A Sesquiterpene, Dehydrocostus Lactone, Inhibits the Expression of Inducible Nitric Oxide Synthase and TNF-α in LPS-Activated Macrophages

Abstract

Nitric oxide (NO) and tumor necrosis factor alpha (TNF-alpha) are the major mediators produced in activated macrophages which contribute to the circulatory failure associated with septic shock. A sesquiterpene lactone compound (dehydrocostus lactone) isolated from the medicinal plant, Saussurea lappa, inhibited the production of NO in lipopolysaccharide (LPS)-activated RAW 264.7 cells by suppressing inducible nitric oxide synthase enzyme expression. This compound also decreased the TNF-alpha level in LPS-activated systems in vitro and in vivo. Thus, dehydrocostus lactone may be a possible candidate for the development of new drugs to treat endotoxemia accompanied by the overproduction of NO and TNF-alpha.

Antihepatotoxic activity of Saussurea lappa extract on D‐galactosamine and lipopolysaccharide‐induced hepatitis in mice

“…The observed protective effects of Sl.Cr might be due to the reported facts of its identifi ed phytochemicals. The compounds from Saussurea lappa root like costunolide and dehydrocostus lactone (Lee et al, 1999), reynosin and santamarine (Cho et al, 1998) and saussureamine (Matsuda et al, 2003) have shown inhibitory effect on expression of TNF-α and NO, respectively, from activated macrophages. Calcium channel blockers have shown hepatoprotection (Farghali et al, 2000) and we have recently identifi ed calcium channel blocking (CCB) constituents in Saussurea lappa (Gilani et al, 2006).…”

Physiological Activity of Coffee Beans and Roasted Black Beans (Rhynchosia nulubilis) Mixture Extracts for Coffee Alternative Beverage Development

“…Macrophages play a central role in mediating a number of different immunopathological phenomena during inflammation by the overproduction of inflammatory mediators, known as Nitric oxide (NO) and prostaglandins (PGs) (Ricciotti et al 2011;Lee et al 1999). Therefore, the inhibition of NO and PGE2 production may have potential therapeutic value when related with inflammation.…”

Chemical compositions extracted fromWikstroemia indicaand their multiple activities

“…NO plays an important role in the inflammatory process, and an inhibitor of NO release may be considered as a therapeutic agent in the inflammatory diseases (Lee et al, 1999) Results of the anti-inflammatory test (Table 5) indicated that the total extraction, petroleum ether fraction and ethyl acetate fraction had different inhibitory activity. Both n-butanol and water fractions showed no inhibitory activity (IC 50 >100 μg/mL).…”