A Sequential Statistical Approach towards an Optimized Production of a Broad Spectrum Bacteriocin Substance from a Soil Bacterium<i>Bacillus</i>sp. YAS 1 Strain

Embaby, Amira M.; Heshmat, Yasmin; Hussein, Ahmed; Marey, H. S.

doi:10.1155/2014/396304

Cited by 16 publications

(23 citation statements)

References 40 publications

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“…These designs have been employed for maximizing the yield of enzymes, soluble proteins, amino acids, antimicrobial agents, etc [31,34,35] instead of the traditional methods (one variable at a time).…”

Section: Discussionmentioning

confidence: 99%

A Novel Non-Cumbersome Approach Towards Biosynthesis of Pectic-Oligosaccharides by Non-Aflatoxigenic Aspergillus sp. Section Flavi Strain EGY1 DSM 101520 through Citrus Pectin Fermentation

et al. 2016

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Pectic-Oligosaccharides (POS) have a growing potential in food and feed industries. To satisfy the demand of worldwide markets from POS and avoid the shortcomings of currently applied methodologies encountered in their preparation, the present study highlights a novel robust approach for POS biosynthesis. In the current approach, Aspergillus sp.section Flavi strain EGY1 DSM 101520 was grown on citrus pectin-based medium as a core POS production medium. POS' levels accumulated in the fungal fermentation broth were optimized through a three step sequential statistical mathematical methodology; Plackett-Burman design (PBD), Box-Behnken design (BBD) and canonical analysis. Three key determinants namely citrus pectin, peptone and NaH2PO4 were pointed out by PBD to impose significant consequences (P<0.05) on the process outcome (POS' levels). Optimal levels of these key determinants along with maximal of POS' levels were set by BBD and canonical analysis to be 2.28% (w/v) citrus pectin, 0.026% (w/v) peptone and 0.28% (w/v) NaH2PO4 to achieve a net amount of 1.3 g POS /2.28 g citrus pectin. Through this approach, a yield of 57% (w/w) POS of the total citrus pectin was obtained after 24 h of fungal growth on optimized citrus pectin–based medium. A fold enhancement of 13 times in POS' levels released in the fermentation fungal broth was realized by the end of the optimization strategy. This novel robust approach is considered a new insight towards POS biosynthesis via efficient, rapid and non-cumbersome procedure. To the best of authors' knowledge, the present work is the first article underlining detailed POS production from the fermentation broth of a fungus growing on citrus pectin-based medium.

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Section: Discussionmentioning

confidence: 99%

A Novel Non-Cumbersome Approach Towards Biosynthesis of Pectic-Oligosaccharides by Non-Aflatoxigenic Aspergillus sp. Section Flavi Strain EGY1 DSM 101520 through Citrus Pectin Fermentation

et al. 2016

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“…Maximum BLIS An6 was successfully achieved after 48-h fermentation in a production medium consisted of starch (20 g/L) and yeast extract (10 g/L) (Ayed et al 2015). In RSM-optimized condition, yeast extract exerts significant effect on bacteriocin BAC YAS1 and XH25 level, but the former was not influenced by soluble starch and initial pH (Embaby et al 2014). In an experiment involving several media components [e.g.…”

Section: Carbon and Nitrogen Sourcesmentioning

confidence: 99%

“…In RSM-optimized fermentation condition, it was found that highest bacteriocin HJDA32 could be obtained using inocula size of 4% at pH 5.3, agitation rate of 147 rpm and temperature of 30 °C for 30 h (Hu et al 2014). Sequential statistical approach using Plackett-Burman coupled with RSM (Box-Behnken) was successfully employed whereby optimization of key variables [yeast extract (0.48% (w/v), fermentation time (62 h) and agitation rate (207 rpm)] in peptone-yeast-beef based medium enhanced 1.6-fold BAC YAS 1 production (Embaby et al 2014). In RSM (2 3 factorial design), three determinants chosen [temperature (26-37 °C), initial pH (6.5-7.5) and whey concentration (70 g/L)] showed maximum bacteriocin production at 15 h fermentation time (Cladera-Olivera et al 2004).…”

Section: Fermentation Timementioning

confidence: 99%

Biomanufacturing process for the production of bacteriocins from Bacillaceae family

Lajis

2020

Bioresour. Bioprocess.

