A simple and easy method for determination of meloxicam in rat muscle and plasma

Abstract

The aim of this paper is to provide a simple and easy method for determination of meloxicam in rat muscle and plasma, based on dissolution of tissue samples with Solvable TM and measurement with a column-switching high performance liquid chromatographic system. After tissue and plasma samples were extracted with Solvable TM and methanol, samples were injected into a precolumn (Hypersil ODS) in 0.05 M phosphate buffer (pH 3), and after clean-up and enrichment the analytes were transferred to an analytical column (YMC Pack Pro C18) by backflushing with 0.05 M phosphate buffer (pH 6)-methanol (60:40, v/v). The eluate was monitored with a UV-detector set at 360 nm. Coefficients of determination (r 2) for muscle and plasma were both more than 0.999. The limits of quantification (LOQ) in muscle and plasma were 50 ng/g and 20 ng/mL, respectively. The accuracy and precision were achieved in the range of 50-2500 ng/g in muscle and 20-2500 ng/mL in plasma. Our method could be successfully applied to evaluate the pharmacokinetics of topically administrated meloxicam.

Considerations of inductively coupled plasma mass spectrometry techniques for characterizing the dissolution of metal-based nanomaterials in biological tissues

“…Handling animal tissues with suitable pretreatment procedures is the most critical step for further evaluation of the chemical fate of MNMs. Recently, treatment of animal tissues with alkaline Solvable™ solution (a mixture of dodecyldimethylamine oxide, secondary alcohol ethoxylate, and sodium hydroxide in water; 83,84 used as a commercial tissue solubilizer to facilitate sample measurements for liquid scintillation counting) has been applied successfully to the extraction of AgNPs, QDs, and their released ions from intact biological tissues. 68,69,71 Doudrick et al compared eight chemical treatment methods used commonly to extract metal ions from complex matrices-Solvable™, ammonium hydroxide, nitric acid, sulfuric acid, hydrochloric acid (HCl), hydrouoric acid, hydrogen peroxide, and proteinase K-for liberating the CNTs from rat lung tissues.…”