A short peptide potentially promotes the healing of skin wound

Abstract

Skin wound, a common form of skin damage in daily life, remains a serious challenge in clinical treatment. Bioactive peptides with high efficiency have been considered as potential therapeutic candidates for wound healing. In this report, a novel short linear peptide, with mature peptide sequence of ‘GLLSGINAEWPC’ and no obvious similarity with other known bioactive peptides, was identified by genomic method from the skin of odorous frog, Odorrana andersonii. Our results suggested that OA-GL12 (OA: abbreviation of species (O. andersonii), GL: two initial amino acids, 12: peptide length) obviously accelerated the scratch-healing of keratinocytes and human fibroblasts in a time- and concentration-dependent manner. Meanwhile, OA-GL12 showed significant effect in promoting the wound healing on the full-thickness skin wound model. Inflammatory assay results demonstrated that OA-GL12 induced the secretion of tumor necrosis factor (TNF) and transforming growth factor β1 (TGF-β1) on murine macrophage cell line (RAW264.7), which might explain the powerful accelerating capacity of wound healing. Moreover, results also indicated that epidermal growth factor receptor (EGFR) was involved in the mechanisms underlying the scratch-healing promoting activity of OA-GL12. In addition, OA-GL12 showed obvious free radical scavenging activity. Results supported that OA-GL12 did not exert risk in acute toxicity, hemolytic activity, and direct antibacterial activity. The remarkable effect of OA-GL12 on promoting wound healing verified in this research made it potential to be a novel template for the development of wound healing-promoting agents.

Evaluation of Small Molecular Polypeptides from the Mantle of Pinctada Martensii on Promoting Skin Wound Healing in Mice

“…Nutritional supplements are generally absorbed by the wound on oral administration [15]. Therefore, the method of applying SMPs may limit the absorption of macromolecular nutrients, such as proteins, but smear administration would hydrate the stratum corneum, increase permeability, and enhance drug absorption [16]. At the same time, small peptides, trace elements, and other bioactive substances directly act on the skin wounds, thus shortening the reaction time and accelerating the healing process through smearing [16].…”

“…Therefore, the method of applying SMPs may limit the absorption of macromolecular nutrients, such as proteins, but smear administration would hydrate the stratum corneum, increase permeability, and enhance drug absorption [16]. At the same time, small peptides, trace elements, and other bioactive substances directly act on the skin wounds, thus shortening the reaction time and accelerating the healing process through smearing [16]. In this study, hydrophobic amino acids accounted for 56.51% of the total amino acids, and hydrophilic amino acids accounted for 31.3% of the total amino acids.…”

Peptides from Animal Origin: A Systematic Review on Biological Sources and Effects on Skin Wounds

“…Due to the complete skin removal, all phases of tissue repair are more pronounced in excisional than in incisional wounds [ 50 ]. Thus, excisional injuries are widely used in second intention healing models [ 22 , 26 , 31 , 40 ]. In these cases, the intense inflammatory process and the marked tissue remodeling favor the analysis of the effectiveness of healing products [ 26 ].…”

The Therapeutic Effect and In Vivo Assessment of Palmitoyl- GDPH on the Wound Healing Process

“…Skin is the largest organ that covers the body and acts as a physical barrier between the inner and outer environment. As an external barrier, it is always exposed to the adverse effects following physical injury such as infection, shock, and even death [ 1 ]. Therefore, it is vital to the body to mount an immediate response to the injury in order to restore the skin function through an effective wound healing process.…”