A signal-on magnetic electrochemical immunosensor for ultra-sensitive detection of saxitoxin using palladium-doped graphitic carbon nitride-based non-competitive strategy

Jin, Xin; Chen, Jianlang; Zeng, Xiaoxue; Xu, Li; Wu, Yongning; Fu, FengFu

doi:10.1016/j.bios.2018.12.036

Cited by 55 publications

(16 citation statements)

References 42 publications

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“…This LSPR aptasensor showed a broad detection linear range from 5 to 10,000 μg/L with a high sensitivity determined by an LOD value of 2.46 μg/L. These sensing performances are sufficient for a STX sensor, because EFSA suggests that STX should remain in seafood below 75 μg/kg to ensure safety [8]. Moreover, the proposed LSPR aptasensor was applied to real sample analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Saxitoxin (STX), which is the most representative PSP, binds to the voltage-gated Na + channel of neurons and blocks neuronal transmission between nerves and muscle, and this can cause fetal illness, e.g., cardiovascular shock, respiratory arrest, and even death [5,6,7]. For these dangers, a guideline of 75 ug/kg STX in seafood has been suggested by the European Food Safety Authority (FESA) [8]. Several methods are available for the detection of STX.…”

Section: Introductionmentioning

confidence: 99%

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Label-Free Direct Detection of Saxitoxin Based on a Localized Surface Plasmon Resonance Aptasensor

Park

Lee

et al. 2019

Toxins

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Seafood is an emerging health food, and interest in improving the quality of seafood is increasing. Saxitoxin (STX) is a neurotoxin produced by marine dinoflagellates that is accumulated in seafood. It can block the neuronal transmission between nerves and muscle cell membranes, resulting in the disturbance of neuromuscular transmission and subsequent voluntary muscle paralysis. Here, we developed a new aptamer for the detection of STX using graphene oxide–systematic evolution of ligands by exponential enrichment (GO-SELEX). Furthermore, we confirmed sensitivity and selectivity of the developed aptamer specific to STX using a localized surface plasmon resonance (LSPR) sensor. The sensing chip was fabricated by fixing the new STX aptamer immobilized on the gold nanorod (GNR) substrate. The STX LSPR aptasensor showed a broad, linear detection range from 5 to 10,000 μg/L, with a limit of detection (LOD) of 2.46 μg/L (3σ). Moreover, it was suitable for the detection of STX (10, 100, and 2000 μg/L) in spiked mussel samples and showed a good recovery rate (96.13–116.05%). The results demonstrated that the new STX aptamer-modified GNR chip was sufficiently sensitive and selective to detect STX and can be applied to real samples as well. This LSPR aptasensor is a simple, label-free, cost-effective sensing system with a wide detectable range.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Label-Free Direct Detection of Saxitoxin Based on a Localized Surface Plasmon Resonance Aptasensor

Park

Lee

et al. 2019

Toxins

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“…Palladium-doped graphitic carbon nitride nanosheets (g-C 3 N 4 -PdNPs) were also found to have outstanding intrinsic peroxidase-like activity [99]. This nanocomposite was used as label for the detection of saxitoxin (STX), a relevant paralytic shellfish toxin at pg/mL levels [100] (Figure 7B).…”

Section: Nanoparticles As Electrocatalytic Labelsmentioning

confidence: 99%

“…Adapted from [98] with permission; ( B ) (left) Scheme of a non-competitive magnetic electrochemical immunosensor for STX quantification based on the H 2 O 2 mediated oxidation of 3,3’,5,5’-Tetramethylbenzidine (TMB) and the current-time curves for detecting different concentration of STX, with the calibration curve as inset. Adapted from [100] with permission.…”

Section: Figurementioning

confidence: 99%

Nanoparticles as Emerging Labels in Electrochemical Immunosensors

Iglesias-Mayor

Amor-Gutiérrez

Costa-Garcı́a

et al. 2019

Sensors

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This review shows recent trends in the use of nanoparticles as labels for electrochemical immunosensing applications. Some general considerations on the principles of both the direct detection based on redox properties and indirect detection through electrocatalytic properties, before focusing on the applications for mainly proteins detection, are given. Emerging use as blocking tags in nanochannels-based immunosensing systems is also covered in this review. Finally, aspects related to the analytical performance of the developed devices together with prospects for future improvements and applications are discussed.

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“…The ingestion of contaminated vectors causes neurotoxic illness that can result in paralysis and, at its acute expression, death. With this target in mind, Jin and co-workers [27] developed a magnetic electrochemical immunosensor for the detection of STX in seawater and seafood. The immunosensor used anti-STX antibody-functionalized MBs and palladium-doped graphitic carbon nitride nanoparticles (peroxidase mimetic) to generate the electrochemical signal.…”

Section: Magnetic Beads As Supportsmentioning

confidence: 99%

Magnetic Beads in Marine Toxin Detection: A Review

2019

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Due to the expanding occurrence of marine toxins, and their potential impact on human health, there is an increased need for tools for their rapid and efficient detection. We give an overview of the use of magnetic beads (MBs) for the detection of marine toxins in shellfish and fish samples, with an emphasis on their incorporation into electrochemical biosensors. The use of MBs as supports for the immobilization of toxins or antibodies, as signal amplifiers as well as for target pre-concentration, is reviewed. In addition, the exploitation of MBs in Systematic Evolution of Ligands by Exponential enrichment (SELEX) for the selection of aptamers is presented. These MB-based strategies have led to the development of sensitive, simple, reliable and robust analytical systems for the detection of toxins in natural samples, with applicability in seafood safety and human health protection.

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A signal-on magnetic electrochemical immunosensor for ultra-sensitive detection of saxitoxin using palladium-doped graphitic carbon nitride-based non-competitive strategy

Cited by 55 publications

References 42 publications

Label-Free Direct Detection of Saxitoxin Based on a Localized Surface Plasmon Resonance Aptasensor

Label-Free Direct Detection of Saxitoxin Based on a Localized Surface Plasmon Resonance Aptasensor

Nanoparticles as Emerging Labels in Electrochemical Immunosensors

Magnetic Beads in Marine Toxin Detection: A Review

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