A short peptide from frog skin accelerates diabetic wound healing

Abstract

Delayed wound healing will result in the development of chronic wounds in some diseases, such as diabetes. Amphibian skins possess excellent wound‐healing ability and represent a resource for prospective wound‐healing promoting compounds. A potential wound‐healing promoting peptide (CW49; amino acid sequence APFRMGICTTN) was identified from the frog skin of Odorrana grahami. It promotes wound healing in a murine model with a full‐thickness dermal wound in both normal and diabetic animals. In addition to its strong angiogenic ability with respect to the upregulation of some angiogenic proteins, CW49 also showed a significant anti‐inflammatory effect in diabetic wounds, which was very important for healing chronic wounds. CW49 had little effect on re‐epithelialization, resulting in no significant effect on wound closure rate compared to a vehicle control. Altogether, this indicated that CW49 might accelerate diabetic wound healing by promoting angiogenesis and preventing any excessive inflammatory response. Considering its favorable traits as a small peptide that significantly promotes angiogenesis, CW49 might be an excellent candidate or template for the development of a drug for use in the treatment of diabetic wounds.

Anterior gradient 2 is induced in cutaneous wound and promotes wound healing through its adhesion domain

“…by regulating the formation of focal adhesions. These AGR2 functions in wound healing involve FAK and JNK signaling pathways and require AGR2 adhesion domain (amino acids [21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40]. Our finding indicates that external AGR2 may represent an alternative strategy for the development of therapeutics for wounds with large exposure area or difficult-to-heal chronic wounds.…”

A frog cathelicidin peptide effectively promotes cutaneous wound healing in mice

“…One is tylotoin, which was isolated from the salamander Tylototriton verrucosus (Caudata: Salamandridae) [ 17 ]; the other is cathelicidin-OA1, which was identified from the Chinese odorous frog Odorrana andersonii (Anura: Ranidae) [ 18 ]. Additionally, two non-cathelicidin peptides (CW49 and AH90) from frog skin secretions and one designed peptide (tiger17) based on frog antimicrobial peptides, also showed wound-healing ability [ 19 – 21 ].…”

Bv8-Like Toxin from the Frog Venom of Amolops jingdongensis Promotes Wound Healing via the Interleukin-1 Signaling Pathway

“…Over recent years, a number of amphibian peptides such as AH90, βγ-CAT, Ot-WHP, CW49, and Bm-TFF2 has been discovered and they have been reported to play important roles in angiogenesis and wound healing [20][21][22][23][24]. AH90 was identified from skin secretions of frog Odorrana grahami.…”