A signal-flexible gene diagnostic strategy coupling loop-mediated isothermal amplification with hybridization chain reaction

Dong, Qing; Liu, Quanyi; Guo, Lulu; Li, Dan; Shang, Xudong; Li, Bingling; Du, Yan

doi:10.1016/j.aca.2019.06.048

Cited by 29 publications

(17 citation statements)

References 51 publications

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“…Bst 2.0 DNA polymerase and 10 Â Isothermal Buffer (10 Â Iso) were obtained from New England Biolabs (Ipswich, MA, USA). Thermostable invertase (TmINV) derived from the hyperthermophilic bacteria, Thermotoga maritima, was home-made according to our previous publication without modification [20,21]. All the oligonucleotides (listed in Table S3) and ompA and malB synthetic DNA templates were synthesized by Sangon Biotech (Shanghai, China).…”

Section: Chemicals and Materialsmentioning

confidence: 99%

One-tube smart genetic testing via coupling isothermal amplification and three-way nucleic acid circuit to glucometers

Guo

Dong

et al. 2020

Analytica Chimica Acta

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Section: Chemicals and Materialsmentioning

confidence: 99%

One-tube smart genetic testing via coupling isothermal amplification and three-way nucleic acid circuit to glucometers

Guo

Dong

et al. 2020

Analytica Chimica Acta

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“…Unlike typical thermal cycling amplification, isothermal techniques are carried out at a constant temperature, which reduces the power needed and makes them more compatible for integration into miniaturised systems [ 4 ]. These techniques have provided biosensors with the relevant sensitivity and specificity for the detection of different analytes such as microalgae [ 6 , 7 ] viruses [ 8 , 9 ], fungi [ 10 , 11 ], protozoa [ 12 , 13 ] and bacteria, being the latter the focus of this review. Therefore, the objective of this work is to provide a comprehensive overview of the biosensors based on isothermal DNA amplification methods for bacterial detection with application in the fields of food safety and environmental monitoring, ultimately affecting human, animal and plant health.…”

Section: Introductionmentioning

confidence: 99%

Biosensors Based on Isothermal DNA Amplification for Bacterial Detection in Food Safety and Environmental Monitoring

Leonardo

Toldrà

Campàs

2021

Sensors

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The easy and rapid spread of bacterial contamination and the risk it poses to human health makes evident the need for analytical methods alternative to conventional time-consuming laboratory-based techniques for bacterial detection. To tackle this demand, biosensors based on isothermal DNA amplification methods have emerged, which avoid the need for thermal cycling, thus facilitating their integration into small and low-cost devices for in situ monitoring. This review focuses on the breakthroughs made on biosensors based on isothermal amplification methods for the detection of bacteria in the field of food safety and environmental monitoring. Optical and electrochemical biosensors based on loop mediated isothermal amplification (LAMP), rolling circle amplification (RCA), recombinase polymerase amplification (RPA), helicase dependent amplification (HDA), strand displacement amplification (SDA), and isothermal strand displacement polymerisation (ISDPR) are described, and an overview of their current advantages and limitations is provided. Although further efforts are required to harness the potential of these emerging analytical techniques, the coalescence of the different isothermal amplification techniques with the wide variety of biosensing detection strategies provides multiple possibilities for the efficient detection of bacteria far beyond the laboratory bench.

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“…[9,10] A short DNA strand can be extended using DNA polymerases and techniques such as rolling circle amplification (RCA). [11][12][13][14][15][16][17][18] RCA has been widely used in many areas of research. [19][20][21][22][23] Some signal amplification strategies based on RCA have been applied to achieve sensitive detection of markers at low levels.…”

Section: Introductionmentioning

confidence: 99%

Protein detection based on rolling circle amplification sensors

Shi

Cui

Sulemana³

et al. 2021

Luminescence

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Rolling circle amplification (RCA) is an isothermal process under the action of DNA polymerases. Large-scale DNA templates have been generated using RCA for target detection. Some signal amplification strategies including optical sensors and electrochemical sensors based on RCA have been applied to achieve sensitive detection.Sensors based on RCA have attracted increasing interest. Advances in RCA-based sensors for protein detection are reviewed in this paper. The advantages and detection mechanisms of sensors based on RCA are revealed and discussed. Finally, possible challenges and future perspectives are also outlined.

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A signal-flexible gene diagnostic strategy coupling loop-mediated isothermal amplification with hybridization chain reaction

Cited by 29 publications

References 51 publications

One-tube smart genetic testing via coupling isothermal amplification and three-way nucleic acid circuit to glucometers

One-tube smart genetic testing via coupling isothermal amplification and three-way nucleic acid circuit to glucometers

Biosensors Based on Isothermal DNA Amplification for Bacterial Detection in Food Safety and Environmental Monitoring

Protein detection based on rolling circle amplification sensors

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