A silk fibroin hydrogel with reversible sol–gel transition

Yin, Zhuping; Wu, Feng; Xing, Tieling; Yadavalli, Vamsi K.; Kundu, Subhas C.; Lu, Shenzhou

doi:10.1039/c7ra02682j

Cited by 54 publications

(69 citation statements)

References 57 publications

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“…The results indicated that the hydrophilicity of the blended films improved with increasing the regenerated silk fibroin proportions. The observation demonstrated that the regenerated silk fibroin has significant influence on the wettability of the blended surfaces, which can be explained by the entanglement of RSF molecules and the exposure of their hydrophilic groups, which may be arranged on the surface of RSF chains [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Phase Behaviour and Miscibility Studies of Collagen/Silk Fibroin Macromolecular System in Dilute Solutions and Solid State

et al. 2017

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Miscibility is an important issue in biopolymer blends for analysis of the behavior of polymer pairs through the detection of phase separation and improvement of the mechanical and physical properties of the blend. This study presents the formulation of a stable and one-phase mixture of collagen and regenerated silk fibroin (RSF), with the highest miscibility ratio between these two macromolecules, through inducing electrostatic interactions, using salt ions. For this aim, a ternary phase diagram was experimentally built for the mixtures, based on observations of phase behavior of blend solutions with various ratios. The miscibility behavior of the blend solutions in the miscible zones of the phase diagram was confirmed quantitatively by viscosimetric measurements. Assessing the effects of biopolymer mixing ratio and salt ions, before and after dialysis of blend solutions, revealed the importance of ion-specific interactions in the formation of coacervate-based materials containing collagen and RSF blends that can be used in pharmaceutical, drug delivery, and biomedical applications. Moreover, the conformational change of silk fibroin from random coil to beta sheet, in solution and in the final solid films, was detected by circular dichroism (CD) and Fourier transform infrared spectroscopy (FTIR), respectively. Scanning electron microscopy (SEM) exhibited alterations of surface morphology for the biocomposite films with different ratios. Surface contact angle measurement illustrated different hydrophobic properties for the blended film surfaces. Differential scanning calorimetry (DSC) showed that the formation of the beta sheet structure of silk fibroin enhances the thermal stability of the final blend films. Therefore, the novel method presented in this study resulted in the formation of biocomposite films whose physico-chemical properties can be tuned by silk fibroin conformational changes by applying different component mixing ratios.

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Section: Resultsmentioning

confidence: 99%

Phase Behaviour and Miscibility Studies of Collagen/Silk Fibroin Macromolecular System in Dilute Solutions and Solid State

et al. 2017

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“…Furthermore, the 15 mM DMPG–SF hydrogels reached the failure point at the lowest strain value when compared with the other formulations (Figure h). Yin et al () proposed that not only the beta sheet structure is responsible for the viscoelasticity of SF hydrogels, but also the weak and reversible hydrogen bonds. Certainly, hydrogen bonds can be formed between the polar head of DMPG (Garidel, Blume, & Hübner, ) and SF.…”

Section: Discussionmentioning

confidence: 99%

Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration

Laomeephol

Guedes

Ferreira

et al. 2019

J Tissue Eng Regen Med

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Silk fibroin (SF) hydrogels can be obtained via self-assembly, but this process takes several days or weeks, being unfeasible to produce cell carrier hydrogels. In this work, a phospholipid, namely, 1,2-dimyristoyl-sn-glycero-3-phospho-(1′-rac-glycerol) sodium salt (DMPG), was used to induce and accelerate the gelation process of SF solutions. Due to the amphipathic nature and negative charge of DMPG, electrostatic and hydrophobic interactions between the phospholipids and SF chains will occur, inducing the structural transition of SF chains to the beta sheet and consequently a rapid gel formation is observed (less than 50 min). Moreover, the gelation time can be controlled by varying the lipid concentration. To assess the potential of the hydrogels as cell carriers, several mammalian cell lines, including L929, NIH/3T3, SaOS-2, and CaSki, were encapsulated into the hydrogel. The silk-based hydrogels supported the normal growth of fibroblasts, corroborating their cytocompatibility.Interestingly, an inhibition in the growth of cancer-derived cell lines was observed.Therefore, DMPG-induced SF hydrogels can be successfully used as a 3D platform for in situ cell encapsulation, opening promising opportunities in biomedical applications, such as in cell therapies and tissue regeneration.Bombyx mori "Nangnoi Srisaket 1" Thai silk cocoons were obtained from Queen Sirikit Sericulture Center, Srisaket province, Thailand.DMPG and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) were purchased from Avanti Lipids Polar, USA. Cell culture medium and all other reagents were purchased from Thermo Fisher Scientific.All reagents were of analytical grade. | SF solution preparation and its zeta potential determinationSF solution was prepared using the method adapted from Kim, Park, Joo Kim, Wada, and Kaplan (2005). In brief, silk cocoons were boiled in 0.02 M Na 2 CO 3 for 20 min, followed by their thoroughly washing with distilled water. The extracted silk fibre was then dissolved in 9.3 M LiBr at 60°C for 4 hr. The silk solution was dialysed against distilled water using a dialysis tube (MWCO 12-16 kDa; Sekisui, Japan) for 48 hr. The final concentration was approximately 6%, which was determined from dried solid weight.Analysis of zeta potential of the SF solution was conducted using laser doppler electrophoresis in a Zetasizer Nano ZS equipment (Malvern Instruments) at 25°C.

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“…It was demonstrated that SS plays a crucial role in the antibacterial process of wound treatment. The sol-gel transition of aqueous solution of SF is a natural process which without an exterior stimulus is quite long, usually a week to a month, which may limit its practical use [323]. Therefore, there are some factors that can stimulate and enhance the gelation kinetics of SF.…”

Section: Silkmentioning

confidence: 99%

New Developments in Medical Applications of Hybrid Hydrogels Containing Natural Polymers

Vasile¹,

Pamfil²,

Stoleru³

et al. 2020

Molecules

211

131

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New trends in biomedical applications of the hybrid polymeric hydrogels, obtained by combining natural polymers with synthetic ones, have been reviewed. Homopolysaccharides, heteropolysaccharides, as well as polypeptides, proteins and nucleic acids, are presented from the point of view of their ability to form hydrogels with synthetic polymers, the preparation procedures for polymeric organic hybrid hydrogels, general physico-chemical properties and main biomedical applications (i.e., tissue engineering, wound dressing, drug delivery, etc.).

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A silk fibroin hydrogel with reversible sol–gel transition

Abstract: Herein, we prepare a novel silk fibroin hydrogel with a reversible thixotropic gel–sol transition triggered by a facile cycled shearing and resting procedure.

Cited by 54 publications

References 57 publications

Phase Behaviour and Miscibility Studies of Collagen/Silk Fibroin Macromolecular System in Dilute Solutions and Solid State

Phase Behaviour and Miscibility Studies of Collagen/Silk Fibroin Macromolecular System in Dilute Solutions and Solid State

Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration

New Developments in Medical Applications of Hybrid Hydrogels Containing Natural Polymers

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