A side-by-side comparison of the performance and time-and-motion data of VITEK MS

Bardelli, Marta; Padovani, Michela; Fiorentini, Simona; Caruso, Arnaldo; Yamamura, Deborah; Gaskin, Mark; Jissam, Ali; González-López, Juan José; Larrosa, Nieves; Pumarola, Tomás; Lassus, Anna; Louis, Barbara; Capron, Nicolas

doi:10.1007/s10096-022-04472-x

Cited by 7 publications

(7 citation statements)

References 16 publications

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“…Despite these limitations, we documented the ability of the VMS-P system to correctly identify microorganisms commonly isolated in a clinical microbiology laboratory. These results are consistent with those of Bardelli, et al [12], who recently showed that VITEK MS PRIME performs similarly to VMS and reduces the time required for pathogen identification. In the future, it would be interesting to conduct a workflow study that defines the time necessary to obtain an identification, beginning at the spotting step until a result is obtained on the laboratory information system.…”

Section: Discussionsupporting

confidence: 92%

“…We compared the performance of VMS-P, a new MALDI-TOF MS system, and the MBT system currently used in our laboratory. While the new VMS-P system had been recently compared with the first VITEK MS [12], to our knowledge, this is the first comparative evaluation between this new platform and the MBT system. In routine daily practice, both the VMS-P and MBT systems performed similarly, with 97.8% agreement in terms of microorganism identification at the species level, when used acwww.annlabmed.org https://doi.org/10.3343/alm.2023.43.6.574 cording to the manufacturer's recommendations.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of the New VITEK MS PRIME System with the Matrix-Assisted Laser Desorption Ionization Biotyper Microflex LT for the Identification of Microorganisms

Grohs,

Remaud,

Lath

et al. 2023

Ann Lab Med

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Background: Matrix-assisted laser desorption ionization (MALDI) is the cornerstone of bacterial identification. The performance of a new MALDI time-of-flight mass spectrometry VI-TEK MS PRIME (VMS-P) system was compared with that of the MALDI Biotyper Microflex LT (MBT) system, which is routinely used in our laboratory.Methods: Sixteen bacterial and yeast reference strains cultured in 20 different media were analyzed over 10 consecutive rounds using both systems. Bacterial and yeast isolates from the routine workflow were processed using both systems. Microcolonies were identified after a 4-hour agar subculture from positive blood culture bottles, without extraction.Results: To determine the repeatability based on the reference strains, 1,190 spots were processed using each system. Correct identification was achieved for 94.0% (MBT) and 98.4% (VMS-P; P < 0.01) of spots. Among these, 83.0% (MBT) and 100.0% (VMS-P) were identified with a high degree of confidence. For 1,214 spots from routine isolates, species identification was achieved for 90.0% (MBT) and 91.4% (VMS-P; P = 0.26) of spots. For 69.8% (MBT) and 87.4% (VMS-P) of the spots, identification was achieved with a high degree-of-confidence score. When identification was performed using both systems, the agreement between them was 97.9%. The identification of microcolonies from positive blood culture bottles was achieved for 55.5% (MBT) and 70.2% (VMS-P; P = 0.01) of spots. Conclusions:The MBT and VMS-P systems perform similarly in routine daily practice. The new VMS-P system shows high repeatability, better confidence scores for identification, and promising ability to identify microcolonies.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Comparison of the New VITEK MS PRIME System with the Matrix-Assisted Laser Desorption Ionization Biotyper Microflex LT for the Identification of Microorganisms

Grohs,

Remaud,

Lath

et al. 2023

Ann Lab Med

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“…Fundamental to the effective treatment of bacterial infections is choosing an antibiotic to which the pathogen is susceptible. The level of susceptibility is readily estimated by culture methods, both through automation via BioMerieux's VITEK and similar devices (3)(4)(5)(6), as well as by non-automated methods such as disk diffusion and Epsilon-diffusion tests (7,8). By these methods, bacteria are classified as susceptible, intermediate, or resistant according to the international consensus guidelines from the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST).…”

Section: Main Text Introductionmentioning

confidence: 99%

Theoretical Considerations and Empirical Predictions of the Pharmaco- and Population Dynamics of Heteroresistance

