A simple and efficient miracidium hatching technique for preparing a          single-genome DNA sample of &lt;i&gt;Schistosoma japonicum&lt;/i&gt;

Wanlop, Atcharaphan; Dang-Trinh, Minh-Anh; Kirinoki, Masashi; Suguta, Saki; Shinozaki, Kaho; Kawazu, Shin-ichiro

doi:10.1292/jvms.21-0536

Cited by 4 publications

(1 citation statement)

References 13 publications

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“…mansoni cercariae were obtained from 3 Swiss albino mice which were already infected and on life cycle maintenance at the Institute. Livers from 3 mice were emulsified and filtered in nested sieves to obtain a filtrate containing ova which was then illuminated using a lamp to hatch as described by Wanlop et al [27]. The eggs were hatched into miracidia and were used to infect 30 B. pfeifferi snails of 4mm diameter using a routine optimized technique.…”

Section: Maintenance Of S Mansoni Parasitementioning

confidence: 99%

Immunomodulating Potential of Ekebergia capensis in Murine Schistosoma mansoni Juvenile and Adult Infection

Musili,

Lusweti,

Kinyatta

et al. 2024

JABB

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Schistosomiasis is a neglected tropical disease occurring in sub-Saharan Africa and affecting almost 250 million people. The drug of choice for treatment for Schistosomiasis has been Praziquantel which has been used for many years and there is a need to develop new drugs. The immunomodulatory potential of Ekebergia capensis extract on both juvenile and adult Schistosoma mansoni infection in vivo was evaluated in this study. Swiss albino mice were infected individually with 90 S. mansoni cercariae and randomized into groups of five each for i) plant extract treated groups ii) positive control groups treated with conventional drugs PZQ or artemether iii) infected but untreated (negative control) groups. The mice were treated orally with aqueous extracts of E. capensis at doses of 200 and 400 mg/kg at 2 weeks (juvenile worms) and 7 weeks (adult worms) post-infection. Immune immune-enhancing potential of the medicinal plant was determined by analyzing the levels of cytokines in serum samples that were collected before and after treatment. A BD-Cytometric Bead Array (CBA) mouse Th1/Th2/Th17 kit was used to quantitate the levels of cytokines using a flow cytometer (FACS Calibur) and analysis of the data was done using FCAP software. Results indicated that the medicinal plant extract had an immunomodulatory effect. There was a significant increase (P<0.05) in Th1 cytokines (IL-2, IFN- γ and TNF-α), a decrease in Th2 cytokines (IL-4, IL-6 and IL-10) and an increase in Th17 (IL-17). These findings confirm the potential use of medicinal plants in the management of schistosomiasis.

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Section: Maintenance Of S Mansoni Parasitementioning

confidence: 99%

Immunomodulating Potential of Ekebergia capensis in Murine Schistosoma mansoni Juvenile and Adult Infection

Musili,

Lusweti,

Kinyatta

et al. 2024

JABB

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A compartmental model for Schistosoma japonicum transmission dynamics in the Philippines

Kuo,

Paras,

Tagami

et al. 2024

Acta Tropica

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A Recent Advance in the Diagnosis, Treatment, and Vaccine Development for Human Schistosomiasis

Chatterji,

Khanna,

Alghamdi

et al. 2024

TropicalMed

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Schistosomiasis, which affects a large number of people worldwide, is among the most overlooked parasitic diseases. The disease is mainly prevalent in sub-Saharan Africa, southeast Asian countries, and South America due to the lack of adequate sanitation. The disease is mainly associated with poor hygiene, sanitation, and contaminated water, so it is also known as a disease of poverty. Three Schistosoma species (S. mansoni, S. japonicum, and S. haematobium) cause significant human infections. Co-infections with Schistosoma and other parasites are widely common. All these parasites may cause intestinal or urogenital schistosomiasis, where the disease may be categorized into the acute, sensitized, and chronic phases. The disease is more prevalent among school children, which may cause anemia and reduce development. Chronic infections frequently cause significant liver, intestinal, and bladder damage. Women exposed to contaminated water while performing normal duties like washing clothes might acquire urogenital schistosomiasis (UGS), which can cause tissue damage and raise the risk of blood-borne disease transmission, including human immunodeficiency virus (HIV) transmission. Praziquantel (PZQ) is the World Health Organization (WHO)-prescribed treatment for individuals who are known to be infected, but it does not prevent further re-infections with larval worms. Vaccine development and new molecular-based diagnosis techniques have promised to be a reliable approach to the diagnosis and prevention of schistosomiasis. The current review emphasizes the recent advancement in the diagnosis of schistosomiasis by molecular techniques and the treatment of schistosomiasis by combined and alternative regimes of drugs. Moreover, this review has also focused on the recent outbreak of schistosomiasis, the development of vaccines, and their clinical trials.

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A simple and efficient miracidium hatching technique for preparing a single-genome DNA sample of <i>Schistosoma japonicum</i>

Cited by 4 publications

References 13 publications

Immunomodulating Potential of Ekebergia capensis in Murine Schistosoma mansoni Juvenile and Adult Infection

Immunomodulating Potential of Ekebergia capensis in Murine Schistosoma mansoni Juvenile and Adult Infection

A compartmental model for Schistosoma japonicum transmission dynamics in the Philippines

A Recent Advance in the Diagnosis, Treatment, and Vaccine Development for Human Schistosomiasis

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