A severe combined immunodeficiency mutation in the mouse

Bosma, Gayle C.; Custer, R. Philip; Bosma, Melvin J.

doi:10.1038/301527a0

Cited by 2,047 publications

(1,137 citation statements)

References 14 publications

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“…No significant bacterial pathogens were detected. In addition, a C57BL/6 mouse doubly homozygous for the scid (severe combined immune deficiency) mutation 14 and a non-responder allele at the Lps (lipopolysaccharide response) locus 15 has been housed with the germ-line chimaera for over a month and has remained healthy. These results suggest that the phenotype observed in homozygous mutant animals is not due to primary infection by common mouse pathogens, although the possibility of secondary bacterial or viral involvement in the mutant phenotype cannot be eliminated completely.…”

Section: Histopathologymentioning

confidence: 99%

Targeted disruption of the mouse transforming growth factor-β1 gene results in multifocal inflammatory disease

Shull

Ormsby

Kier

et al. 1992

Nature

2,834

1,774

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Section: Histopathologymentioning

confidence: 99%

Targeted disruption of the mouse transforming growth factor-β1 gene results in multifocal inflammatory disease

Shull

Ormsby

Kier

et al. 1992

Nature

2,834

1,774

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“…To assess this, we adoptively transferred, by intraperitoneal inoculation, purified (Fig. 2) BALB/c splenic and peritoneal B-2 cells into congenic CB.17 SCID mice, which are deficient for B and T lymphocytes [30]. Recipient mice were sacrificed 11 days post-inoculation.…”

Section: Peritoneal B-2 Cells Accentuate B-1b-like Characteristics Upmentioning

confidence: 99%

Peritoneal B‐2 cells comprise a distinct B‐2 cell population with B‐1b‐like characteristics

et al. 2006

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B‐1 and B‐2 cells are lymphocyte populations that differ in development, surface marker expression, tissue localization, and function. Though mainly found in the spleen, lymph nodes, and circulation of mice, small numbers of B‐2 cells are found in the peritoneal cavity, a site predominantly populated by B‐1 cells. Here, we characterized peritoneal B‐2 cells, and determined their relationship to B‐1 cells. We found that peritoneal B‐2 cells appear to be intermediate between splenic B‐2 and peritoneal B‐1 cells in terms of surface marker expression of B220, CD80, and CD43, expression of several marker genes, and in vitro viability and IgM secretion. Adoptive transfer of peritoneal B‐2 cells into severe combined immunodeficiency mice resulted in the acquisition of a phenotype reminiscent of B‐1b cells, as shown by up‐regulation of Mac‐1 and CD43, and down‐regulation of CD23. Moreover, adoptively transferred peritoneal B‐2 cells recapitulated B‐1 cell function by producing natural IgM in recipient mice. These data suggest that peritoneal B‐2 cells express some characteristics of B‐1b cells and that this similarity increases with additional time in the peritoneal cavity.

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“…While SCID mice lack mature B-and T-lymphocytes due to a recombinase gene deficiency (Bosma et al, 1983) NOD/SCID mice are additionally characterised by a deficit in NK-cells and functioning macrophages (Shultz et al, 1995). Balb/c-SCID mice were purchased from Bundesgesundheitsamt (Berlin, Germany) NOD/SCID mice from Jackson Laboratories (Bar Harbour, USA).…”

Section: Experimental Animalsmentioning

confidence: 99%

Sensitive PCR method for the detection and real-time quantification of human cells in xenotransplantation systems

et al. 2002

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The sensitive detection of human cells in immunodeficient rodents is a prerequisite for the monitoring of micrometastasis of solid tumours, dissemination of leukaemic cells, or engraftment of haematological cells. We developed a universally applicable polymerase chain reaction method for the detection of a human-specific 850-bp fragment of the a-satellite DNA on human chromosome 17. The method allows the detection of one human cell in 10 6 murine cells and could be established as both, a conventional DNA polymerase chain reaction-assay for routine screening, and a quantitative real-time polymerase chain reaction-assay using TaqMan-methodology. It was applied to the following xenotransplantation systems in SCID and NOD/ SCID mice: (1) In a limiting dilution assay, cells of the MDA-MB 435 breast carcinoma were injected into the mammary fat pad of NOD/SCID mice. It could be shown that 10 cells mouse 71 were sufficient to induce a positive polymerase chain reaction signal in liver and lung tissue 30 days after transplantation as an indicator for micrometastasis. At this time a palpable tumour was not yet detectable in the mammary fat pad region. (2) Cells of a newly established human acute lymphatic leukaemia were administered intraperitoneally to SCID mice. These cells apparently disseminated and were detectable as early as day 50 in the peripheral blood of living mice, while the leukaemia manifestation was delayed by day 140. (3) In a transplantation experiment using mature human lymphocytes we wanted to standardise conditions for a successful survival of these cells in NOD/SCID mice. It was established that at least 5610 7 cells given intravenously were necessary and that the mice had to be conditioned by 2 Gy body irradiation to get positive polymerase chain reaction bands in several organs. (4) Engraftment studies with blood stem cells originating from cytapheresis samples of tumour patients or from cord blood were undertaken in NOD/SCID mice in order to define conditions of successful engraftment and to use this model for further optimisation strategies. The polymerase chain reaction method presented allowed a reliable prediction of positive engraftment and agreed well with the results of immunohistochemical or FACS analysis. All together, the polymerase chain reaction method developed allows a sensitive and reliable detection of low numbers of human cells in immunodeficient hosts. In combination with real-time (TaqMan) technique it allows an exact quantification of human cells. As this method can be performed with accessible material of living animals, follow up studies for the monitoring of therapeutic interventions are possible in which the survival time of mice as evaluation criteria can be omitted.

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A severe combined immunodeficiency mutation in the mouse

Cited by 2,047 publications

References 14 publications

Targeted disruption of the mouse transforming growth factor-β1 gene results in multifocal inflammatory disease

Targeted disruption of the mouse transforming growth factor-β1 gene results in multifocal inflammatory disease

Peritoneal B‐2 cells comprise a distinct B‐2 cell population with B‐1b‐like characteristics

Sensitive PCR method for the detection and real-time quantification of human cells in xenotransplantation systems

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