A Shear horizontal surface acoustic wave biosensor for a rapid and specific detection of d-serine

Pietrantonio, F. Di; Benetti, M.; Cannatà, D.; Verona, É.; Girasole, M.; Fosca, Marco; Dinarelli, Simone; Staiano, Maria; Marzullo, Vincenzo Manuel; Capo, Alessandro; Varriale, Antonio; D’Auria, Sabato

doi:10.1016/j.snb.2015.11.099

Cited by 27 publications

(21 citation statements)

References 21 publications

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“…Such a measurement needs careful experimental design, appropriate analytical techniques and proper execution in order to be confident in differentiating D-serine from other amino acids and from its L-enantiomer in biological samples. Analytical methods have matured, and we now have an array of techniques ranging from biosensors, [25][26][27] high pressure liquid chromatography (HPLC) [28][29][30] and LC/ LC 31 to LC/mass spectrometry 32,33 or capillary electrophoresis-laser-induced fluorescence (CE-LIF) [34][35][36] with well described protocols to detect and measure D-AAs (including D-serine) even at very low levels in complex matrices like biological samples. Despite the availability of multiple approaches to confirm D-serine, analysis of recent publications suggests that this D-AA was not always quantified with the controls that are outlined in Box 1 and Figure 1.…”

Section: Detecting D -Serine Properly With Analytical Methods: That Imentioning

confidence: 99%

Investigating brain d‐serine: Advocacy for good practices

et al. 2019

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The last two decades have witnessed remarkable advance in our understanding the role of D-amino acids in the mammalian nervous system: from the unknown, to known molecules with unknown functions, to potential central players in health and disease. D-Amino acids have emerged as an important class of signaling molecules. In particular, the exploration of the roles of D-serine in brain physiopathology is a vibrant field that is growing at an accelerating pace. However, disentangling the functions of a chiral molecule in a complex chemical matrice as the brain requires specific measurement and detection methods but is also a challenging task as many molecular tools and models investigators are using can lead to confounded observations. Thus, study of D-amino acids demands accurate methodologies and specific controls, and these have often been lacking. Here we outline best practices for D-amino acid research, with a special emphasis on D-serine. We hope these concepts help move the field to greater rigor and reproducibility, allowing the field to advance. K E Y W O R D Sanalytical methods, D-Serine, glia, immunostainings, neurons, NMDA receptors, rescue experiments, serine racemase inhibitors

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Section: Detecting D -Serine Properly With Analytical Methods: That Imentioning

confidence: 99%

Investigating brain d‐serine: Advocacy for good practices

et al. 2019

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“…The sensing properties of a biosensor are related both to the efficiency of the bonding of the biological material to the receptor material, and to the response of the sensor to the variation of the properties of the receptor material which results from this bonding. Gold is widely used in SAW biosensors as the immobilization layer, since it has properties making it suitable for the detection of a large range of analytes [17,18].…”

Section: Introductionmentioning

confidence: 99%

Love Wave Surface Acoustic Wave Sensor with Laser-Deposited Nanoporous Gold Sensitive Layer

Viespe

Dinca

Popescu-Pelin

et al. 2019

Sensors

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Laser-deposited gold immobilization layers with different porosities were incorporated into Love Wave Surface Acoustic Wave sensors (LW-SAWs). Acetylcholinesterase (AChE) enzyme was immobilized onto three gold interfaces with different morphologies, and the sensor response to chloroform was measured. The response of the sensors to various chloroform concentrations indicates that their sensing properties (sensitivity, limit of detection) are considerably improved when the gold layers are porous, in comparison to a conventional dense gold layer. The results obtained can be used to improve properties of SAW-based biosensors by controlling the nanostructure of the gold immobilization layer, in combination with other enzymes and proteins, since the design of the present sensor is the same as that for a Love Wave biosensor.

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“…The insertion phase angle versus frequency is another very important characteristic of the device, which is also critically important for analysis and detection. Most prior work only reported results based on phase angle shift to different mass loading [39,40]. After the complex number of the propagated wave is calculated from the voltage and current of the output electrodes, the phase angle of z can be calculated from the equation listed in Table 3.…”

Section: Resultsmentioning

confidence: 99%

Finite Element Analysis for Surface Acoustic Wave Device Characteristic Properties and Sensitivity

Wang

Green

Guldiken

et al. 2019

Sensors

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The most vital step in the development of novel and existing surface acoustic wave (SAW)-based sensors and transducers is their design and optimization. Demand for SAW devices has been steadily increasing due to their low cost, portability, and versatility in electronics, telecommunications, and biosensor applications. However, a full characterization of surface acoustic wave biosensors in a three-dimensional (3D) finite element model has not yet been developed. In this study, a novel approach is developed for analyzing shear horizontal Love wave resonator devices. The developed modeling methodology was verified using fabricated devices. A thorough analysis of the 3D model and the experimental device was performed in this study including scattering parameters (S-parameters), reflection coefficient parameters, transmission parameters, and phase velocity. The simulated results will be used as a design guideline for future device design and optimization, which has thus far resulted in close matching between prediction and experimental results. This manuscript is the first to demonstrate a 3D finite element model to correlate the sensitivity of the SAW device with the magnitude of the phase shift, the real and imaginary part of the response, insertion loss, and the frequency shift. The results show that the imaginary part of the response shift has a higher sensitivity compared to other parameters.

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A Shear horizontal surface acoustic wave biosensor for a rapid and specific detection of d-serine

Cited by 27 publications

References 21 publications

Investigating brain d‐serine: Advocacy for good practices

Investigating brain d‐serine: Advocacy for good practices

Love Wave Surface Acoustic Wave Sensor with Laser-Deposited Nanoporous Gold Sensitive Layer

Finite Element Analysis for Surface Acoustic Wave Device Characteristic Properties and Sensitivity

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