A silk fibroin/chitosan/nanohydroxyapatite biomimetic bone scaffold combined with autologous concentrated growth factor promotes the proliferation and osteogenic differentiation of BMSCs and repair of critical bone defects

Zhou, Yi; Liu, Xiaoyan; She, Hongjiang; Wang, Rui; Bai, Fan; Xiang, Bingyan

doi:10.1016/j.reth.2022.08.006

Cited by 23 publications

(15 citation statements)

References 52 publications

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“…The porosity of normal human bone is 50–90% . In order to meet the supply of nutrients required for cell growth as well as the exchange of other substances, the porosity of the bone repair scaffold should not be less than 70% . In this study, we selected a dual-drug-loaded hydrogel with a porosity size of 90.12 ± 3.27%, which meets the high porosity requirement for bone tissue regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…Then, these tubes were placed in a constant temperature shaker (MTHYHC2000-T, Quatek, China) (140 rpm, 37 °C). The hydrogels were removed from the test tubes on days 1, 3,5,7,9,11,13,15,17,20,23,26, and 29, dried, and weighed (W t ), while the solutions in the tubes were replenished. Each group of hydrogels was tested five times.…”

Section: Hydrogel Degradation Behavior Testmentioning

confidence: 99%

“…Bone tissue engineering is regarded as an ideal way for repairing bone defects. It provides scaffolds for the site of the defect, while these scaffolds provide a good three-dimensional space for cell survival, matrix formation, and bone remodeling. , A bone repair scaffold with good performance needs to have (1) good biocompatibility, (2) biodegradability, (3) certain mechanical properties, (4) osteoconductivity, (5) certain porosity and swelling, and (6) induction of cell migration, proliferation, and surface properties for differentiation. − Hydrogels are promising materials for bone repair because of their high water content, high porosity, and nutrient permeability; furthermore, they can simulate the extracellular matrix (ECM) to promote cell growth . Poly(β-amino ester) (PBAE) is a synthetic biodegradable polymer, which is obtained from various primary or secondary diamines with diacrylate monomers by Michael addition reaction .…”

Section: Introductionmentioning

confidence: 99%

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Poly(β-amino ester) Dual-Drug-Loaded Hydrogels with Antibacterial and Osteogenic Properties for Bone Repair

Cheng

Wang

et al. 2023

ACS Biomater. Sci. Eng.

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In this study, we developed a poly(β-amino ester) (PBAE) hydrogel for the double release of vancomycin (VAN) and total flavonoids of Rhizoma Drynariae (TFRD). VAN was covalently bonded to PBAE polymer chains and was released to enhance the antimicrobial effect first. TFRD chitosan (CS) microspheres were physically dispersed in the scaffold, TFRD was released from the microspheres, and osteogenesis was induced subsequently. The scaffold had good porosity (90.12 ± 3.27%), and the cumulative release rate of the two drugs in PBS (pH 7.4) solution exceeded 80%. In vitro antimicrobial assays demonstrated the antibacterial properties of the scaffold against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Besides these, cell viability assays indicated that the scaffold had good biocompatibility. Moreover, alkaline phosphatase and matrix mineralization were expressed more than in the control group. Overall, cell experiments confirmed that the scaffolds have enhanced osteogenic differentiation capabilities. In conclusion, the dual-drug-loaded scaffold with antibacterial and bone regeneration effects is promising in the field of bone repair.

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Section: Discussionmentioning

confidence: 99%

Section: Hydrogel Degradation Behavior Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Poly(β-amino ester) Dual-Drug-Loaded Hydrogels with Antibacterial and Osteogenic Properties for Bone Repair

Cheng

Wang

et al. 2023

ACS Biomater. Sci. Eng.

