A serological comparison of bovine coronavirus strains

Abstract

Two bovine coronavirus (BCV) strains from diarrheic calf faeces were adapted to grow in HRT 18 cells and compared in immunofluorescence (IF), haemagglutination inhibition (HAI) and neutralisation (NT) tests with three other strains of BCV and a human coronavirus (HCV) strain obtained from other laboratories. Polyclonal antisera against these 6 viruses were raised in rabbits. No significant differences between viruses were detected by IF. In the HAI test the HCV strain was distinguishable from the 5 BCV strains and serological variation between the BCV strains was shown. HCV could be distinguished by NT test, but all BCV isolates were similar. Two monoclonal antibodies prepared against one of the BCV strains distinguished HCV in all three assays, and detected varying relationships between BCV strains.

Field Isolates of Transmissible Gastroenteritis Virus Differ at the Molecular Level from the Miller and Purdue Virulent and Attenuated Strains and from Porcine Respiratory Coronaviruses.

“…Some correlation was observed between the Sau3AI groups defined in the present study and MAb defined groups described previously [41]. Antigenic variation among other coronaviruses, such as infectious bronchitis virus (IBV) [12,24], bovine coronavirus [7], and feline coronavirus [8] has been observed. Kwon et al [23] used PCR/RFLP analysis to differentiate IBV strains.…”

Sero-prevalence, cross-species infection and serological determinants of prevalence of Bovine Coronavirus in Cattle, Sheep and Goats in Ghana

“…1016 T genus Betacoronavirus (Yang and Leibowitz, 2015;Oma et al, 2016;Pfefferle et al, 2009). While different strains may have some antigenic variability, all strains elicit cross-reactive seropositivity and thus form a single serotype (Clark, 1993;El-Ghorr et al, 1989). The virus is an important livestock pathogen causing effects on animal welfare as well as the economy (Lathrop et al, 2000a).…”

Bovine coronaviruses associated with enteric and respiratory diseases in Canadian dairy cattle display different reactivities to anti-HE monoclonal antibodies and distinct amino acid changes in their HE, S and ns4.9 protein

“…Despite their association to different clinical pathologies, BCoV isolates apparently belong to a single serotype on the basis of virus neutralisation (VN) and hemagglutination inhibition (HAI) tests using polyclonal hyperimmune sera. However, data obtained from recent serological findings using anti-HE and anti-S MAbs suggest the existence of different subgroups of BCoV, each containing EBCoV and WDBCoV strains responsible for either NCD, WD or chronic shedding in adult cattle (El Ghorr et al, 1989;Tsunemitsu and Saif, 1995;Milane et al, 1997;Fukutomi et al, 1999). Antigenic and genomic variations have been also recently reported among EBCoV and RBCoV strains (Chouljenko et al, 1998;Hasoksuz et al, 1999).…”