A sex-specific linkage map of the white shrimp Penaeus (Litopenaeus) vannamei based on AFLP markers

Abstract

We report the construction of sex-specific linkage maps for the white shrimp Penaeus (Litopenaeus) vannamei (Penaeidae; Crustacea). Linkage information was generated using amplified fragment length polymorphism (AFLP) markers in a mapping panel consisting of 42 individuals derived from a commercial cross. We used 103 primer combinations that produced 741 segregating bands. From them, 477 segregated in a 1:1 model, 181 in a 3:1 model, 62 fitted both models and 21 fitted neither model. A total of 394 loci with a 1:1 segregation ratio were mapped to unique positions in the male and female maps using a pseudotestcross strategy. A total of 51 and 47 linkage groups were detected for the male and female maps respectively, in comparison to 44 haploid groups expected from the karyotype. The female map covered 2771 Kosambi units (cM) and was 24% longer than the male map (2116 cM long). The distribution of the markers showed that both maps had low saturation and clustering at short linkage distances. Markers with a distorted segregation were observed as previously reported in other shrimp species. The estimated genomic length indicates that the P. vannamei genome has higher recombination rates than closely related species. We demonstrate the feasibility of implementing molecular techniques

High-Density Linkage Maps and Sex-Linked Markers for the Black Tiger Shrimp (Penaeus monodon)

“…Li et al 2006;Maneeruttanarungroj et al 2006), except for P. vannamei (Perez et al 2004;Zhang et al 2006), more markers could be mapped on the male map than on the female map. The intermarker distance was also slightly lower in the male map and the estimated genome size was larger in the female than in the male map.…”

“…Molecular markers have been used for the analysis of the population structure of wild shrimp resources (for review, see Benzie 2000;Xu et al 2001), strain and species identification (Khamnamtong et al 2005), and parentage analysis (e.g., Jerry et al 2006). First-stage linkage maps have been generated for P. monodon (Wilson et al 2002;Maneeruttanarungroj et al 2006), P. vannamei (Perez et al 2004;Zhang et al 2006), P. chinensis (Z. , and P. japonicus (Moore et al 1999;Li et al 2003). In the case of P. japonicus, a QTL for growth has been identified on the male map (Y. .…”

A first genetic linkage map of bluegill sunfish (Lepomis macrochirus) using AFLP markers

“…This strategy originated from eucalyptus (Eucalyptus grandis) genetic mapping of RAPD markers (Grattapaglia and Sederoff 1994) and has been used in genetic mapping of many aquatic animals Perez et al 2004;Yue et al 2004;Wang et al 2006). In the present study, a double-pseudo testcross strategy was used in the genetic linkage mapping of paternity and maternity of bluegill.…”

“…However, this type of shortage could be avoided in the linkage mapping approach that is based on a pseudo-testcross strategy, since only a 1:1 ratio segregant loci could be recorded in the linkage mapping, and the ''band'' in the electrophoresis pattern of progeny would only correspond to heterozygosity and not to dominant homozygosity. Because of its ability to identify a large number of polymorphic bands without any prior knowledge of DNA sequences in the organism, the AFLP technique is generally accepted as a very powerful and efficient tool in the preliminary genetic linkage mapping in aquatic animals Li et al , 2005Perez et al 2004 LG9 62.6cM (8) LG10 54.5cM (4) LG11 43.4cM (9) LG12 38.6cM (3) LG13 32.6cM (3) LG14 31cM (3) LG15 28.2cM (6) LG2 116cM (10) LG3 106.7cM (8) LG4 89.7cM (9) LG5 81.6cM (3) LG6 76.9cM (6) LG7 75.8cM (5) LG8 72.6cM (6) lm23-529 LG16 28.1cM (4) LG17 25.3cM (7) LG18 23.9cM (4) LG19 23.8cM (3) LG20 23cM (5) LG21 22.4cM (3) LG22 20.3cM (3) LG23 18.7cM (3) LG24 17.5cM (5) LG25 12.2cM (5) LG26 11.7cM (4) LG27 10.7cM (4) LG28 7.2cM (7) LG29 6.8cM (6) LG30 5.9cM (3) LG31 5.8cM (3) LG32 4.6cM (3) LG33 2.3cM (3) Fig. 2 Male linkage groups of AFLP markers of bluegill sunfish (Lepomis macrochirus).…”

BAC end sequencing of Pacific white shrimp Litopenaeus vannamei: a glimpse into the genome of Penaeid shrimp

“…Until now, the genetic maps of L . vannamei consisted primarily of AFLP markers (Perez et al, 2004;Zhang et al, 2007), although several microsatellite markers were also included Garcia and Alcivar-warren, 2007). An SNP-based linkage map was recently generated (Du et al, 2010), but the coverage and density still need further improvements.…”