A Serotype VIII Strain among Colonizing Group B Streptococcal Isolates in Boston, Massachusetts

Paoletti, Leanne J.; Bradford, Jessica; Paoletti, Lawrence C.

doi:10.1128/jcm.37.11.3759-3760.1999

Cited by 23 publications

(9 citation statements)

References 13 publications

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“…Disc diffusion testing was also performed with erythromycin (15 mg) and clindamycin (2 mg) discs, following NCCLS guidelines. Capsule serotyping was performed using serological methods and standard reference antisera (Paoletti et al, 1999). Typing for the major GBS surface C proteins was performed using PCR methods and primers designed to detect unique regions of the genes for the alpha C protein, Alp1, Alp2, Alp3, Rib and beta C protein (L. C. Madoff, unpublished results).…”

Section: Methodsmentioning

confidence: 99%

A composite transposon associated with erythromycin and clindamycin resistance in group B Streptococcus

Puopolo

Klinzing

Lin

et al. 2007

Journal of Medical Microbiology

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Group B Streptococcus (GBS) resistant to erythromycin and clindamycin has been isolated with increasing frequency since the mid-1990s. This work studied GBS isolates from three US cities to determine the genetic basis of the macrolide resistance phenotype. ermB genes were amplified from five isolates collected in Boston, Pittsburgh and Seattle from infant and adult sources. Gene-walking methods were used to determine the chromosomal location of ermB and to identify associated genes. Southern mapping and random amplified polymorphic DNA (RAPD) analyses were used to distinguish the isolates. The ermB gene was present on the chromosome within a composite Tn917/Tn916-like transposon similar to one identified in Streptococcus pneumoniae. Four strains from Boston and Pittsburgh were serotype V and identical by Southern hybridization and RAPD analysis. The Seattle isolate was serotype Ib, with different patterns on RAPD analysis and Southern mapping. The composite transposon was integrated at an identical chromosomal site in all five isolates. The presence of this composite transposon in both GBS and pneumococci suggests that ermB-mediated macrolide resistance in streptococci may be due to the horizontal transfer of a mobile transposable element, and raises concern for further dissemination of high-grade erythromycin and clindamycin resistance among streptococcal species.

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Section: Methodsmentioning

confidence: 99%

A composite transposon associated with erythromycin and clindamycin resistance in group B Streptococcus

Puopolo

Klinzing

Lin

et al. 2007

Journal of Medical Microbiology

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“…In Denmark, seven instances of invasive disease isolates belonging to CPS VIII among nonpregnant adults were reported from 1999 to 2002 (51). Also, one isolate colonizing a pregnant woman in Maryland was recognized as CPS VIII (52). Lately, the frequency of CPS VIII is generally low outside Japan, and studies often describe few isolates (53,54).…”

Section: Serotypingmentioning

confidence: 99%

“…One study assessed a collection of GBS isolates classified as NT by serological methods and went on to characterize them by a number of different techniques (immunodiffusion, pulsed-field gel electrophoresis [PFGE], and multilocus sequence typing) (59). This resulted in all isolates being serotyped and found to represent all types, excluding IV and VII to IX, with instances of multiple serotypes per isolate, which has been described previously (52). This may represent an incidence of capsular switching, a phenomenon that involves the genetic exchange of capsular genes and thus enables expression of alternative capsule types, and it is proposed that such horizontal transfer may be responsible for the capsular locus diversity (60)(61)(62).…”

Section: Serotypingmentioning

confidence: 99%

Perinatal Streptococcus agalactiae Epidemiology and Surveillance Targets

2018

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SUMMARY, or group B streptococcus (GBS), is a major neonatal pathogen. Recent data have elucidated the global prevalence of maternal and neonatal colonization, but gaps still remain in the epidemiology of this species. A number of phenotypic and genotypic classifications can be used to identify the diversity of GBS strains, and some are more discriminatory than others. This review explores the main schemes used for GBS epidemiology and further details the targets for epidemiological surveillance. Current screening practices across the world provide a unique opportunity to gain detailed information on maternal colonizing strains and neonatal disease-causing strains, which is vital for monitoring and therapeutics, if sufficient detail can be extracted. Deciphering which isolates are circulating within specific populations and recording targets within invasive strains are crucial steps in monitoring the implementation of therapeutics, such as vaccines, as well as developing novel therapies against prevalent GBS strains. Having a detailed understanding of global GBS epidemiology will prove invaluable for understanding the pathogenesis of this organism and equipping future prevention strategies for success.

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“…Studies from neighboring regions, such as the United Arab Emirates, revealed a predominance of serotype IV followed by types Ia, III and V (17), while Kuwait had similar serotype distribution to our population (serotype III: 24.3%, serotype V: 21.8%, serotype Ia: 12.9%, serotypes II and VI: 7.8% each) (18). Serotype distribution in other countries as the United States showed the prevalence of five serotypes including Ia, Ib, II, III and V. Lately, predominance of type VIII was noted in Boston, USA (19).…”

Section: Discussionmentioning

confidence: 99%

Prenatal and neonatal Group B Streptococcus screening and serotyping in Lebanon: incidence and implications

Seoud

Nassar

Zalloua

et al. 2010

Acta Obstet Gynecol Scand

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The study aimed at determining the prevalence, risk factors, perinatal transmission, and serotypes of Group B Streptococcus (GBS) among pregnant women and their newborns in Beirut, Lebanon. This was a cross-sectional study of all pregnant women admitted from February to September 2006 to three major hospitals. Overall, 137 of 775 (17.7%) mothers and 50 of 682 newborns (7.3%) tested positive for GBS. Maternal colonization was not associated with maternal age, household income, gravidity, intrapartum fever, preterm labor, or premature rupture of membrane. Transmission rate was 40/120 (30%). Serotype 5 (24.1%) was the most common followed by serotype 1a (15.0%), 3 (14.4%), 2 (11.8%) and 1b (7.5%). Pregnant women in Lebanon appear to have a relatively high prevalence of GBS colonization with no identifiable risk factors for its acquisition. These results could provide basis for the institution of a national policy for universal maternal GBS screening to reduce neonatal morbidity and mortality.

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A Serotype VIII Strain among Colonizing Group B Streptococcal Isolates in Boston, Massachusetts

Cited by 23 publications

References 13 publications

A composite transposon associated with erythromycin and clindamycin resistance in group B Streptococcus

A composite transposon associated with erythromycin and clindamycin resistance in group B Streptococcus

Perinatal Streptococcus agalactiae Epidemiology and Surveillance Targets

Prenatal and neonatal Group B Streptococcus screening and serotyping in Lebanon: incidence and implications

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