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“…The composite scaffold presented the compression strength of 0.82 ± 0.05 MPa, which was lower than the mechanical strength value of trabecular bone (2–20 MPa). Nevertheless, scaffolds with similar or lower compression strength reported in literature were able to provide adequate support as templates during the first phase of bone generation [ 39 , 40 ]. The composite scaffolds were biodegraded and replaced by the new bone.…”

Section: Discussionmentioning

confidence: 99%

β-tricalcium phosphate/gelatin composite scaffolds incorporated with gentamycin-loaded chitosan microspheres for infected bone defect treatment

Liu¹,

Zhao²,

Chen³

et al. 2022

PLoS ONE

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The repair of infected bone defects remains a clinical challenge. Staphylococcus aureus is a common pathogenic micro-organism associated with such infections. Gentamycin (GM) is a broad spectrum antibiotic that can kill S. aureus in a dose-dependent manner. However, the systemic administration of antibiotics may lead to drug resistance and gut dysbiosis. In this work, we constructed β-tricalcium phosphate/gelatin composite scaffolds incorporated with gentamycin-loaded chitosan microspheres (CMs(GM)-β-TCP/gelatin composite scaffolds), which helped optimize the local GM release in the infected defect areas and enhance bone regeneration. The cumulative release curves showed that both microspheres and composite scaffolds reached a sustained slow-release phase after the initial rapid release, and the latter further stabilized the initial drug release rate. The release curve of CMs(GM)-β-TCP/gelatin composite scaffolds reached a plateau after 24 h, and the cumulative release reached 41.86% during this period. Moreover, the combination of β-TCP and gelatin mimicked bone composition and were able to provide the requisite mechanical strength (0.82 ± 0.05 MPa) during the first phase of bone generation. The inner structure of the scaffold was arranged in the shape of interconnected pores, and presented a porosity level of 16%. The apertures were uniform in size, which was beneficial for cell proliferation and material transportation. Macroscopic observation and histological analysis showed that CMs(GM)-β-TCP/gelatin composite scaffolds fused with bone tissues, and new tissues were formed in defect areas without any infection. This new composite scaffold may be a promising repair material for treating infected bone defects.

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“…Os scaffolds a base de quitosana usados na regeneração de tecidos duros, uma condição crítica é sua capacidade de suporte de carga, por ser um polímero flexível, a quitosana não atende às propriedades mecânicas dos ossos naturais. Desta forma, a HAp possibilita a superação desse obstáculo, conferindo a rigidez mecânica adequada ao sistema, formando assim o scaffolds de quitosana/HAp (10)(11)(12)(13)(14)(15)(16)(17).…”

Section: Introductionunclassified

Scaffolds Magnéticos De Quitosana E/Ou Hidroxiapatita Para Regeneração De Tecido Ósseo: Uma Revisão Sistemática

Guedes¹,

Cunha²,

Araújo³

et al. 2023

RECIMA21

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A incorporação e a exploração de partículas com propriedades magnéticas como um potencializador dos processos de regeneração óssea por meio de scaffolds tridimensionais tem sido uma estratégia na engenharia de tecido ósseo, o que possibilita obtenção de estruturas com capacidade responsiva à estimulos externos, inteligentes e bioativos. Nesse sentido, este artigo propõe uma revisão sistemática baseada em artigos de pesquisa primária com foco em scaffolds magnéticos de quitosana e/ou hidroxiapatita na regeneração do tecido ósseo. Com este fim foi realizada uma busca nas plataformas Science Direct, Pubmed e Web of Science no período entre 2020 a 2022 e a revisão foi conduzida por meio do software Start. Critérios de inclusão foram utilizados para a seleção dos periódicos mais relevantes e observou-se que dentre as estratégias de scaffolds magnéticos de quitosana e/ou hidroxiapatita reportadas, as mais significativas envolveram indução da osteogenese endocondral, aumento nas vias de formação de vasos sanguíneos no tecido ósseo lesado, sistema de liberação de fármaco, entrega de fatores de crescimento e combate de células tumorais. Os resultados demonstraram esses sistemas apresentam potencial promissor de aplicação em regeneração óssea.

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A silk fibroin/chitosan/nanohydroxyapatite biomimetic bone scaffold combined with autologous concentrated growth factor promotes the proliferation and osteogenic differentiation of BMSCs and repair of critical bone defects

Cited by 23 publications

References 52 publications

Poly(β-amino ester) Dual-Drug-Loaded Hydrogels with Antibacterial and Osteogenic Properties for Bone Repair

Poly(β-amino ester) Dual-Drug-Loaded Hydrogels with Antibacterial and Osteogenic Properties for Bone Repair

β-tricalcium phosphate/gelatin composite scaffolds incorporated with gentamycin-loaded chitosan microspheres for infected bone defect treatment

Scaffolds Magnéticos De Quitosana E/Ou Hidroxiapatita Para Regeneração De Tecido Ósseo: Uma Revisão Sistemática

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