Levin,

Berryhill,

Gil-Gil

et al. 2023

Preprint

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Background Antibiotics are considered one of the most important contributions to clinical medicine in the last 100 years. As a consequence of the use and overuse of these drugs, there have been increasing frequencies of infections with resistant pathogens. One form of resistance, heteroresistance, is particularly problematic; pathogens appear sensitive to a drug by commonly used susceptibility tests. However, upon exposure to the antibiotic, resistance rapidly ascends, and treatment fails. Methods We generate two distinct mathematical and computer-simulations models of heteroresistance to quantitatively explore the factors contributing to the emergence and ascent of resistance during treatment and the waning of resistance following cessation of treatment. We consider the factors that determine the response to antibiotic-mediated selection. In one model, heteroresistance is progressive, with each resistant state sequentially generating a higher resistance level. In the other model, heteroresistance is non-progressive, with a susceptible population directly generating populations with different resistance levels. Findings The conditions where resistance will ascend in the progressive model are narrower than those of the non-progressive model. The rate of reversion from the resistant to the sensitive states are critically dependent on the transition rates and the fitness cost of resistance. Our results demonstrate that the standard test used to identify heteroresistance is insufficient. The predictions of our models are consistent with empirical results. Interpretations Our results demand a reevaluation of the definition and criteria employed to identify heteroresistance. We recommend the definition of heteroresistance should include a consideration of the rate of return to susceptibility. Funding Funds for this study were provided by the U.S. National Institute of General Medical Sciences, the U.S. National Institute of Allergy and Infectious Diseases, and the Carlos III Institute of Health of Spain.

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“…The VITEK MS PRIME (bioMérieux, Marcy l'Etoile, France) is a new version of the VITEK MALDI-TOF MS instrument developed by bioMérieux. This upgraded instrument features a continuous load-and-go sample loading system, prioritization for critical patient samples on urgent slides, and new internal components, all of which are designed to enhance sample processing speed and reduce handling time [8]. The VITEK MS PRIME system utilizes VITEK MS IVD Database 3.2., but it also allows for additional analysis using SARAMIS ® Knowledge Base v4.…”

Section: Introductionmentioning

confidence: 99%

Clinical Evaluation of VITEK MS PRIME with PICKME Pen for Bacteria and Yeasts, and RUO Database for Filamentous Fungi

Lee,

Koo,

et al. 2024

Microorganisms

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The VITEK MS PRIME (bioMérieux, Marcy-l’Étoile, France), a newly developed matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) system, alongside the VITEK PICKME pen (PICKME), offers easy sample preparation for bacteria and yeasts. The VITEK MS PRIME also offers two software platforms for filamentous fungi: the IVD database and the RUO database. Our study evaluated its identification agreement on 320 clinical isolates of bacteria and yeasts, comparing PICKME and traditional wooden toothpick sampling techniques against MicroIDSys Elite (ASTA) results. Additionally, we assessed the IVD (v3.2) and SARAMIS (v4.16) RUO databases on 289 filamentous fungi against molecular sequencing. The concordance rates for species-level identification of bacteria and yeasts were about 89.4% (286/320) between the PICKME and wooden toothpick, and about 83.4–85.3% between the VITEK MS PRIME and ASTA MicroIDSys Elite. Retesting with PICKME improved concordance to 91.9%. For filamentous fungi, species-level identification reached 71.3% with the IVD database and 85.8% with RUO, which significantly enhanced basidiomycetes’ identification from 35.3% to 100%. Some strains in the IVD database, like Aspergillus versicolor, Exophiala xenobiotica, and Nannizzia gypsea, failed to be identified. The VITEK MS PRIME with PICKME offers reliable and efficient microorganism identification. For filamentous fungi, combined use of the RUO database can be beneficial, especially for basidiomycetes.

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A side-by-side comparison of the performance and time-and-motion data of VITEK MS

Cited by 7 publications

References 16 publications

Comparison of the New VITEK MS PRIME System with the Matrix-Assisted Laser Desorption Ionization Biotyper Microflex LT for the Identification of Microorganisms

Comparison of the New VITEK MS PRIME System with the Matrix-Assisted Laser Desorption Ionization Biotyper Microflex LT for the Identification of Microorganisms

Theoretical Considerations and Empirical Predictions of the Pharmaco- and Population Dynamics of Heteroresistance

Clinical Evaluation of VITEK MS PRIME with PICKME Pen for Bacteria and Yeasts, and RUO Database for Filamentous Fungi

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