A serine proteinase from the sarcoplasmic fraction of red sea bream Pagrus major is possibly derived from blood

Abstract

Collagen degradation is known to be involved in post mortem tenderization of fish muscle. A serine proteinase which is assumed to be related to collagen degradation after fish death was purified from sarcoplasmic fraction of red sea bream Pagrus major by ammonium sulfate fractionation and column chromatographies on Sephacryl S-300, Q Sepharose and Phenyl Sepharose CL-4B. The enzyme hydrolyzed gelatin and was obtained as a protein band of approximately 38 kDa on sodium dodecyl sulfate polyacrylamide gel electrophoresis under reducing condition. N-terminal amino acid sequence of the enzyme was determined for 32 residues. The protein having the same N-terminal amino acid sequence of the enzyme for 10 residues was purified from the serum of red sea bream and showed the same characteristics as the enzyme. Therefore, the serine proteinase was suggested to migrate from blood to muscle and to degrade muscle proteins after fish death.

PARTIAL CHARACTERIZATION OF GELATINOLYTIC PROTEINASES FROM THE SKELETAL MUSCLE OF GRASS CARP (CTENOPHARYNGODON IDELLUS)

“…Gelatinolytic proteinases were detected in red sea bream muscle, gelatinolytic proteinase at 85 kDa was serine proteinase, but two gelatinolytic enzymes at 55 and 52 kDa were metalloproteinases (Wu et al 2010a,b). Yoshida et al (2009) reported that a gelatinolytic serine proteinase was 38 kDa. We also found two gelatinolytic proteinases (90 and 70 kDa) in common carp muscle.…”

“…Gelatinolytic serine proteinases were detected in many fish species by gelatin zymography, including red stingray wing ( Dasyatis akajei ) (Bae et al . 2010), red sea bream ( Pagrus major ) (Yoshida et al . 2009; Wu et al .…”

“…(Bracho and Haard 1995), muscles of ayu (Plecoglossus altivelis) (Kubota et al 2000), Atlantic cod (Gadus morhua), spotted wolfish (Anarhichas minor) and Atlantic salmon (Salmo salar) (Lødemel and Olsen 2003). Gelatinolytic serine proteinases were detected in many fish species by gelatin zymography, including red stingray wing (Dasyatis akajei) (Bae et al 2010), red sea bream (Pagrus major) (Yoshida et al 2009;Wu et al 2010a), ayu (Plecoglossus altivelis) (Kubota et al 2000) and so on.…”