A short review on the research progress in alfalfa leaf protein separation technology

Zhang, Wenxiang; Grimi, Nabil; Jaffrin, Michel Y.; Ding, Luhui; Tang, Bing

doi:10.1002/jctb.5364

Cited by 33 publications

(20 citation statements)

References 21 publications

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“…Alfalfa ( Medicago sativa L.) has one of the highest feeding values of leguminous forages (Zhang et al ., 2017), and is an excellent feed ingredient preserved as hay, haylage or silage for lactating dairy cows (Schmidt et al ., 2009). In comparison with grasses, it has a higher content of crude protein (CP), greater concentration of organic acids and minerals; however, it is difficult to ensile due to its high buffering capacity for acidic conditions, in combination with the low water-soluble carbohydrates (WSC) concentrations (McDonald et al ., 1991; Ogunade et al ., 2016).…”

Section: Introductionmentioning

confidence: 99%

The role of homofermentative and heterofermentative lactic acid bacteria for alfalfa silage: a meta-analysis

et al. 2020

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Lactic acid bacteria (LAB) are usually employed as alfalfa silage inoculants to obtain high-quality feed for animal husbandry. However, the effects of these inoculants are still unclear and need to be studied extensively. Therefore, the objective of this meta-analysis was to quantitatively summarize published research studies that assess the effects of homofermentative (HoLAB) and heterofermentative lactic acid bacteria (HeLAB) on fermentation parameters, nutritive value, microbiological composition and aerobic stability of alfalfa silage. PubMed, ScienceDirect and Scopus have been screened for articles published from 1980 to 2018. The criteria for inclusion were: randomized and controlled trials using alfalfa silage and published in peer-reviewed journals. It was found that inoculation with LAB decreased silage pH, neutral detergent fibre, acid detergent fibre and ammoniacal nitrogen, while it increased dry matter and crude protein compared to control in the pooled raw mean difference random-effect model. Additionally, LAB inoculation decreased acetate, propionate, ethanol and butyrate concentrations, whereas it increased lactate. In addition, inoculants reduced the counts of yeasts and moulds. Lastly, LAB inoculation improved aerobic stability. To the best of our knowledge, this is the first meta-analysis that aims at comparing the application of HoLAB and HeLAB for alfalfa silage. In the pool estimate, positive effects attributable to the application of microbial silage inoculants were found in most of the evaluated parameters; supporting the importance of applying both types of inoculants to improve forage preservation for the livestock industry.

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Section: Introductionmentioning

confidence: 99%

The role of homofermentative and heterofermentative lactic acid bacteria for alfalfa silage: a meta-analysis

et al. 2020

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“…Despite those distinguished features, HPTFF is vulnerable to bigger MWCO membrane. This trend was confirmed by Zhang et al that testing UF rotating disk membrane (UF-RDM) for separation and concentration of leaf protein from alfalfa juice [23,145,146]. Higher MWCO and temperature was attributed to aggravating irreversible fouling and separation performance during VRF = 6 and VRF = 12, but also to leveraging better filtration behavior and productivity.…”

Section: High-performance Tangential Flow Filtration (Hptff)mentioning

confidence: 61%

Recent Advancements of UF-Based Separation for Selective Enrichment of Proteins and Bioactive Peptides—A Review

et al. 2021

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Proteins are one of the primary building blocks that have significant functional properties to be applied in food and pharmaceutical industries. Proteins could be beneficial in their concentrated products or isolates, of which membrane-based filtration methods such as ultrafiltration (UF) encompass application in broad spectra of protein sources. More importantly, selective enrichment by UF is of immense interest due to the presence of antinutrients that may dominate their perspicuous bioactivities. UF process is primarily obstructed by concentration polarization and fouling; in turn, a trade-off between productivity and selectivity emerges, especially when pure isolates are an ultimate goal. Several factors such as operating conditions and membrane equipment could leverage those pervasive contributions; therefore, UF protocols should be optimized for each unique protein mixture and mode of configuration. For instance, employing charged UF membranes or combining UF membranes with electrodialysis enables efficient separation of proteins with a similar molecular weight, which is hard to achieve by the conventional UF membrane. Meanwhile, some proposed strategies, such as utilizing ultrasonic waves, tuning operating conditions, and modifying membrane surfaces, can effectively mitigate fouling issues. A plethora of advancements in UF, from their membrane material modification to the arrangement of new configurations, contribute to the quest to actualize promising potentials of protein separation by UF, and they are reviewed in this paper.

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“…Heat precipitation yielded the lowest precipitation yield, on average 33.5%, whereas isoelectric precipitation at pH 4-4.5 precipitated 34.0%. Based on the average isolectric point of all proteins in green juice, the optimum for isolectric precipitation is typically pH 4-4.5 (Santamaría-Fernández et al, 2017;Zhang et al, 2017). The results obtained here revealed that precipitation at a lower pH of 2.5, i.e., the pH used for lignosulfonate precipitation, gave a small improvement of the precipitation by 4%.…”

Section: Loading Experiments From Ryegrass-clovermentioning

confidence: 71%

“…A key step in the green biorefinery is the protein separation. Typically, the green biomass is fractionated into two different fractions by pressing in e.g., a screw press (Zhang et al, 2017;Corona et al, 2018a). One fraction is the pulp, which contains fibers along with insoluble and fiber bound proteins.…”

Section: Introductionmentioning

confidence: 99%

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Enhancing Protein Recovery in Green Biorefineries by Lignosulfonate-Assisted Precipitation

Cour

Schjøerring

Jørgensen

2019

Front. Sustain. Food Syst.

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Biorefining of green biomass to produce proteins for feed and food provides an important challenge in relation to development of a future sustainable and climateneutral agriculture. In order to make it a viable value chain, all parts of the process need to be optimized. This includes careful selection of the source of biomass, the procedure used for protein extraction as well as the separation and recovery of proteins from the press juice. We here focus on recovery of proteins from juice made from screw press fractionation of four green biomasses, viz. ryegrass, red clover, ryegrasswhite clover mixture and spinach. Separating out protein from the juice is a key step and several different methods have routinely been applied. We demonstrate that lignosulfonates aid in the precipitation of proteins in the juice. The optimal loading of lignosulfonate was 0.6-0.7 g per g crude protein in the juice. For ryegrass and red clover juice, this loading resulted in 10-25% increase in precipitated protein compared to the classical precipitation by heat treatment or acidification. For spinach, up to 60% increase in protein recovery was obtained by lignosulfonate addition. We conclude that lignosulfonate-assisted precipitation holds potential for improving the protein yield in biorefining of green biomass.

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A short review on the research progress in alfalfa leaf protein separation technology

Cited by 33 publications

References 21 publications

The role of homofermentative and heterofermentative lactic acid bacteria for alfalfa silage: a meta-analysis

The role of homofermentative and heterofermentative lactic acid bacteria for alfalfa silage: a meta-analysis

Recent Advancements of UF-Based Separation for Selective Enrichment of Proteins and Bioactive Peptides—A Review

Enhancing Protein Recovery in Green Biorefineries by Lignosulfonate-Assisted Precipitation

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