A Set of Standard Differential Blast Isolates (Magnaporthe grisea (Hebert) Barr.) from the Philippines for Rice (Oryza sativa L.) Resistance

Abstract

A total of 119 blast (Magnaporthe grisea (Hebert) Barr.) isolates collected from the Philippines, were characterized for their pathogenicities using 19 rice (Oryza sativa L.) differential varieties (DVs) targeting for 18 resistance genes. These isolates were classified into 31 groups based on the reaction patterns to 9 DVs for targeting 9 resistance genes, Pia, Pib, Pii, Pit, Pita, Pish, Pi3, and Piz-5, at first, and then further divided into 70 pathotypes considering the reaction pattern of the other 9 DVs for the other resistance genes, Pik, Piz, Pi1, and Pi20(t). Twenty isolates that have differentiating ability, stable reactions and good sporulating ability were selected. The reactions of these isolates were confirmed using the monogenic lines as a set of DVs for targeting 24 resistance genes, Pia,

Multiple functional polymorphisms in a single disease resistance gene in rice enhance durable resistance to blast

“…QTL-mediated resistance is often undetectable on account of the race-specific resistance that is induced by pathogen races that are avirulent to cultivars carrying some R genes. As well as differential fungal isolates 44,45 , advancedbackcrossed lines 46,47 are useful for the identification and fine-scale mapping of beneficial QTLs from diverse resources, on account of their efficient incorporation into elite genetic backgrounds without linkage drag 14 . A near-isogenic line for Pi35 (KS-Pi35) is being evaluated in field trials to confirm the absence of undesirable agronomic traits; monitoring of this line will confirm or deny the durability of Pi35-mediated resistance.…”

Resistance Genes and Selection DNA Markers for Blast Disease in Rice (Oryza sativa L.)

“…Telebanco-Yanoria et al 95 surveyed genetic diversity of blast resistance in 922 rice varieties by using the standard differential blast isolates selected by TelebancoYanoria et al 96 . They revealed the relationships among the variations of the pattern of resistance for 20 standard blast isolates, geographical distribution, and the genetic variations characterized by the isozyme types 26 of the rice varieties.…”

Mapping of a QTL for Field Resistance to Blast (Pyricularia oryzae Cavara) in Ingngoppor-tinawon, a Rice (Oryza sativa L.) Landrace from the Philippines

“…Seven days after inoculation, the lesions on the 5th and 4th leaves were counted (Yaegashi 1995) and an inoculation assay using Japanese isolates was conducted at NIAS. Four isolates (CA41, C923-49, M64-1-3-9-1 and V850256) collected from the Philippines (Telebanco-Yanoria et al 2008) were used for an inoculation assay performed in an isolated greenhouse at the Japan International Research Center for Agricultural Sciences (JIRCAS). The method for the Philippine isolates resembled that described for the Japanese isolates.…”

“…Many indica varieties are also useful donors for blast resistance; for example, Pb1 (Panicle blast 1) from the indica rice variety 'Modan' was identified ) and has been widely used for breeding in Japan. To provide a tool for the systematic analysis of blast resistance genes, the International Rice Research Institute (IRRI)-Japan Collaborative Research Project has established 31 rice monogenic lines as a set of differential varieties containing 24 major resistance genes (Kobayashi et al 2007, Telebanco-Yanoria et al 2008, Tsunematsu et al 2000. Moreover, Telebanco-Yanoria et al (2008) selected standard differential blast isolates from the Philippines, and established a differential system for rice blast-resistant study.…”