A Short Review on Various Screening Methods to Isolate Potential Lipase Producers: Lipases-the Present and Future Enzymes of Biotech Industry

Abstract

The increased demand for microbial originated Industrial enzymes especially lipases which have received least priority till last one and half decades and gained lots of importance in recent days owing to their applications in wide variety of fields such as food, dairy, pharmaceutical, detergent, textile, oleo-chemical, perfume and cosmetic industries etc., has lead to the identification of novel enzymes from new sources with unique properties. The present review mainly focuses on various currently available screening methods for identification of lipase producers.

Diversity, structure, and synteny of the cutinase gene ofColletotrichumspecies

“…Bioassays confirmed the ability of C. truncatum and C. brevisporum to produce and secrete cutinase enzyme in lipid substrate media. Growth characteristics for the identification of secreted lipase activity using Tween‐20, Rhodamine, and tributyrin agar plates were congruent with the results obtained from other studies (Lanka & Latha, ; Ramnath, Sithole, & Govinden, ; Wadia & Jain, ). As both Tween‐20 and Tributyrin can test positive with esterases, the positive fluorescence with Rhodamine B was used as the determining factor for the presence of lipase activity (Lanka & Latha, ; Tomulescu et al, ).…”

“…Growth characteristics for the identification of secreted lipase activity using Tween‐20, Rhodamine, and tributyrin agar plates were congruent with the results obtained from other studies (Lanka & Latha, ; Ramnath, Sithole, & Govinden, ; Wadia & Jain, ). As both Tween‐20 and Tributyrin can test positive with esterases, the positive fluorescence with Rhodamine B was used as the determining factor for the presence of lipase activity (Lanka & Latha, ; Tomulescu et al, ). Bornscheuer () explained that lipases can be differentiated from carboxyl esterases based on their substrate spectra: p ‐nitrophenyl palmitate ( p NPP) is hydrolyzed by lipases; p ‐nitrophenyl butyrate ( p NPB) is hydrolyzed by esterases.…”

“…Bioassays confirmed the ability of C. truncatum and C. brevisporum to produce and secrete cutinase enzyme in lipid substrate media. Growth characteristics for the identification of secreted lipase activity using Tween-20, Rhodamine, and tributyrin agar plates were congruent with the results obtained from other studies (Lanka & Latha, 2015;Ramnath, Sithole, & Govinden, 2017;Wadia & Jain, 2017). As both Tween-20 and…”

“…Tributyrin can test positive with esterases, the positive fluorescence with Rhodamine B was used as the determining factor for the presence of lipase activity (Lanka & Latha, 2015;Tomulescu et al, 2015). Bornscheuer (2002) explained that lipases can be differentiated from carboxyl esterases based on their substrate spectra: p-nitrophenyl palmitate (pNPP) is hydrolyzed by lipases; p-nitrophenyl butyrate (pNPB) is hydrolyzed by esterases.…”

Avanços em caracterização bioquímica de lipases microbianas: uma revisão

“…Dada a grande especificidade, versatilidade e propriedades seletivas apresentadas pelas lipases estas enzimas estão se tornando cada vez mais significativas em várias aplicações industriais. Algumas das aplicações das enzimas lipolíticas podem ser vistas na área farmacêutica, na síntese de produtos químicos finos e de oleoquímicos, nas indústrias alimentícia, de couro, cosméticos, detergentes, no desenvolvimento de flavors, de fragrâncias, no pré-tratamento de águas residuais ricas em lipídios, na bioremediação de solos contaminados Research, Society and Development, v. 9, n. 4, e104942897, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i4.2897 4 por óleos/gorduras e na síntese de biodiesel (Priji et al, 2016;Melani, Tambourgi e Silveira, 2019;Lanka & Latha, 2015;Ramos-Sánchez et al, 2015;Javed et al, 2018;Sharma, Sharma e Saxena, 2016).…”