A sick plot–based protocol for dry root rot disease assessment in field‐grown chickpea plants

Irulappan, Vadivelmurugan; Mali, Komal Vitthalrao; Patil, B. S.; Manjunatha, H.; Saifulla, Muhammad; Senthil‐Kumar, Muthappa

doi:10.1002/aps3.11445

Cited by 11 publications

(4 citation statements)

References 31 publications

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“…The pathogen load in the sick plot was enriched over successive crop seasons by adding more pathogen culture in plot. The percentage of wilt incidence was calculated based on the initial plant count and the total number of wilted plants (Irulappan et al., 2021).…”

Section: Methodsmentioning

confidence: 99%

Development and evaluation of Fusarium wilt‐resistant and high‐yielding chickpea advanced breeding line, KCD 11

Laxuman,

Naik,

Desai

et al. 2024

The Plant Genome

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Fusarium wilt (FW) is the most severe soil‐borne disease of chickpea that causes yield losses up to 100%. To improve FW resistance in JG 11, a high‐yielding variety that became susceptible to FW, we used WR 315 as the donor parent and followed the pedigree breeding method. Based on disease resistance and yield performance, four lines were evaluated in station trials during 2017–2018 and 2018–2019 at Kalaburagi, India. Further, two lines, namely, Kalaburagi chickpea desi 5 (KCD 5) and KCD 11, which possesses the resistance allele for a specific single‐nucleotide polymorphism marker linked with FW resistance, were evaluated across six different locations (Bidar, Kalaburagi, Raichur, Siruguppa, Bhimarayanagudi and Hagari) over a span of 3 years (2020–2021, 2021–2022 and 2022–2023). KCD 11 exhibited notable performance, showcasing yield advantages of 8.67%, 11.26% and 23.88% over JG 11, and the regional checks Super Annigeri 1 (SA 1) and Annigeri 1, respectively, with enhanced FW resistance in wilt sick plot. Further, KCD 11 outperformed JG 11, SA 1 and Annigeri 1 in multi‐location trials conducted across three seasons in the North Eastern Transition Zone, North Eastern Dry Zone, and North Dry Zones of Karnataka. KCD 11 was also tested in trials conducted by All India Coordinated Research Project on chickpea and was also nominated for state varietal trials for its release as a FW‐resistant and high‐yielding variety. The selected line is anticipated to cater the needs of chickpea growers with the dual advantage of yield increment and disease resistance.

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Section: Methodsmentioning

confidence: 99%

Development and evaluation of Fusarium wilt‐resistant and high‐yielding chickpea advanced breeding line, KCD 11

Laxuman,

Naik,

Desai

et al. 2024

The Plant Genome

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“…The previously described method (Irulappan et al, 2021) was followed to estimate the fungal DNA present inside the root. M. phaseolina ‐specific primers were used to quantify the fungal load.…”

Section: Methodsmentioning

confidence: 99%

MicroRNA397 regulates tolerance to drought and fungal infection by regulating lignin deposition in chickpea root

Sharma

Yadav

Gupta

et al. 2023

Plant Cell & Environment

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Plants deposit lignin in the secondary cell wall as a common response to drought and pathogen attacks. Cell wall localised multicopper oxidase family enzymes LACCASES (LACs) catalyse the formation of monolignol radicals and facilitate lignin formation. We show an upregulation of the expression of several LAC genes and a downregulation of microRNA397 (CamiR397) in response to natural drought in chickpea roots. CamiR397 was found to target LAC4 and LAC17L out of twenty annotated LACs in chickpea. CamiR397 and its target genes are expressed in the root. Overexpression of CamiR397 reduced expression of LAC4 and LAC17L and lignin deposition in chickpea root xylem causing reduction in xylem wall thickness. Downregulation of CamiR397 activity by expressing a short tandem target mimic (STTM397) construct increased root lignin deposition in chickpea. CamiR397‐overexpressing and STTM397 chickpea lines showed sensitivity and tolerance, respectively, towards natural drought. Infection with a fungal pathogen Macrophomina phaseolina, responsible for dry root rot (DRR) disease in chickpea, induced local lignin deposition and LAC gene expression. CamiR397‐overexpressing and STTM397 chickpea lines showed more sensitivity and tolerance, respectively, to DRR. Our results demonstrated the regulatory role of CamiR397 in root lignification during drought and DRR in an agriculturally important crop chickpea.

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“…The genotypes were evaluated under sick plot at the Regional Research Center for Soybean, under Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Amravati, Maharashtra State (20° 55’ 53.82” N. 77° 45’ 32.57” E ). The disease incidence percentage was determined using the formula outlined [30]. The disease index was studied using a scale of 0-5 score.…”

Section: Methodsmentioning

confidence: 99%

Unravelling Silicon’s Transcriptomic Armor in Soybean againstMacrophomina phaseolinacausing Charcoal Rot Disease

Jadhav,

Magar,

Sharma

et al. 2023

Preprint

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TheGlycine maxL. has been affected by more than 100 diseases, includingMacrophomina phaseolinaproducing charcoal rot disease, which reduces production by 70%. In this investigation, RNA-Seq analysis is used for the first time to explore role of silicon in preventing soybean charcoal rot. The study explores the molecular mechanism underlying soybeans’ resilience to charcoal rot when treated with potassium silicon. It was meticulously investigated howMacrophomina phaseolinaentered the roots. The SEM, which showed a strong link between potassium silicate accumulation and disease resistance. Further investigation indicates that a potassium silicate concentration of 1.7mM lowers disease incidence. Using Illumina HiSeq NGS data, we present a transcriptome analysis revealing genes associated with charcoal rot resistance, highlighting 3,106 genes with distinct expression patterns. The strong enrichment of pathways including “Biosynthesis of ansamycins” and “Flavone and flavonol biosynthesis,” which contribute to resistance against charcoal rot, is highlighted by KEGG enrichment analysis. The ERF transcription factor and NB leucine-rich repeats stands out among the differentially expressed genes as being particularly connected to resistance. The crucial functions that many other important transcription factors, including as MYB, NAC, and proteins from the FAR1 family, play in enhancing soybeans’ resistance to charcoal rot are also noted. This newly discovered information could help in developing tactics to strengthen soybean’s resistance toMacrophomina phaseolina.

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A sick plot–based protocol for dry root rot disease assessment in field‐grown chickpea plants

Cited by 11 publications

References 31 publications

Development and evaluation of Fusarium wilt‐resistant and high‐yielding chickpea advanced breeding line, KCD 11

Development and evaluation of Fusarium wilt‐resistant and high‐yielding chickpea advanced breeding line, KCD 11

MicroRNA397 regulates tolerance to drought and fungal infection by regulating lignin deposition in chickpea root

Unravelling Silicon’s Transcriptomic Armor in Soybean againstMacrophomina phaseolinacausing Charcoal Rot Disease

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