“…The cylinder test evaluated the locomotor asymmetry of the forelimbs when they touched the walls of a transparent acrylic cylinder (30 cm tall, 20 cm diameter) [37]. First, the behavior was video recorded to evaluate the first 20 contacts made with the ipsilateral, the contralateral, and both paws simultaneously [67].…”

Detection of Brain Abnormalities in Parkinson’s Rats by Combining Deep Learning and Motion Tracking

“…Local injection into the rat brain can damage the dopaminergic neurons in the substantia nigra striatum pathway in the rat brain and reduce the secretion of dopamine, resulting in motor and nonmotor defects. Therefore, in clinical research on Parkinson's disease, the animal model of Parkinson's disease that contributes the most is undoubtedly the unilateral MFB-injected 6-OHDAinjured rat [9]. Therefore, in this research, 12 adult male Sprague-Dawley rats (starting body weight of 250-300g) were divided into two groups: 6 rats with medial forebrain bundle (MFB) injury were selected as the experimental group and 6 normal rats were used as the control group.…”

“…The introduction of 6-OHDA caused damage to dopaminergic neurons of the nigrostriatal pathway in the rat brain and reduced dopamine secretion, resulting in motor and nonmotor deficits. Researchers have developed a variety of methods for the behavioural assessment of Parkinson's disease rats, such as the cylinder test, gait adjustment test, olfactory asymmetry test, and forced swim test [9], [10], [11].…”