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Members of Bacillaceae family are of major interest in medical industry due to vast antimicrobial peptides they produce as therapeutic agents. For decades, synthetic and natural occurring antibiotics have been used to treat infectious diseases, but heavy dependence on these drugs has led to significant drawbacks which propel continuous development of new antibiotics generation. Recent findings have shown several bacteriocins of Bacillaceae as promising alternatives to the conventional drugs to combat the emergence of new drug-resistant pathogens. In this present review, Bacillaceae bacteriocins' classification such as lantibiotics and thiazole/oxazole-modified microcins as well as their biochemical characterization such as sensitivity to enzymes, temperature, pH and chemicals are described. This article enlightens on the medical application of several Bacillaceae bacteriocins emphasizing those that underwent and ongoing preclinical trials. This review also discusses the development of Bacillaceae bacteriocins production, focusing strains selection and fermentation factors such as inocula size, medium (carbon, nitrogen, minerals sources), temperature, pH, agitation and aeration rate, dissolved oxygen tension (DOT), fermentation time, inducers and mode of operation via various statistical methods for their optimization. It also highlights recent advance in the production of bioengineered and recombinant bacteriocins in bioreactors system which are rarely disclosed in literature.

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“…YAS 1 could be increased 1.6-fold by this method. This bacteriocin had a wide pH range (1–13) as well as temperature (45–80°C) with antimicrobial activity to human pathogens such as Clostridium , Staphylococcus , Enterococcus , and Salmonella , and plant pathogens such as E. amylovora , and showing no effect on lactic acid bacteria ( Embaby et al, 2014 ).…”

Section: Bacteriocins In the Veterinary Industry And Human Medicinementioning

confidence: 99%

Bacteriocins from the rhizosphere microbiome – from an agriculture perspective

Subramanian

Smith

2015

Front. Plant Sci.

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Bacteria produce and excrete a versatile and dynamic suit of compounds to defend against microbial competitors and mediate local population dynamics. These include a wide range of broad-spectrum non-ribosomally synthesized antibiotics, lytic enzymes, metabolic by-products, proteinaceous exotoxins, and ribosomally produced antimicrobial peptides (bacteriocins). Most bacteria produce at least one bacteriocin. Bacteriocins are of interest in the food industry as natural preservatives and in the probiotics industry, leading to extensive studies on lactic acid bacteria (colicin produced by Escherichia coli is a model bacteriocin). Recent studies have projected use of bacteriocins in veterinary medicine and in agriculture, as biostimulants of plant growth and development and as biocontrol agents. For example, bacteriocins such as Cerein 8A, Bac-GM17, putidacin, Bac 14B, amylocyclicin have been studied for their mechanisms of anti-microbial activity. Bac IH7 promotes tomato and musk melon plant growth. Thuricin 17 (Th17) is the only bacteriocin studied extensively for plant growth promotion, including at the molecular level. Th17 functions as a bacterial signal compound, promoting plant growth in legumes and non-legumes. In Arabidopsis thaliana and Glycine max Th17 increased phytohormones IAA and SA at 24 h post treatment. At the proteome level Th17 treatment of 3-week-old A. thaliana rosettes led to >2-fold changes in activation of the carbon and energy metabolism pathway proteins, 24 h post treatment. At 250 mM NaCl stress, the control plants under osmotic-shock shut down most of carbon-metabolism and activated energy-metabolism and antioxidant pathways. Th17 treated plants, at 250 mM NaCl, retained meaningful levels of the light harvesting complex, photosystems I and II proteins and energy and antioxidant pathways were activated, so that rosettes could better withstand the salt stress. In Glycine max, Th17 helped seeds germinate in the presence of NaCl stress, and was most effective at 100 mM NaCl. The 48 h post germination proteome suggested efficient and speedier partitioning of storage proteins, activation of carbon, nitrogen and energy metabolisms in Th17 treated seeds both under optimal and 100 mM NaCl. This review focuses on the bacteriocins produced by plant-rhizosphere colonizers and plant-pathogenic bacteria, that might have uses in agriculture, veterinary, and human medicine.

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A Sequential Statistical Approach towards an Optimized Production of a Broad Spectrum Bacteriocin Substance from a Soil BacteriumBacillussp. YAS 1 Strain

Cited by 16 publications

References 40 publications

A Novel Non-Cumbersome Approach Towards Biosynthesis of Pectic-Oligosaccharides by Non-Aflatoxigenic Aspergillus sp. Section Flavi Strain EGY1 DSM 101520 through Citrus Pectin Fermentation

A Novel Non-Cumbersome Approach Towards Biosynthesis of Pectic-Oligosaccharides by Non-Aflatoxigenic Aspergillus sp. Section Flavi Strain EGY1 DSM 101520 through Citrus Pectin Fermentation

Biomanufacturing process for the production of bacteriocins from Bacillaceae family

Bacteriocins from the rhizosphere microbiome – from an agriculture perspective

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